Blood fungal infection detection kit and application thereof

A detection kit and fungal infection technology, which is applied in the field of clinical blood microscopic detection, can solve the problems of unfavorable diagnosis and treatment plan for patients with blood infection, long time-consuming, easy to be contaminated, etc.

Inactive Publication Date: 2021-06-08
济宁市第一人民医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Conventional white light microscopy has defects such as missed judgments and misjudgments, and is highly dependent on the experience of the inspectors, while the conventional in vitro culture method takes a long time, has high requirements for the culture environment and culture methods, and is easy to be contaminated. It is not conducive to the diagnosis of patients with blood infection and the formulation of treatment plans. It is urgent to develop rapid and highly sensitive blood fungal diagnostic technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The detection of a blood fungal infection test kit is a solvent, including the following concentration composition: fluorescent calcium 0.005 mmol / L, Ivans Blue 0.0025mol / L, potassium hydroxide 1.78mmol / L, glycerin Fluorescence Diversity 10.87 mmol / L, bisphospholipidyl glycerol 0.033 mmol / L and ascorbic acid 0.057 mmol / L;

[0019] The kit provided by the present invention is used to take 5 μl of healthy blood samples to the slide using a pipette, and 2 μL of the above detection dye is added thereto, mixing with a pipette. After covering the cover slide, 3 sample smears are formed, and the three samples are observed under a fluorescence microscope of 380 to 410 nm excited light, and the three sample smears have not been observed in the circular spore or long. The strip has a germisylid, which proves that the health blood sample is negative.

Embodiment 2

[0021] The detection of a blood fungal infection test kit is a solvent, including the following concentration composition: fluorescent calcium 0.01 mmol / L, Ivans Blue 0.005mmol / L, potassium hydroxide 10.7mmol / L, glycerin Anti-fluorescence decreased 16.3 mmol / L, bisphosphatidyl glycerol 0.033 mmol / L and ascorbic acid 0.057 mmol / L;

[0022] The kit provided by the present invention is used to take 5 μl of healthy blood samples to the slide using a pipette, and 2 μL of the above detection dye is added thereto, mixing with a pipette. After covering the cover slide, 3 sample smears are formed, and the three samples are observed under a fluorescence microscope of 380 to 410 nm excited light, and the three sample smears have not been observed in the circular spore or long. The strip has a germisylid, which proves that the health blood sample is negative.

Embodiment 3

[0024] The detection of a blood fungal infection test kit is a solvent, including the following concentration composition: fluorescent calcium 0.005 mmol / L, Ivans Blue 0.0025mol / L, potassium hydroxide 1.78mmol / L, glycerin Fluorescence Diversity 10.87 mmol / L, bisphospholipidyl glycerol 0.033 mmol / L and ascorbic acid 0.057 mmol / L;

[0025] The medical fungi laboratory fungus is accommodated, and 100 spores were added to the blood every 500 μl of blood, and then the kit provided by the present invention, the method provided by the present invention is used. 5 μL of simulated infection of blood samples to the slide, and additionally use the pipette to add 2 μl of the above detection dyeing liquid, mixed, cover the cover slide, and produce 3 sample smears, which are placed in 380 ~ 410 nm excited light fluorescence microscope observed, 3 sample smears were observed to brightened white spores, which proved that the simulated infection of blood sample positives.

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PUM

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Abstract

The present invention relates to the technical field of clinical blood microscopic detection, and provides a blood fungal infection detection kit, which comprises a detection dye solution, and the detection dye solution uses water as a solvent, and comprises the following components by concentration: 0.005-0.01 mmol / L of fluorescent calcium, 0.0025-0.005 mmol / L of Evans blue, 1.78-10.7 mmol / L of potassium hydroxide, 10.87-16.3 mmol / L of a glycerol anti-fluorescence decay agent, 0.033 mmol / L of diphosphatidyl glycerol, and 0.057 mmol / L of ascorbic acid. The blood fungal infection detection kit and the detection dye liquor used in the blood fungal infection detection kit have the advantages of being low in cost, simple and rapid to prepare and easy to store, fungal infection in blood can be rapidly detected with high sensitivity, the positive detection rate is higher, the limitation of the prior art is solved, and the kit has important clinical value for diagnosis of blood fungal infection patients.

Description

Technical field [0001] The present invention relates to the field of clinical blood microscopic testing, and more particularly to a blood fungal infection testing kit and its application thereof. Background technique [0002] As immunized chases continue to expand, the large number of broad-spectrum antibiotics, the development of invasive technology, the incidence of blood fungal infections continues to increase, seriously threatening the safety of patients. Among them, the systemic fungal infection of high-risk populations, the disease progression is rapid, delayed diagnosis will delay the treatment opportunity, greatly increase the patient mortality. Therefore, early rapid detection of blood fungal infection is extremely important for clinicians' correct diagnosis and timely development of treatment strategies. [0003] At present, conventional in vitro culture methods are routine detection methods of blood fungi infection. However, the traditional method is time-long, the sen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N1/30
CPCG01N21/6402G01N21/6447G01N1/2813G01N1/30G01N2001/302
Inventor 史冬梅陈标边绍东冯亚辉
Owner 济宁市第一人民医院
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