High-sensitivity weak fluorescence signal detection system and method, storage medium and application

A fluorescent signal, high-sensitivity technology, applied in the field of biomedical imaging, can solve the problems of technical cost, imaging quality and imaging speed imbalance, and achieve the effect of improving imaging sensitivity, reducing cost and improving sensitivity

Active Publication Date: 2021-06-15
XIDIAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0015] Aiming at the problems existing in the prior art, the present invention provides a high-sensitivity weak fluorescent signal detection system, method, storage medium and application, aiming at solving the gap between technical cost, imaging quality and imaging speed existing in the current weak light detection technology. balance problem

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  • High-sensitivity weak fluorescence signal detection system and method, storage medium and application

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Embodiment 1

[0086] see figure 2 , figure 2 It is an overall schematic diagram of a high-sensitivity weak fluorescent signal detection system provided by an embodiment of the present invention. A high-sensitivity weak fluorescent signal detection system provided by an embodiment of the present invention includes:

[0087] The front fluorescent signal collection module 1 uses a specially customized optical signal collection lens to receive the fluorescent signal from the sample, and filters out stray light signals and collects the fluorescent signal;

[0088] A multi-channel spatial encoding module 2, configured to perform multi-channel spatial encoding on the fluorescent signals collected by the front fluorescent signal collection module 1 and compress them into a multi-channel one-dimensional fluorescent signal array;

[0089] The array weak signal detection module 3 is used to receive a multi-channel one-dimensional fluorescent signal array and convert the multi-channel one-dimension...

Embodiment 2

[0092] see image 3 , Figure 4 and Figure 5 , image 3 It is a schematic structural diagram of a high-sensitivity weak fluorescent signal detection system provided by an embodiment of the present invention, Figure 4 is a schematic diagram of a single-channel spatial encoding process provided by an embodiment of the present invention, Figure 5 A schematic diagram of a transformation mode of four-channel spatial coding provided by an embodiment of the present invention.

[0093] The pre-fluorescence signal collection module 1 includes an optical signal collection lens 11 , a focusing lens 12 and a filter 13 , and the optical signal collection lens 11 , the focusing lens 12 and the filter 13 are sequentially arranged along the optical path of the system.

[0094] An optical signal collecting lens 11, configured to collect the fluorescent signal generated by the fluorescent sample and transmit the fluorescent signal to the focusing lens 12;

[0095] The optical signal col...

Embodiment 3

[0131] An application scenario of the high-sensitivity weak fluorescence signal detection method provided by the embodiment of the present invention is the fluorescence imaging scenario of small animals. In the small animal fluorescence imaging scene, the present invention can replace the traditional area detector CCD camera, greatly improve the detection sensitivity of the small animal fluorescence imaging technology to the small animal fluorescence signal, and at the same time reduce the cost of the CCD camera in the small animal fluorescence imaging technology. resulting price costs. The present invention can also be applied to other weak light imaging scenes with large field of view that require high sensitivity to weak light detection, including but not limited to small animal fluorescence imaging scenes, bioluminescence imaging scenes, Cerenkov fluorescence imaging scenes and Raman imaging scenes. Scattering imaging scenes, etc.; the main feature of the single-point dete...

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Abstract

The invention belongs to the technical field of biomedical imaging, and discloses a high-sensitivity weak fluorescence signal detection system and method, a storage medium and application. The high-sensitivity weak fluorescence signal detection system comprises a front fluorescence signal collection module for collecting a weak fluorescence signal, filtering a stray light signal and transmitting the fluorescence signal to a multi-channel space coding module in a blocked way, the multi-channel space coding module for performing block multi-channel space coding on the weak fluorescence signal transmitted by the front fluorescence signal collection module, an array weak signal detection module used for collecting a fluorescence signal coded by the block multi-channel space, converting the fluorescence signal into an electric signal and sending the electric signal to a control and calculation module, and the control and calculation module comprising a control unit and a calculation unit. According to the high-sensitivity weak fluorescence signal detection system, a multi-channel coding strategy is introduced; and in comparison with a space coding single pixel with fluorescence imaging technology, the number of collected fluorescence signals is reduced, and the collection time of weak fluorescence signals and the system imaging time are reduced.

Description

technical field [0001] The invention belongs to the technical field of biomedical imaging, and in particular relates to a high-sensitivity weak fluorescent signal detection system, method, storage medium and application. Background technique [0002] At present, weak light detection is an indispensable technology in biomedical optical imaging and detection, such as Raman spectral imaging technology and fluorescence imaging technology. Traditional fluorescence imaging techniques include planar imaging systems and point-scanning imaging systems; planar imaging systems mostly use CCD cameras with high sensitivity to collect fluorescence signals emitted by samples, which have the advantages of simple operation and large detection area. The sampling signal dimension of the device is large when performing weak signal light detection, the detection sensitivity is limited by the photosensitivity of the unit pixel, and the imaging sensitivity is low, and the ordinary CCD has a weak r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B5/00
CPCA61B5/0071A61B2503/40A61B2503/42
Inventor 陈雪利范大玮闫天宇曾琦谢晖徐欣怡陈多芳周王婷
Owner XIDIAN UNIV
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