High-sensitivity weak fluorescent signal detection system, method, storage medium and application

Abstract

The invention belongs to the technical field of biomedical imaging, and discloses a high-sensitivity weak fluorescent signal detection system, method, storage medium and application. The high-sensitivity weak fluorescent signal detection system includes: a front fluorescent signal collection module for collecting weak fluorescent signals signal, filter out stray light signals, and transmit the fluorescence signals in blocks to the multi-channel spatial encoding module; the multi-channel spatial encoding module is used to divide the weak fluorescent signals delivered by the pre-fluorescent signal collection module into multi-channel spaces Encoding; the array weak signal detection module is used to collect fluorescent signals encoded in block multi-channel space, and convert the fluorescent signals into electrical signals and send them to the control and calculation module; the control and calculation module includes a control unit and a calculation unit. The present invention introduces a multi-channel encoding strategy, which reduces the number of fluorescence signal acquisitions and reduces the acquisition time and system imaging time of weak fluorescence signals compared with the spatial encoding single pixel combined with fluorescence imaging technology.

Description

technical field [0001] The invention belongs to the technical field of biomedical imaging, and in particular relates to a high-sensitivity weak fluorescent signal detection system, method, storage medium and application. Background technique [0002] At present, weak light detection is an indispensable technology in biomedical optical imaging and detection, such as Raman spectroscopy imaging technology and fluorescence imaging technology. In the traditional fluorescence imaging technology, including plane imaging system and point scanning imaging system; most plane imaging systems use a highly sensitive CCD camera to collect the fluorescence signal emitted by the sample, which has the advantages of simple operation and large detection area. When the sensor detects weak signal light, the sampling signal dimension is large, the detection sensitivity is limited by the photosensitivity on the unit pixel, and the imaging sensitivity is low. When the number of photons on the unit ...

AI Technical Summary

Problems solved by technology

[0015] Aiming at the problems existing in the prior art, the present invention provides a high-sensitivity weak fluorescent signal detection system, method, storage medium and application, aiming at solving the gap between technical cost, imaging quality and imaging speed existing in the current weak light detection technology. balance problem

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