Method for constructing Alzheimer's disease (AD) cell model, and application of Alzheimer's disease (AD) cell model

Abstract

The invention discloses a method for constructing an Alzheimer's disease (AD) cell model, and application of the Alzheimer's disease (AD) cell model. The method comprises the following steps of: constructing a recombinant vector containing a coding gene of APP protein, and integrating the coding gene of the APP protein into a genome of a host cell by using the recombinant vector, so as to construct and form the cell model. According to the invention, CHO (Chinese Hamster Ovary) and HFF (Human Foreskin Fibroblast) cell lines overexpressing APP genes are constructed through a gene technology to serve as the AD cell model; a lentivirus transfection mode is adopted, so that it can be guaranteed that the AD cell model has the durability and stability of AD pathological characteristics; moreover, the AD cell model disclosed by the invention can stably express and secrete A beta, can be used for a long time, has stable and continuous A beta deposition and tau protein phosphorylation characteristics, and can well simulate pathological characteristics of the Alzheimer's disease; and an in-vitro cell research platform possibility is provided for research, development and screening of Alzheimer's disease related drugs and research of mechanisms.

Description

technical field [0001] The present invention relates to a method for constructing an Alzheimer's disease cell model, in particular to a method for constructing an Alzheimer's disease cell model (AD cellmodel) capable of secreting Aβ and Tau phosphorylation and its optimization , and its application in the detection of secreting Aβ and Tau phosphorylation ability in pathology in vitro, belonging to the technical field of biotechnology and genetic engineering. Background technique [0002] Alzheimer's disease (Alzheimer's Disease, hereinafter referred to as AD) is one of the most common and most destructive systemic degenerative diseases in the elderly. At present, it has two main pathological features: 1. Intracellular amyloid precursor protein is wrongly sheared to produce amyloid bata, and Aβ is secreted to the outside of the cell to form amyloid plaques; 2. Hyperphosphorylation of tau protein leads to neuronal hyperphosphorylation Adhesion between each other hinders nerve...

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Problems solved by technology

[0004] At this stage, AD cell models have been widely used, but there are still some shortcomings and disadvantages. The main problems are: (1) The ability of AD cell models to secrete Aβ and Tau phosphorylation is uneven, resulting in the degree of amyloid plaques and neurofibrillary tangles. Cannot be standardized; (2) Some AD cell models are mainly constructed by drug treatment, and the model is not stable enough to last for a long time; (3) Some AD cell models cannot simulate the pathological process of AD

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