Scanning type microscopic hyperspectral imaging system
A hyperspectral imaging and scanning technology, applied in fluorescence/phosphorescence, material analysis through optical means, measurement devices, etc., can solve the problem of low resolution of microscopic hyperspectral imaging, improve spatial resolution and eliminate blur and artifact effects
Pending Publication Date: 2021-06-18
SHENZHEN TECH UNIV
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[0005] An embodiment of the present invention provides a scanning microscopic hyperspectral imaging system, aiming to solve the technical problem of low resolution of existing microscopic hyperspectral imaging
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Embodiment 1
[0035] see Figure 1-Figure 2 , shows the scanning micro-hyperspectral imaging system in Embodiment 1 of the present invention, including an excitation light source 1 , a galvanometer scanning assembly 30 , a sample microscopic assembly 40 , an optical filter component 5 , and an image detection module 50 . in:
[0036] The excitation light source 1 is used to provide excitation light for illuminating the sample to be tested. The excitation light will be directed to the sample to be tested along the set exit light path. After the sample to be tested is excited by the excitation light, a fluorescent signal will be generated, and the fluorescent signal can be used for subsequent hyperspectral imaging. Preferably, the excitation light source 1 can specifically be a multi-threaded laser capable of emitting excitation light of various wavelengths, which can satisfy the excitation of various fluorescent signals in the range of visible light and improve the versatility of the system...
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The invention is suitable for the technical field of microscopic imaging, and provides a scanning type microscopic hyperspectral imaging system, which comprises an excitation light source; a sample microscopic assembly which comprises a sample table for placing a to-be-detected sample and an objective lens group for carrying out microscopy on the to-be-detected sample; a galvanometer scanning assembly which generates exciting light with different deflection angles, wherein the exciting light with different deflection angles is focused on the to-be-detected sample through the objective lens group so as to scan the to-be-detected sample; a light filtering component which is arranged on the exciting light emergent light path and the reflecting light path and is used for filtering the incident laser and the reflecting fluorescence; and an image detection module which is arranged on the reflecting light path and is used for receiving the filtered mixed fluorescence and carrying out microscopic hyperspectral imaging. According to the invention, the spatial resolution of the system is improved by utilizing a spot scanning imaging mode of light, high-resolution detection of various targets can be realized by combining different amplification factors of the objective lens group, and blurring and artifacts caused by mechanical movement of a sample to imaging are also eliminated.
Description
technical field [0001] The invention belongs to the technical field of microscopic imaging, in particular to a scanning microscopic hyperspectral imaging system. Background technique [0002] As a branch of spectral imaging technology, hyperspectral imaging has been widely used in emerging technologies in the fields of agriculture, mineralogy, atmospheric and life sciences since its birth in the 1980s. Compared with the traditional imaging technology, it can obtain the spectral information of a specific band while obtaining the target image information, and the spectral resolution of hyperspectral imaging can reach below 10nm, and it can instantaneously record every pixel in the field of view Spectral information of dozens or even hundreds of continuous narrow bands. In recent years, with the penetration of microscopic technology into all aspects of biomedical testing, there have been many reports on the combination of hyperspectral technology and microscopic imaging, such ...
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CPCG01N21/6458G01N21/6402G01N21/64G01N21/645G01N2021/6417G01N2021/6471
Inventor 胡学娟陈玲玲黄圳鸿李贵叶徐露贺婷张家铭胡凯
Owner SHENZHEN TECH UNIV