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Tissue culture rapid propagation method of Vriesea pineapples

A tissue culture and pineapple technology, applied in the field of plant seedling raising, can solve the problem of high variation rate of pineapple tissue culture, and achieve the effects of solving large-scale production, efficient acquisition method and improved efficiency

Inactive Publication Date: 2021-06-25
苏州回文生物种业科技有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

After verification of a large number of products, the use of this series of medium formulas effectively solves the problem of high variation rate in pineapple tissue culture

Method used

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  • Tissue culture rapid propagation method of Vriesea pineapples
  • Tissue culture rapid propagation method of Vriesea pineapples
  • Tissue culture rapid propagation method of Vriesea pineapples

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Embodiment Construction

[0037] Reagent abbreviations: 6-BA is 6-benzylaminopurine; NAA is naphthaleneacetic acid; IBA is 3-indolebutyric acid; IAA is indole-3-acetic acid.

[0038] The tissue culture rapid propagation method (recorded as method A) of Yingge Pineapple comprises the following steps:

[0039] 1. Preparation of sterile explants: use the old leaf sheaths of plants as explants, wash the explants of Pineapple genus Yingge with water for 5 minutes, cut them into small pieces with bud points; after cleaning, use 70V % ethanol aqueous solution soaked for 1min, transferred to the ultra-clean workbench, then soaked in 3wt% sodium hypochlorite aqueous solution for 10min, and finally soaked 3 times with 0.15wt% citric acid water, and dried to obtain sterile explants.

[0040] 2. Preparation of aseptic seedlings: Inoculate aseptic pineapple explants on the start-up medium, at a temperature of 22-28°C, under a light intensity of 1800-2200lux, cultivate in the dark for 12 hours after light cultivatio...

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Abstract

The invention belongs to the technical field of plant seedling raising, and relates to a method for tissue culture rapid propagation of Vriesea pineapples. The method comprises the following steps: 1) preparation of a sterile explant: cleaning Vriesea pineapple explants with water, and then carrying out disinfection to obtain sterile explants; 2) preparation of sterile seedlings: inoculating a starting culture medium with the sterile explants, and performing culturing to obtain sterile seedlings; 3) preparation of tissue culture seedlings: inoculating a proliferation culture medium with the sterile seedlings, and performing culturing to obtain tissue culture seedlings; and (4) preparation of rooting seedlings and tissue culture seedlings: dividing cluster buds of the obtained tissue culture seedlings, selecting plants with the cluster buds greater than or equal to 1.5 cm, and inoculating a rooting culture medium with the plants to obtain tissue culture rooting seedlings.

Description

technical field [0001] The invention belongs to the technical field of plant seedling cultivation, and relates to a method for tissue culture and rapid propagation of pineapple of the genus Yingge. Background technique [0002] Ornamental pineapple is a perennial herbaceous plant of Bromeliaceae. It is native to tropical and subtropical regions of Central and South America. It is mainly epiphytic species, usually epiphytic on tree trunks or stone walls, and prefers warm and humid semi-shade environments. [0003] The common species and varieties are mainly Aech-mea, Billbergia, Guzmania, Neo-regelia and Tillandsia belonging to Bromeliaceae. ) and the genus Vriesea. They are mainly for viewing flowers, and there are also types for viewing leaves. Among them, there are many types of flowers and leaves that look both flowers and leaves. Yingge pineapple has straight and slender flower stems, bright bracts, gorgeous red and yellow colors, exquisite and cute, and can purify ind...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 尹翠翠张文杰冷江明郭俊清付舒滔
Owner 苏州回文生物种业科技有限公司