Method for identifying cyclocarya paliurus

A technology of Cyclocarya paliurus and powder, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of many counterfeit products of Cyclocarya paliurus, lack of good identification methods, and unfavorable development of the value of Cyclocarya paliurus, and achieve rapid detection and evaluation, fast response, and simple operation

Inactive Publication Date: 2021-06-25
轩豪益邦制药有限公司
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Problems solved by technology

At present, the amount of Cyclocarya paliurus is increasing, but there is no good identification method so far, so there are many counterfeit and inf

Method used

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  • Method for identifying cyclocarya paliurus

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Embodiment

[0018] A method for identifying Cyclocarya paliurus, comprising the steps of:

[0019] (1) Take 1g of the powder to be tested, add the powder to 50mL of methanol solution (the mass percentage of methanol is 60%, and the rest is water), soak at room temperature for 30min, and ultrasonically treat for 1h after soaking, the ultrasonic power is 400W, and the frequency is 20kHz;

[0020] (2) The solution after the ultrasonic treatment is filtered to obtain the filtrate, and the filtrate is evaporated to dryness in a water bath to obtain a once-evaporated product, and the once-evaporated product is added with 30 mL of water to dissolve and form an aqueous solution, and the hydrochloric acid (solute mass fraction) of an equal volume of the aqueous solution is added to the aqueous solution. 10%, the rest is water) to obtain a mixed solution, the mixed solution is heated and boiled, condensed and refluxed for 1h, after the heating is completed, air-cooled to room temperature, extracted...

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Abstract

The invention discloses a method for identifying cyclocarya paliurus. The method comprises the following steps: (1) preparing a test solution; and (3) respectively preparing a methanol solution of kaempferide and a methanol solution of quercetin as reference substance solutions, respectively dripping the two reference substance solutions and the test solution on the same silica gel G thin-layer plate, developing by using dichloromethane-ethyl acetate-formic acid as a developing solvent, taking out, airing, spraying an ethanol solution of aluminum trichloride, heating the sample solution in the environment of 105 +/-5 DEG C until the spots are clearly developed, placing the sample solution under an ultraviolet lamp for inspection, and when the fluorescent spots in the chromatogram of the sample solution can find the corresponding fluorescent spots in the corresponding position of the chromatogram of one of the two reference substance solutions, determining that the powder to be detected is cyclocarya paliurus powder. The method is high in cyclocarya paliurus identification accuracy, meanwhile, the content of kaempferide in cyclocarya paliurus can be measured, the quality grade of cyclocarya paliurus can be evaluated, operation is easy, reaction is fast, and rapid detection and evaluation of the quality of cyclocarya paliurus are achieved.

Description

technical field [0001] The invention belongs to the technical field of identification of Cyclocarya paliurus beverages, and in particular relates to an identification method of Cyclocarya paliurus. Background technique [0002] Cyclocarya is a single-species plant of the Juglandaceae Cyclocarya genus, also known as sweet tea tree, money tree, green money plum, a string of money, mountain tree, camellia, and mountain hemp tree. Cyclocarya paliurus is scattered in the mountains, valleys or limestone mountains of Jiangxi, Jiangsu, Guangxi, Fujian, Zhejiang and other places in China. Residents in many places in Jiangxi often use its leaves as tea, believing that the leaves are sweet and moist, and have the effects of promoting body fluids and quenching thirst, clearing away heat and detoxification, and lowering blood pressure. At present, the amount of Cyclocarya paliurus is increasing, but there is no good identification method so far, so there are many counterfeit and inferio...

Claims

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Application Information

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IPC IPC(8): G01N30/90G01N30/02
CPCG01N30/90G01N30/02
Inventor 戴龙勇王文勇
Owner 轩豪益邦制药有限公司
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