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A kind of construction method and application of mycoplasma bovis mouse mastitis model

A technology of Mycoplasma bovis and Mycoplasma bovis, which is applied in the field of constructing a mouse model of Mycoplasma bovis, can solve problems such as the lack of pathological damage evaluation models, obstacles in the research and development of antibiotic alternative drugs for anti-inflammatory treatment programs for dairy cow mastitis, and achieve good results. Practical application value, good repeatability and convenience, and the effect of promoting development

Active Publication Date: 2022-06-21
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for cow mastitis caused by Mycoplasma bovis, there is still a lack of an effective pathological damage evaluation model
The structural specificity of the blood-milk barrier makes the treatment of mycoplasma mastitis different from the model of bovine mycoplasma pneumonia, which brings obstacles to the anti-inflammatory treatment of dairy cow mastitis and the development of antibiotic alternatives

Method used

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  • A kind of construction method and application of mycoplasma bovis mouse mastitis model
  • A kind of construction method and application of mycoplasma bovis mouse mastitis model
  • A kind of construction method and application of mycoplasma bovis mouse mastitis model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1. Selection of strains for infection

[0047] Mycoplasma bovis NMH7 strain is a Mycoplasma bovis isolate isolated by the inventor's research group in 2018 from a milk sample from a dairy farm in Inner Mongolia. After epidemiological and pathogenic analysis, a large amount of background information was obtained on the genotype characteristics and pathogenicity of M. bovis NMH7 strains.

[0048] The results of multi-locus sequence analysis showed that the M. bovis NMH7 strain belongs to a newly discovered genotype in my country, ST173, and its background information is included in the MLST database (https: / / pubmlst.org / bigsdb?db=pubmlst_mbovis_isolates&page=query) (details See doi: 10.1016 / j.prevetmed.2020.105106). Mycoplasma bovis NMH7 strain is documented at: https: / / pubmlst.org / bigsdb?id=2 db=pubmlst_mbovis_isolates&page=query, the ID number is 1015.

[0049]Further, the inventor tested milk samples from dairy farms across the country and found that the geno...

Embodiment 2

[0052] Example 2, Mycoplasma bovis infection causes mastitis in mice

[0053] 1. Construction of mastitis model mice

[0054] The infectious fluid was inoculated into lactating CD-1 mice 5 days after delivery. The method of inoculation is through the milk duct of mice. The inoculation site was the fourth pair of mammary glands of mice. The infection solution was obtained by suspending the Mycoplasma bovis NMH7 strain with sterile phosphate buffer solution, and the concentration of Mycoplasma bovis was 1 × 10 6 CFU / ml. The inoculation volume was 100 μl of infection solution per mammary gland.

[0055] 2. Control mice

[0056] Sterile phosphate buffered solution was inoculated into lactating CD-1 mice 5 days after delivery. The method of inoculation is through the milk duct of mice. The inoculation site was the fourth pair of mammary glands of mice. The inoculum volume was 100 μl sterile phosphate buffer solution per mammary gland.

[0057] 3. Detection of relevant indi...

Embodiment 3

[0072] Example 3. Experiment on the protective effect of reactive oxygen species scavengers on mouse mastitis caused by Mycoplasma bovis infection

[0073] NAC (N-acetyl-L-cysteine): a reactive oxygen species scavenger, Sigma Company, USA, product number A7250.

[0074] NAC was diluted with sterile physiological saline to obtain a NAC solution with a concentration of 10 mM.

[0075] 1. Treatment of M.bovis group + NAC group

[0076] 1. Take lactating CD-1 mice 5 days after delivery, and inject NAC solution (150 mg NAC / kg body weight) intraperitoneally.

[0077] 2. Inoculate the infection solution 12 hours after completing the intraperitoneal injection. The method of inoculation is through the milk duct of mice. The inoculation site was the fourth pair of mammary glands of mice. The infection solution was obtained by suspending the Mycoplasma bovis NMH7 strain with sterile phosphate buffer solution, and the concentration of Mycoplasma bovis was 1 × 10 6 CFU / ml. The inocul...

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Abstract

The invention discloses a construction method and application of a mycoplasma bovis mouse mastitis model. The invention provides a method for constructing a mouse mastitis model, comprising the following steps: inoculating an infection solution into the mammary gland of a lactating mouse; the infection solution is obtained by suspending the Mycoplasma bovis NMH7 strain with a sterile buffer solution. Specifically, the inoculation method can be inoculation through the milk duct of mice. Specifically, the mammary gland of the mouse is the fourth pair of mammary glands of the mouse. The present invention also protects the application of N-acetyl-L-cysteine ​​in the preparation of medicine for cow mastitis caused by Mycoplasma bovis. The model constructed by the invention can simulate the clinical pathological changes of cow mammary glands when Mycoplasma bovis cow mastitis occurs, has good repeatability and convenience, and has good practical application value.

Description

technical field [0001] The invention belongs to the technical field of animal model construction, and in particular relates to a construction method and application of a Mycoplasma bovis murine mastitis model. Background technique [0002] Mycoplasma bovis (M.bovis) can cause mastitis, pneumonia, arthritis and otitis media in cattle. Dairy cow mastitis is an inflammatory reaction of red, swollen, hot, and painful mammary glands caused by pathogenic microorganism infection or other physical and chemical stimuli. Mycoplasma bovis mastitis in dairy cows can cause systemic symptoms such as fever in dairy cows, and inflammatory reactions such as redness, swelling, heat and pain may occur locally in the milk area. Mycoplasma bovis mastitis in dairy cows can lead to a sharp drop in milk production or stop milk production, reduce the content of fat or protein in milk, and reduce milk quality, which seriously affects the production performance of dairy cows. The mammary glands of i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/027A61K49/00A61K31/198A61P15/14A61P31/04
CPCA01K67/027A61K49/0008A61K31/198A61P15/14A61P31/04A01K2207/20A01K2227/105A01K2267/03
Inventor 韩博刘洋高健王登峰石玉祥
Owner CHINA AGRI UNIV
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