Salt-tolerant potassium-solubilizing growth-promoting microbacterium oxydans 41-C8, microbial agent and application thereof

A microbacterium oxidase, 41-C8 technology, applied in the field of microorganisms, can solve the problems of lack of high salt-tolerant growth-promoting microorganisms, lack of salt-tolerant growth-promoting microorganisms, etc., to improve salt-tolerant growth-promoting ability, slow down the phenomenon of salt damage, improve The effect of salt tolerance

Active Publication Date: 2021-06-29
QINGDAO AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there are few studies on salt-tolerant growth-promoting microorganisms, and there is a lack of salt-tolerant growth-promoting microorganisms, especially the lack of effective high-salt-tolerant growth-promoting microorganisms

Method used

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  • Salt-tolerant potassium-solubilizing growth-promoting microbacterium oxydans 41-C8, microbial agent and application thereof
  • Salt-tolerant potassium-solubilizing growth-promoting microbacterium oxydans 41-C8, microbial agent and application thereof
  • Salt-tolerant potassium-solubilizing growth-promoting microbacterium oxydans 41-C8, microbial agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Isolation and Screening of Example 1 Bacterial Strain 41-C8

[0049] Soil samples were collected from saline-alkali soil in Dongying City, and different microorganisms were isolated from them, and the strains were streaked on a plate and stored on a solid LB medium plate for use. Pick the colonies and put them in the liquid LB medium Erlenmeyer flasks with different NaCl concentrations of 0.1%, 0.3%, 0.5% and 0.7% respectively for shaking flask culture, and cultivate them at 27℃~29℃120r / min for 2~ On the 3rd day, the production of the strains under different salt concentrations was observed, and the screened strains with good growth were repeatedly streaked and purified on plates to obtain five salt-tolerant strains with a single colony, which was named strain 41-C8.

Embodiment 2

[0050] Functional Qualitative Identification of Example 2 Bacterial Strain 41-C8

[0051] Prior to the experiment in this example, strain 41-C8 was cultured in shake flasks, activated and multiplied, respectively prepared potassium-dissolving, phosphorus-dissolving and nitrogen-fixing solid medium, and prepared a sterilized plate, and poured the plate after the medium was sterilized. After solidification, pipette 1 μL of the bacterial solution onto the plate, seal the plate and place it upside down at 27°C for culture. When there is a transparent hydrolysis circle or plaque, observe the experimental results. Such as figure 2 As shown, the strain 41-C8 of this example can dissolve organic phosphorus, poorly soluble potassium and fix nitrogen. Organic phosphorus medium, inorganic phosphorus medium, potassium solution medium and nitrogen fixation medium are as follows.

[0052] Organic phosphorus medium: glucose 10g, NaCl 0.3g, MgSO 4 0.3g, MnSO 4 0.03g, CaCO 3 5g, lecit...

Embodiment 3

[0058] The potassium decomposing property of the bacterium liquid, thalline and supernatant of embodiment 3 bacterial strains 41-C8

[0059] Table 1 Potassium-dissolving analysis of bacterial strain 41-C8

[0060] different treatment Colony diameter (d) / cm Hydrolysis circle diameter (D) / cm Potassium solubility index (D / d) Bacteria 0.9 1.5 1.67 bacteria 0.8 1 1.25 Supernatant 0.7 0.9 1.29 Sterilization solution - - -

[0061] Prepare the potassium-dissolving solid medium plate in advance, conduct further potassium-dissolving analysis on the strain 41-C8, culture the strain 41-C8 in a shake flask, draw the bacterial liquid into a centrifuge tube, centrifuge at 5000r / min for two minutes, and centrifuge Then pipette the supernatant into a new centrifuge tube, and resuspend the remaining bacteria in sterile water. Then pipette 1 μL of the bacterial solution, supernatant, sterilized bacterial solution and bacterial suspension into...

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Abstract

The invention provides a salt-tolerant potassium-solubilizing growth-promoting microbacterium oxydans 41-C8, which is preserved in China General Microbiological Culture Collection Center on January 4, 2021, and has a preservation registration number of CGMCC No.21585. The bacterium can be applied to potassium solubilizing, nitrogen fixation, phosphorus solubilizing and plant growth promotion, and can be used as a salt-tolerant potassium-solubilizing growth-promoting microbacterium oxydans 41-C8. The invention further provides a microbial agent containing the microbacterium oxydans 41-C8 and a preparation method of the microbial agent. The strain has the salt tolerance of 0-7% and the optimal salt tolerance of 3-5%, and has various growth promoting characteristics of nitrogen fixation, organophosphorus and potassium decomposition and the like, and a bacterial solution prepared from the microbacterium oxydans 41-C8 can significantly improve the salt tolerance and growth promoting ability of Chinese cabbages, alleviate the salt damage phenomenon and increase the biomass under salt stress, so that the growth effect of the Chinese cabbages is promoted, the salt tolerance of the Chinese cabbages is improved, and the use of chemical fertilizers is reduced, and the salt-tolerant growth-promoting bacterium strain and the bacterium liquid thereof have wide application prospects.

Description

technical field [0001] The invention belongs to the field of microbes, and in particular relates to a salt-resistant, potassium-stimulating growth-promoting microbacterium oxidase 41-C8, a bacterial agent and applications thereof. Background technique [0002] The total area of ​​saline-alkali land in my country is 99.13 million hm 2 , accounting for about 10% of the total area of ​​saline-alkali land in the world. Soil salinization has brought great obstacles to agricultural production. Salt stress can easily lead to crop water loss, ion imbalance, nutrient deficiency, etc. It is still one of the main factors affecting crop yield and quality in the world, and it is easy to cause crop Metabolic disorders such as the imbalance of active oxygen generation and removal, photosynthesis decline, etc., lead to a substantial reduction in yield and quality of facility crops. Salt stress affects the nutrient absorption of plants, produces osmotic stress, reduces the photosynthesis o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/20A01P21/00C12R1/01
CPCC12N1/20A01N63/20
Inventor 刘新刘广超郭艳苹叶青白洋张婧赢李雅华
Owner QINGDAO AGRI UNIV
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