Bio-valve material modified by zwitterionic polymer and preparation method thereof

A zwitterion, biological valve technology, used in drug delivery, tissue regeneration, pharmaceutical formulations, etc., to reduce biological toxicity problems, reduce coagulation risks, and reduce calcification problems.

Inactive Publication Date: 2021-07-09
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the deficiencies or defects in the above-mentioned prior art, the object of the present invention is to provide a zwitterionic polymer modified biological valve material and its preparation method, aiming at solving the problems of calcification and blood coagulation in the existing biological valve, prolonging the life of the biological valve service life

Method used

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  • Bio-valve material modified by zwitterionic polymer and preparation method thereof
  • Bio-valve material modified by zwitterionic polymer and preparation method thereof
  • Bio-valve material modified by zwitterionic polymer and preparation method thereof

Examples

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Embodiment 1

[0034] This embodiment 1 provides a preparation method of a zwitterionic polymer-modified biological valve material, which specifically includes the following steps:

[0035] (1) fresh porcine pericardium, remove connective tissue and carry out cleaning process, further clean up with distilled water;

[0036] (2) Soak the porcine pericardium in step (1) in an aqueous zwitterionic polymer solution (50mg / mL) for 3 hours, add 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide Hydrochloride (50mg / mL) and N-hydroxysuccinimide (30mg / mL), react at 25°C and pH 8.0 for 24 hours, take it out and wash it;

[0037] (3) Soak the porcine pericardium in step (2) in a glutaraldehyde solution with a mass concentration of 1%, perform cross-linking treatment at 25° C. for 24 hours, and clean it with distilled water after taking it out;

[0038] (4) The porcine pericardium was further treated with 5% aminoglycerin for 24 hours, and then removed and cleaned with distilled water.

[0039] The preparat...

Embodiment 2

[0044] This embodiment 2 provides a preparation method of a zwitterionic polymer-modified biological valve material, which specifically includes the following steps:

[0045] (1) fresh porcine pericardium, remove connective tissue and carry out cleaning process, further clean up with distilled water;

[0046] (2) Soak the porcine pericardium of step (1) in the zwitterionic polymer aqueous solution (50mg / mL) for 3 hours, then add glutaraldehyde solution, the final mass concentration of glutaraldehyde is 1%, at 4 ℃ , under the condition of pH 2.0, continue to soak for 24 hours, then take it out and clean it with distilled water;

[0047] (3) The porcine pericardium was further treated with 5% aminoglycerin for 24 hours, and then removed and cleaned with distilled water.

[0048] The preparation process of above-mentioned zwitterionic polymer is as follows:

[0049]

[0050] Step 1: 3-[N,N-Dimethyl-[2-(2-methylprop-2-enoyloxy)ethyl]ammonium]propane-1-sulfonic acid inner salt...

Embodiment 3

[0053] This embodiment 3 provides a preparation method of a zwitterionic polymer-modified biological valve material, which specifically includes the following steps:

[0054] A preparation method of a biovalve material modified by a zwitterionic polymer, comprising the following steps:

[0055] (1) fresh porcine pericardium, remove connective tissue and carry out cleaning process, further clean up with distilled water;

[0056] (2) Soak the porcine pericardium in step (1) in a glutaraldehyde solution with a mass concentration of 1%, cross-link for 24 hours at 4°C and pH=7.0, then take it out and clean it with distilled water;

[0057] (3) Soak the glutaraldehyde-crosslinked porcine pericardium in an aqueous zwitterionic polymer solution (50 mg / mL) for 6 hours, add 1-(3-dimethylaminopropyl)-3-ethylcarbodiethylene Amine hydrochloride (50mg / mL) and N-hydroxysuccinimide (30mg / mL), react at 25°C, pH 5.0 for 24 hours, take it out and wash it;

[0058] (4) The porcine pericardium w...

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Abstract

The invention discloses a bio-valve material modified by a zwitterionic polymer and a preparation method thereof, and belongs to the technical field of bio-valve material preparation, the method comprises the following steps: synthesizing a polymer with amino and zwitterions, and connecting the obtained polymer and an acellular bioprosthetic valve to the bio-valve in a chemical bonding manner. The bio-valve material prepared by the invention is a bio-valve material modified by a zwitterionic polymer, and the zwitterionic polymer is added before glutaraldehyde crosslinking or after glutaraldehyde crosslinking. The bio-valve material prepared by the invention not only can solve the problems of calcification and blood coagulation in the existing bio-valve, but also can prolong the service life of the bio-valve.

Description

technical field [0001] The invention relates to the technical field of biological valve material preparation, in particular to a zwitterionic polymer modified biological valve material and a preparation method thereof. Background technique [0002] Traditional surgical thoracotomy is an important way to treat valvular diseases, but it has high surgical risks, large trauma, and long recovery time for patients. In recent years, transcatheter minimally invasive interventional replacement of diseased heart valves has become the main treatment for valve diseases. However, minimally invasive interventional valve replacement surgery often uses glutaraldehyde-crosslinked biological valves as valve leaflets, which are prone to calcification and coagulation problems, and pure glutaraldehyde-crosslinked biological valves have poor durability and need to be implanted after a certain number of years. Replace again. Therefore, how to further modify the glutaraldehyde-crosslinked biologi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/50C08F220/60C08F230/02C08F8/00C08F220/38
CPCA61L27/3604A61L27/3687A61L27/50C08F220/603C08F8/00A61L2430/20A61L2430/40A61L2400/18C08F230/02C08F220/387
Inventor 陈茂冯沅庄伟华李淑芬
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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