Bio-valve material modified by zwitterionic polymer and preparation method thereof
A zwitterion, biological valve technology, used in drug delivery, tissue regeneration, pharmaceutical formulations, etc., to reduce biological toxicity problems, reduce coagulation risks, and reduce calcification problems.
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Embodiment 1
[0034] This embodiment 1 provides a preparation method of a zwitterionic polymer-modified biological valve material, which specifically includes the following steps:
[0035] (1) fresh porcine pericardium, remove connective tissue and carry out cleaning process, further clean up with distilled water;
[0036] (2) Soak the porcine pericardium in step (1) in an aqueous zwitterionic polymer solution (50mg / mL) for 3 hours, add 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide Hydrochloride (50mg / mL) and N-hydroxysuccinimide (30mg / mL), react at 25°C and pH 8.0 for 24 hours, take it out and wash it;
[0037] (3) Soak the porcine pericardium in step (2) in a glutaraldehyde solution with a mass concentration of 1%, perform cross-linking treatment at 25° C. for 24 hours, and clean it with distilled water after taking it out;
[0038] (4) The porcine pericardium was further treated with 5% aminoglycerin for 24 hours, and then removed and cleaned with distilled water.
[0039] The preparat...
Embodiment 2
[0044] This embodiment 2 provides a preparation method of a zwitterionic polymer-modified biological valve material, which specifically includes the following steps:
[0045] (1) fresh porcine pericardium, remove connective tissue and carry out cleaning process, further clean up with distilled water;
[0046] (2) Soak the porcine pericardium of step (1) in the zwitterionic polymer aqueous solution (50mg / mL) for 3 hours, then add glutaraldehyde solution, the final mass concentration of glutaraldehyde is 1%, at 4 ℃ , under the condition of pH 2.0, continue to soak for 24 hours, then take it out and clean it with distilled water;
[0047] (3) The porcine pericardium was further treated with 5% aminoglycerin for 24 hours, and then removed and cleaned with distilled water.
[0048] The preparation process of above-mentioned zwitterionic polymer is as follows:
[0049]
[0050] Step 1: 3-[N,N-Dimethyl-[2-(2-methylprop-2-enoyloxy)ethyl]ammonium]propane-1-sulfonic acid inner salt...
Embodiment 3
[0053] This embodiment 3 provides a preparation method of a zwitterionic polymer-modified biological valve material, which specifically includes the following steps:
[0054] A preparation method of a biovalve material modified by a zwitterionic polymer, comprising the following steps:
[0055] (1) fresh porcine pericardium, remove connective tissue and carry out cleaning process, further clean up with distilled water;
[0056] (2) Soak the porcine pericardium in step (1) in a glutaraldehyde solution with a mass concentration of 1%, cross-link for 24 hours at 4°C and pH=7.0, then take it out and clean it with distilled water;
[0057] (3) Soak the glutaraldehyde-crosslinked porcine pericardium in an aqueous zwitterionic polymer solution (50 mg / mL) for 6 hours, add 1-(3-dimethylaminopropyl)-3-ethylcarbodiethylene Amine hydrochloride (50mg / mL) and N-hydroxysuccinimide (30mg / mL), react at 25°C, pH 5.0 for 24 hours, take it out and wash it;
[0058] (4) The porcine pericardium w...
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