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Composite sodium hyaluronate gel capable of resisting hyaluronidase hydrolysis and preparation method of composite sodium hyaluronate gel

A technology of hyaluronidase and sodium hyaluronate, used in pharmaceutical formulations, medical science, prostheses, etc., can solve the problems of reduced biocompatibility, easy to cause immune response, etc., to delay degradation time, strengthen anti-enzyme Degradation ability, the effect of inhibiting enzymatic degradation

Active Publication Date: 2021-07-09
QINGDAO CHENLAND MARINE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The degree of cross-linking affects the properties of fillers. Increasing the degree of cross-linking is the main way to prolong the degradation time. The method of adding cross-linking agents is often used. Any residue of cross-linking agents cannot be completely removed under the existing technology. Cross-linking Excessive dosage of the combination will lead to a decrease in biocompatibility, and it is easy to cause an immune response when injected into the human body

Method used

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  • Composite sodium hyaluronate gel capable of resisting hyaluronidase hydrolysis and preparation method of composite sodium hyaluronate gel
  • Composite sodium hyaluronate gel capable of resisting hyaluronidase hydrolysis and preparation method of composite sodium hyaluronate gel
  • Composite sodium hyaluronate gel capable of resisting hyaluronidase hydrolysis and preparation method of composite sodium hyaluronate gel

Examples

Experimental program
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Effect test

Embodiment 1

[0029] A chondroitin sulfate-sodium hyaluronate composite gel resistant to hyaluronidase hydrolysis, specifically prepared by the following method:

[0030] Weigh 1g of chondroitin sulfate (molecular weight: 10kDa), dissolve it in 50mL of NaOH solution (pH = 11) and keep stirring, add 5g of sodium hyaluronate dry powder (molecular weight: 800kDa), then add 1mL of BDDE and stir evenly. React for 4 hours in an environment with a temperature of 37° C., and obtain a primary cross-linked gel after the reaction is completed. The gel was precipitated with 95% ethanol, and the precipitate was repeatedly washed with purified water and then dried to obtain a gel powder.

[0031] Weigh 10g sodium hyaluronate dry powder (molecular weight 1500kDa) and dissolve it in 50mL NaOH solution (pH=11), then add 2g primary cross-linked gel dry powder and stir evenly, and stir and react at 37°C for 5h. After the reaction was completed, 1 mL of BDDE was added and stirred evenly to carry out secondary...

Embodiment 2

[0034] A chondroitin sulfate-sodium hyaluronate composite gel resistant to hyaluronidase hydrolysis, specifically prepared by the following method:

[0035] Weigh 2g of chondroitin sulfate (molecular weight: 10kDa), dissolve it in 50mL of NaOH solution (pH=11) and keep stirring, add 5g of sodium hyaluronate dry powder (molecular weight: 800kDa), add 1mL of BDDE and stir evenly. React for 4 hours in an environment with a temperature of 37° C., and obtain a primary cross-linked gel after the reaction is completed. The gel was precipitated with 95% ethanol, and the precipitate was repeatedly washed with purified water and then dried to obtain a gel powder.

[0036] Weigh 10g sodium hyaluronate dry powder (molecular weight 1500kDa) and dissolve it in 50mL NaOH solution (pH=11), then add 2g primary cross-linked gel dry powder and stir evenly, and stir and react at 37°C for 5h. After the reaction was completed, 1 mL of BDDE was added and stirred evenly to carry out secondary cross-li...

Embodiment 3

[0038] A chondroitin sulfate-sodium hyaluronate composite gel resistant to hyaluronidase hydrolysis, specifically prepared by the following method:

[0039] Weigh 2g of chondroitin sulfate (molecular weight: 10kDa), dissolve it in 50mL of NaOH solution (pH=11) and keep stirring, add 5g of sodium hyaluronate dry powder (molecular weight: 800kDa), add 1mL of BDDE and stir evenly. React for 4 hours in an environment with a temperature of 37° C., and obtain a primary cross-linked gel after the reaction is completed. The gel was precipitated with 95% ethanol, and the precipitate was repeatedly washed with purified water and then dried to obtain a gel powder.

[0040] Weigh 10g sodium hyaluronate dry powder (molecular weight 1500kDa) and dissolve it in 50mL NaOH solution (pH=11), then add 2g primary cross-linked gel dry powder and stir evenly, and stir and react at 37°C for 5h. After the reaction was completed, 1 mL of BDDE was added and stirred evenly to carry out secondary cross-...

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Abstract

The invention discloses composite sodium hyaluronate gel capable of resisting hyaluronidase hydrolysis and a preparation method of the composite sodium hyaluronate gel, and belongs to the technical field of preparation of the composite sodium hyaluronate gel. The sodium hyaluronate gel provided by the invention has two modes for improving the enzymatic hydrolysis resistance: 1, the sodium hyaluronate gel contains an active functional group which can be combined into a sodium hyaluronate structure in a grafting mode; and secondly, the sodium hyaluronate gel is added into the sodium hyaluronate gel in a direct adding manner. The hyaluronidase activity inhibitor and sodium hyaluronate construct a gel system, the gel degradation time can be delayed under the condition of not excessively adding a cross-linking agent, the in-vivo degradation time of the gel is prolonged by inhibiting the hyaluronidase activity, and a new thought is provided for facial injection of gel functional materials.

Description

technical field [0001] The invention belongs to the technical field of compound sodium hyaluronate gel preparation, and in particular relates to a compound sodium hyaluronate gel resistant to hyaluronidase hydrolysis and a preparation method thereof. Background technique [0002] Sodium hyaluronate is a natural macromolecular polysaccharide that exists in various organs and tissues of higher animals, and is an important component of human interstitial cells, synovial fluid and other tissues. It is composed of glucuronic acid and N-acetylglucosamine, has good biocompatibility, high viscoelasticity and unique molecular structure and physical and chemical properties, and shows a variety of important physiological functions in the body. [0003] Injectable fillers are one of the most effective ways to treat facial aging wrinkles and depressions at this stage, and sodium hyaluronate products in injectable fillers are relatively stable products. Sodium hyaluronate can be used in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/24C08L5/08A61L27/44A61L27/50A61L27/54
CPCC08J3/243A61L27/50A61L27/44A61L27/54C08J2305/08C08J2405/08A61L2400/06C08L5/08
Inventor 林莎莎邹圣灿王宝群姜晓文王君廷
Owner QINGDAO CHENLAND MARINE BIOTECH CO LTD
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