Preparation method and application of sensing electrode for glucose detection
A technology for sensing electrodes and glucose, applied in the field of glucose detection, can solve the problems of low detection sensitivity, unresearched influence of glucose interference, glucose detection interference, etc., and achieves the effects of high detection sensitivity, low price, and strong anti-interference ability.
Inactive Publication Date: 2021-07-09
李荣毓
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Problems solved by technology
First of all, the conductivity of ZIF-67 material is lower than that of bare glassy carbon electrodes, the detection sensitivity of this sensor material is very low, and the influence of the coexistence of similar components on glucose interference has not been studied
However, the detection of glucose is easily interfered by coexisting substances or structural analogues.
Method used
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Embodiment 1
[0020] (1) Preparation of sensor electrodes
[0021] Disperse 0.06g of aminated carbon nanotubes (CNT) in 20mL of N,N-dimethylformamide (DMF) solution, add 1.2mmol of cobalt nitrate, dissolve 1.53g of dimethylimidazole in 50mL of methanol solution and add Solution, react at room temperature for 24h. The obtained product was collected by centrifugation and washed with methanol. Dry in an oven at 80° C. for 12 hours to obtain a ZIF-67 / CNT composite material.
[0022] The prepared ZIF-67 / CNT composite material was dispersed in N,N-dimethylformamide (DMF) solution to form a 1 mg / mL dispersion. Take a certain amount (about 6 μL) and drop-coat it on the polished glassy carbon electrode, and let it dry naturally to modify the electrode.
[0023] The modified electrode prepared above was placed in 0.1 mol / L boric acid-acetic acid-phosphate buffer solution (pH=2) containing glucose and p-phenylenediamine (molar ratio 1:1), at 0.02V s -1 The scan rate was 20 cycles of cyclic voltamm...
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The invention discloses a preparation method and application of a sensing electrode for glucose detection, and belongs to the field of glucose detection. In order to solve the problems that a sensor material for glucose detection is low in detection sensitivity and high in detection condition requirement, glucose detection is easily interfered by coexisting substances or structural analogues and the like, a ZIF-67 / CNT composite material is prepared firstly, then a glassy carbon electrode is modified with dispersion liquid of the composite material, and the modified electrode is obtained. The modified electrode is placed in a boric acid-acetic acid-phosphoric acid buffer solution containing glucose and p-phenylenediamine, cyclic voltammetry scanning is carried out in a set potential interval, the electrode is taken out and naturally placed to be dry, and after liquid elution, the molecularly imprinted sensing electrode with specific response to glucose is obtained. during glucose detection, glucose can be specifically recognized and responded to in a neutral environment to rapidly detect the concentration of glucose, thereby having high sensitivity and strong anti-interference capability.
Description
technical field [0001] The invention belongs to the field of glucose detection, and in particular relates to a sensing electrode for glucose detection, a preparation method and an application. Background technique [0002] Glucose, as one of the main vital compounds, is very important in all fields of social production and life to establish a sensitive and specific detection method for its concentration. For example, in the medical field, with the improvement of living standards, the incidence of diabetes is increasing year by year. Reliable and sensitive blood glucose monitoring is particularly important for the early warning and treatment of diabetes. [0003] Various methods have been used for glucose detection, such as fluorescence spectroscopy, high performance liquid chromatography, gas chromatography, and colorimetry. However, these methods require complex equipment and high costs, making them often difficult to commercialize. Electrochemical glucose sensors are bei...
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IPC IPC(8): G01N27/30G01N27/327G01N27/48
CPCG01N27/308G01N27/3277G01N27/48
Inventor 李荣毓
Owner 李荣毓