Compound reagent for portable occult blood trace appearing as well as preparation method and using method of compound reagent
A portable and traceable technology, which is applied in the direction of analyzing materials through chemical reactions, material analysis and measuring devices by observing the influence of chemical indicators, etc. Complicated problems, to achieve the effect of short response time, good display effect and simple operation
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Embodiment 1
[0041] 1. Experimental method
[0042] 1.1 Reagents and instruments
[0043] Triaryl methane dyes, lactic acid, calcium chloride, citric acid, sodium sulfite, hydrogen peroxide, beaker, measuring cylinder, brown bottle, watering can.
[0044] 1.2 Sample making
[0045] Choose eight kinds of objects, such as glass slide, white tile, black tile, black leather, black tire, black paper, red brick, cement brick, etc., and use a dropper to drip human blood (100% concentration, diluted 10 times, diluted 100 times, Dilute 1000 times, dilute 10000 times) on the object, and then drip or spray on different objects. Each object was tested three times.
[0046] 1.3 Research on the method of displaying traces of occult blood
[0047] 1.3.1 The best formula of the compound reagent for the appearance of traces of occult blood
[0048] Solution A configuration: first dissolve 4g of calcium chloride in 400mL of distilled water, then add 4g of triarylmethane dye, 1mL of lactic acid, 125mg o...
Embodiment 2
[0076] The formula of the compound reagent for the appearance of traces of occult blood is:
[0077] Solution A configuration: first dissolve 4g of sodium chloride in 400mL of distilled water, then add 4g of triarylmethane dye, 1mL of lactic acid, 125mg of citric acid, 325mg of sodium sulfite, and stir to dissolve;
[0078] Configuration of solution B: first dissolve 0.4g sodium chloride in 45mL distilled water, add 5mL 30% hydrogen peroxide and transfer to a brown bottle.
[0079] Other operations are the same as in Example 1, and the obtained results are basically the same.
Embodiment 3
[0081] The formula of the compound reagent for the appearance of traces of occult blood is:
[0082] Solution A configuration: first dissolve 4g of calcium chloride in 400mL of distilled water, then add 4g of triarylmethane dye, 1mL of lactic acid, 125mg of citric acid, 325mg of sodium sulfite, and stir to dissolve;
[0083] Configuration of solution B: first dissolve 0.4g calcium chloride in 45mL distilled water, add 5mL 30% hydrogen peroxide and transfer to a brown bottle.
[0084] Solution A and solution B of the compound reagent are mixed at a volume ratio of 6:1 for visualization;
[0085] Other operations are the same as in Example 1, and the obtained results are basically the same.
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