Method for identifying and analyzing flavor components of colored sugar product
A technology of flavor components and analysis methods, which is applied in the field of food processing and safety testing, can solve the problems of inability to distinguish between brown sugar and brown sugar, not a pretreatment method, cover-up, etc., and achieve the effect of reducing incomplete collection
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Embodiment 1
[0064] Take 10g brown sugar sample and 25mL distilled water and mix evenly, take the colored sugar aqueous solution and place it in a 250mL Erlenmeyer flask with a stopper, add magnets and mix evenly for a certain period of time to prepare the colored sugar aqueous solution to be extracted. Take 50mL of n-pentane to extract the prepared colored sugar aqueous solution, stir with a magnet at 1000rmp for 5min, centrifuge at 12000rmp for 30min after 30min, take the upper organic phase to obtain the flavor component extract. Add 4g of anhydrous sodium sulfate to dry and remove water, filter, and place at -18°C for 4 hours. No ice debris appears, indicating that the water has been removed. Nitrogen is blown to 200 μL, and the injection volume is 1 μL to obtain a flavor component extraction concentrate. Using GC-O-MS equipment to perform gas chromatography-sniffing-mass spectrometry GC-O-MS analysis steps and aromatic extract dilution analysis steps, a total of 8 flavor components wit...
Embodiment 2
[0069] Take 10g brown sugar sample and 25mL distilled water and mix evenly, take the colored sugar aqueous solution and place it in a 250mL conical flask with a stopper, add magnets and mix evenly for a certain period of time to prepare the colored sugar aqueous solution to be extracted. Take 50mL of dichloromethane to extract the prepared colored sugar aqueous solution, stir with a magnet at 1000rmp for 5min, centrifuge at 12000rmp for 30min after 30min, take the upper organic phase to obtain the flavor component extract. Add 4g of anhydrous sodium sulfate to dry and remove water, filter, and place at -18°C for 4 hours. No ice debris appears, indicating that the water has been removed. Nitrogen is blown to 200 μL, and the injection volume is 1 μL to obtain a flavor component extraction concentrate. Using GC-O-MS equipment to perform gas chromatography-sniffing-mass spectrometry GC-O-MS analysis steps and aromatic extract dilution analysis steps, a total of 14 flavor components...
Embodiment 3
[0074] Take 10g brown sugar sample and 25mL distilled water and mix evenly, take the colored sugar aqueous solution and place it in a 250mL Erlenmeyer flask with a stopper, add magnets and mix evenly for a certain period of time to prepare the colored sugar aqueous solution to be extracted. Take 50mL of diethyl ether to extract the prepared colored sugar aqueous solution, stir with a magnet at 1000rmp for 5min, centrifuge at 12000rmp for 30min after 30min, take the upper organic phase to obtain the flavor component extract. Add 4g of anhydrous sodium sulfate to dry and remove water, filter, and place at -18°C for 4 hours. No ice debris appears, indicating that the water has been removed. Nitrogen is blown to 200 μL, and the injection volume is 1 μL to obtain a flavor component extraction concentrate. Using GC-O-MS equipment to perform gas chromatography-sniffing-mass spectrometry GC-O-MS analysis steps and aromatic extract dilution analysis steps, a total of 18 flavor component...
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