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Test kit for detecting various analytes

A technology of analytes and kits, which is applied in the field of inspection kits, can solve the problems of low load limit, weak signal, low concentration, etc., and achieve the effect of improving the preparation method and improving efficiency

Pending Publication Date: 2021-07-16
珠海宝龄创新生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, since the test strips in the immunochromatography kit have an upper limit on the maximum amount of carrying liquid, if it is desired to detect multiple analytes on the same test strip at the same time, when it is necessary to configure multiple specific antibodies, it will inevitably lead to Each specific antibody is lower than the concentration of a single antibody used to measure a single analyte
When there are more kinds of analytes, the upper limit of the concentration of various antibodies in the test strip will be lower, resulting in weaker signals and difficult to interpret

Method used

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  • Test kit for detecting various analytes
  • Test kit for detecting various analytes
  • Test kit for detecting various analytes

Examples

Experimental program
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preparation example Construction

[0109] In some embodiments, the preparation method of antibody-coupled microspheres comprises the following steps: providing microspheres, providing a washing solution; mixing the microspheres and the washing solution to obtain a first mixed solution; mixing the first mixed solution with 1-ethyl-3 -(3-dimethylaminopropyl) carbodiimide, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and the microspheres in the first mixed solution are activated reaction; mixing the activated microspheres and the coupling solution to obtain a second mixed solution; and mixing the first antibody and the second mixed solution, so that the first antibody and the microspheres in the second mixed solution are coupled at room temperature , to obtain antibody-coupled microspheres.

[0110] In one embodiment, the rinse solution includes 2-(N-morpholino)ethanesulfonic acid.

[0111] In one embodiment, the first mixed liquid is mixed with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, 1-ethyl-3-(3-dimet...

Embodiment 1

[0125] Embodiment 1, the preparation and composition of test set

[0126] First, in order to obtain a test kit that can simultaneously detect multiple analytes in the same sample, in this embodiment, four respiratory viruses (adenovirus, influenza A virus, influenza B virus, and respiratory virus Fusion virus) is used as the test substance, and is improved for the lysing solution in the test set and the solution in the test strip.

[0127] In the past lysing solutions, different formulas were required to lyse different viruses. Therefore, if four viruses need to be lysed, four lysing solutions with different components or component contents are required, which is not cheap for the manpower or time for preparation. cause a certain degree of loss. The lysing solution of this example (see Table 1) can effectively lyse the virus at the same time by using one formula, so that the four viruses can release the virus antigens. In this embodiment, the solvent of the lysing solution i...

Embodiment 2

[0143] Embodiment 2. Simultaneous detection of four kinds of respiratory viruses using the test kit

[0144] In order to further test the detection effect of the test set of Example 1, therefore, in the same respiratory tract sample, the detection limit of the test set for four kinds of respiratory viruses was determined, and compared with four kinds of commercially available sets (a set of individual Detect a respiratory virus) for comparison, the detection limit of the virus is expressed as the number of viruses that cause lesions in 50% of the cultured cells (Tissue Culture Infection Dose 50; TCID 50 ), and the results are shown in Table 4.

[0145] Table 4, the comparison of the detection limit of embodiment 1 test set and commercially available set

[0146]

[0147] The results show that the test kits provided by the embodiments of the present disclosure only need to test the specimen once, and can obtain the results that previously had to be tested separately by four...

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Abstract

The present disclosure provides a test kit for simultaneously detecting a plurality of analytes, in which the test kit includes a lysis solution and a test strip. The lysis solution includes a salt, a surfactant, a stabilizer, and a buffer solution. The test strip includes a sample pad, a conjugation pad, a cellulose membrane, and a water-absorbing pad sequentially arranged on a support plate. The conjugation pad includes a conjugation pad solution and a plurality of antibody-conjugated microspheres, and the antibody-conjugated microspheres recognize plurality of analytes. The test kit of the present disclosure achieves an effect of simultaneously detecting more than or equal to four analytes even though the conjugation pad has a limited capacity of the antibody by preparing the lysis solution with appropriate ingredients and improving a formulation of the solution contained in the test strip.

Description

technical field [0001] The present disclosure relates to test kits and methods thereof, and in particular to test kits and methods thereof capable of simultaneously detecting multiple analytes. Background technique [0002] Clinically, although many diseases originate from different pathogenic bacteria or viruses, they have similar clinical symptoms (such as respiratory diseases caused by respiratory viruses), resulting in a high risk of physician misjudgment. Although medical institutions have gradually introduced the use of testing kits (such as immunochromatography kits) for rapid screening of pathogens before treatment, in order to prescribe the right medicine and improve the treatment effect. However, since most of the current test kits are set in one sample, they can only be screened for a single analyte. If you want to test multiple analytes at the same time, it is often necessary to collect samples repeatedly, which will cause discomfort to the patient and affect th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/543
CPCG01N33/56983G01N33/54313
Inventor 施钧喨刘嘉惠
Owner 珠海宝龄创新生物科技有限公司