Protein biomarker for early pregnancy diagnosis of sows and method for early pregnancy diagnosis of sows

A technology of biomarkers and protein markers, which is applied in biological testing, biomaterial analysis, instruments, etc., can solve problems such as long diagnosis time, influence of user experience, and unclear diagnosis of symptoms, so as to shorten the parity interval , Reduce the number of non-production days, reduce the effect of production costs

Active Publication Date: 2021-07-16
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Traditional methods of pregnancy diagnosis, such as testing, external observation, ultrasonic diagnosis, vaginal examination, etc., have their own defects, such as long diagnosis time, unclear symptom diagnosis, and the influence of user experience, misdiagnosis, There are varying degrees of misdiagnosis

Method used

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  • Protein biomarker for early pregnancy diagnosis of sows and method for early pregnancy diagnosis of sows
  • Protein biomarker for early pregnancy diagnosis of sows and method for early pregnancy diagnosis of sows
  • Protein biomarker for early pregnancy diagnosis of sows and method for early pregnancy diagnosis of sows

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1: Screening of differential proteins in peripheral blood of pregnant and non-pregnant sows on the 15th day after mating by iTRAQ technology

[0048] In this example, the blood of pregnant and non-pregnant sows on the 15th day after mating was collected, the proteins were separated, and iTRAQ technology and bioinformatics methods were used to analyze and screen the differentially expressed proteins in the early pregnancy. The specific steps are as follows:

[0049] 1. Test sample

[0050] Eight normal-grown binary sows were randomly divided into control group and experimental group. According to the routine production process to check the estrus, the sows in the test group were injected with normal semen, and the sows in the control group were injected with dead semen (normal semen was boiled at high temperature and observed under a microscope to ensure that the sperm died). Blood was collected from pregnant sows and control sows on the 15th day after mating, a...

Embodiment 2

[0094] Example 2: Large group validation of differential protein expression in 15 days after breeding by ELISA between pregnant and non-pregnant peripheral blood

[0095] To further verify the protein expression results detected by iTRAQ, we used ELISA technology to verify the expression of the above four proteins in a large group.

[0096] ELISA kits for telomere repeat binding factor 2 (TERF2), haptoglobin (HP), adiponectin (ADIPOQ) and filamin C (FLNC) were purchased from Shanghai Hengyuan Biotechnology Co., Ltd.

[0097] 1. Sample information collection

[0098] On the 15th day after breeding, 21 sow blood samples were collected, and the plasma was separated, immediately placed in dry ice for cryopreservation, and transported back to the laboratory as soon as possible and stored in an ultra-low temperature refrigerator for future use.

[0099] 2. Sample information confirmation

[0100] After blood sample collection, B-ultrasound pregnancy test was performed on all breed...

Embodiment 3

[0142] Example 3: Application of protein biomarkers in the diagnosis of early pregnancy in sows

[0143] This example provides the application of a protein biomarker in the diagnosis of early pregnancy in sows. By detecting the expression level of protein biomarkers, we can judge whether the sow is pregnant. In this example, the ELISA enzyme-linked adsorption reaction was used as an example to diagnose early pregnancy. The ELISA kit was purchased from Shanghai Hengyuan Biotechnology Co., Ltd.

[0144] 1. Types and collection of blood samples

[0145] Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or overnight at 4°C, then centrifuge at 1000 × g for 20 minutes, take the supernatant, or store the supernatant at -20°C or -80°C , but repeated freezing and thawing should be avoided.

[0146] Plasma: collect specimens with EDTA or heparin as anticoagulant, centrifuge the specimens at 2-8°C at 1000×g for 15 minutes within ...

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Abstract

The invention discloses a protein biomarker for early pregnancy diagnosis of sows, which is characterized in that the protein biomarker is one of the following protein markers: FLNC protein with the sequence number of <210>1; ADIPOQ protein with the sequence number of <210>2; HP protein with the sequence number of <210>3; TERF2 protein with the sequence number of <210>4. The early pregnancy diagnosis method for the sows is characterized by comprising the following specific steps: (1) collecting blood samples: collecting peripheral blood of the sows 15 days after mating; (2) treating the blood samples: carrying out serum separation or plasma treatment on the blood samples in the step (1); and (3) performing detection with a kit: detecting the blood samples treated in the step (2) by using an ELISA kit.

Description

technical field [0001] The invention belongs to the field of biotechnology diagnosis, and particularly relates to a protein biomarker for early pregnancy diagnosis of sows and a method for diagnosing early pregnancy of sows. Background technique [0002] With the rapid development of my country's national economy and increasingly stringent environmental protection requirements, the way of raising pigs has rapidly changed from free-range to intensive. Breeding has become the most important part of the pig industry due to the increased demand for superior breeds. After sow breeding, timely and accurate pregnancy diagnosis is a key part of maximizing the reproductive efficiency of sows. Early pregnancy diagnosis of sows can confirm the pregnancy status of sows after mating. For pregnant sows, take measures to protect the fetus and corresponding pregnancy management in time. Repeatedly infertile sows should be culled in time. Early pregnancy diagnosis can reduce sow non-produ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/689G01N2333/47
Inventor 黄涛祁梦凡谢苏高若男孙义姗孙晓梅公红斌和军飞卢世豪李清春陈鑫王衠萱黎明国何凡
Owner SHIHEZI UNIVERSITY
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