Application of glucosamine as preharvest sprouting inhibitor in inhibition of preharvest sprouting of hybrid rice
A technology of glucosamine and hybrid rice, which is applied in the application field of glucosamine as an inhibitor of ear germination in inhibiting hybrid rice ear germination, can solve the problems of inconvenient use, achieve the goal of delaying germination rate, facilitating promotion, and reducing raw material costs Effect
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Embodiment 1
[0031] 1. Effects of different concentrations of glucosamine on rice seed germination
[0032] Use the chemical agent glucosamine with a concentration of 0, 100, 200, 300, 400, 500, 600, 700, 750, 800, 850, 1000mmol / L, and prepare them in aqueous solution, and add 0.02% (v / v) TWEEN20 at the same time .
[0033] Soak mature rice seeds (Nipponbare) in the above solution for 3-5 seconds, take them out, and dry them on tinfoil; then place them on moist filter paper, germinate at a constant temperature of 28°C, and count the germination rate every other day. The germination standard is that the germ or radicle grows 1mm and is germination.
[0034] The results show (Table 1), glucosamine above 100mmol / L can effectively delay the germination rate of seeds, compared with glucosamine lower than 700mmol / L, higher concentration of glucosamine (700-850mmol / L) delays the rate of seed germination. The effect of germination is more obvious, but when the concentration of glucosamine reache...
Embodiment 2
[0037]Example 2 Effects of Different Concentrations of Glucosamine on Ear Germination of Hybrid Rice
[0038] For the preparation of the panicle germination inhibitor, according to the results in Example 1, 3 concentrations of glucosamine aqueous solutions were prepared, namely 700, 750 and 850 mmol / L. In the indica rice hybrid Qian 6 You 688 seeding field, when the hybrid grows to the yellow maturity stage, the hybrid rice ear is collected, sprayed with the above-mentioned ear germination inhibitor to a saturated state, and dried in the shade on tin foil at room temperature for 24 hours, and then the whole rice ear is placed in a humid On the filter paper, spray water every 2-3 hours to a saturated state, germinate at a constant temperature of 28°C, and count the germination rate every 1 day. The germination standard is that the germ or radicle grows 1mm and is germination.
[0039] The results showed that the ear germination inhibitor glucosamine obviously delayed the ear g...
Embodiment 3
[0042] The impact of the copper sulfate aqueous solution of embodiment 3 different concentrations on rice seed germination
[0043] The present invention also studies the influence of using copper sulfate aqueous solution alone on the germination of rice seeds. The copper sulfate aqueous solution was used at a concentration of 0, 10, 20, 25, 30 and 35 mmol / L, and 0.02% (v / v) TWEEN20 was added at the same time. Soak mature rice seeds (Nipponbare) in the above solution for 3-5 seconds, take them out, and dry them in the shade on tinfoil. Then put it on wet filter paper, germinate at a constant temperature of 28°C, and count the germination rate every other day. The germination standard is that the germ or radicle grows 1mm and is germination. The result shows (table 2), and the copper sulfate solution above 25mmol / L has obvious inhibitory effect to rice seed germination, and the copper sulfate solution of 18-22mmol / L then obviously has the effect of delaying seed germination. ...
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