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A method of controlling the production of Bacillus cereus vomitoxin

A technology of Bacillus cereus and deoxynivalenol, which is applied in the field of controlling the production of Bacillus cereus vomitoxin, can solve the problems of increased synthesis of toxin, and achieve the effects of reduced production, low cost, and simple use

Active Publication Date: 2021-11-23
JINAN UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that external substances may have a regulatory effect at the gene level. The cesH in the ces gene cluster can inhibit the production of vomitoxin at the end of the logarithm.

Method used

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  • A method of controlling the production of Bacillus cereus vomitoxin
  • A method of controlling the production of Bacillus cereus vomitoxin
  • A method of controlling the production of Bacillus cereus vomitoxin

Examples

Experimental program
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Effect test

Embodiment 1

[0024] (1) Effect of lactose on the growth of emetic strains

[0025] First prepare a 30% lactose (w / v g / ml) solution (lactose is added to water), and filter and sterilize with a 0.22 μm water phase filter membrane. Then add 30% lactose solution into corresponding LB broth to prepare LB medium with lactose content of 0-5% (w / v g / ml) respectively. Five strains of pasteurized milk-derived nausea-causing strains (Bacillus cereus 892-1, Bacillus cereus 2132-4, Bacillus cereus 2833-2A, Bacillus cereus 3732, Bacillus cereus 3733-3C ) and emetogenic clinical reference strain Bacillus cereus F4810 / 72 (accession number: NCTC 11143) were inoculated into LB broth medium for recovery. Then dilute the bacterial solution to OD with LB broth 600 Approximately equal to 0.1. Pipette 100 μL of the bacterial solution into 1400 μL of LB medium with 5% lactose added (LA, that is, add lactose to the LB medium to make the content of 5 g / 100mL) or LB medium without lactose, mix well and then A vo...

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Abstract

The invention discloses a method for controlling the production of Bacillus cereus vomitoxin. Lactose is added to the substance that needs to control the vomitoxin produced by Bacillus cereus, and the vomitoxin produced by Bacillus cereus is controlled by lactose. The present invention starts with food ingredients and proposes that lactose is used as a food additive to significantly inhibit the synthesis of vomitoxin cereulide by bacillus cereus. It is of great significance to guide the addition of lactose and adjust the ratio of food ingredients to prevent and control Bacillus cereus-induced vomiting-type food poisoning. Lactose can be used as a new type of non-toxic and harmless food additive with low cost; the method of use is simple, and the direct addition of lactose can reduce the production of vomitoxin.

Description

Technical field: [0001] The invention belongs to the field of hazard control of food-borne pathogenic bacteria bacillus cereus, and in particular relates to a method for controlling the production of vomitoxin by bacillus cereus. Background technique: [0002] As a common conditional food-borne pathogen, Bacillus cereus widely exists in various environments, including water, soil, air, and all aspects of food storage and processing. Because Bacillus cereus is easy to form strong-tolerant spores, it can resist extreme conditions, such as extreme pH, high temperature, antibacterial agents, and environments such as ultraviolet rays or radiation. Therefore, it is easy to cause pollution during food storage and processing, and it is difficult to obtain raw materials or foods that do not contain Bacillus cereus spores. Bacillus cereus can contaminate different types of food. Foods that are generally susceptible to contamination include starchy foods, milk and milk products, meat ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L29/30A23C9/152A23L13/40
CPCA23C9/152A23V2002/00A23L13/42A23L29/30A23V2250/612
Inventor 丁郁郭卉吴清平王涓朱振军于鹏飞张俊卉陈诺徐文星张菊梅陈谋通吴诗曾海燕庞锐
Owner JINAN UNIVERSITY