Extraction and separation process of lactic acid bacteria fermentation extract

The technology of lactic acid bacteria fermentation and extract is applied in the field of extraction and separation technology of lactic acid bacteria fermentation extract, which can solve the problems of low extraction rate, low content of antioxidant factor and moisturizing factor, long production cycle, etc., and achieves improved extraction rate and extraction rate. Improve and promote the effect of fermentation

Pending Publication Date: 2021-07-23
绽妍生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Patent "A Bifidobacterium Lactic Acid Bacteria Mixed Fermentation Bacteria Enzymolysis Preparation Process and Its Application" Application No. 201511007402.5, Preparation of Expanded Strain Liquid and Primary Seed Culture Liquid The first-level strain liquid, and then the first-level strain liquid and the seed medium are mixed and cultivated in the seed tank to obtain the seed tank strain, and then the described seed tank strain is mixed with the fermenter culture medium and fermented by the induction liquid After the fermentation is completed, the fermentation liquid is centrifuged to obtain wet sludge. At present, most of the fermentation products of lactic acid bacteria are extracted by enzymatic hydrolysis or step by step, so the production cycle is long, the extraction rate is low, and it contains antioxidant Factors and low content of moisturizing factors

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  • Extraction and separation process of lactic acid bacteria fermentation extract
  • Extraction and separation process of lactic acid bacteria fermentation extract

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Embodiment 1

[0020] A process for extracting and separating lactic acid bacteria fermentation extracts: comprising the following steps:

[0021] S1. Fermentation: Lactobacillus is fermented and cultivated. The first fermentation temperature is 20°C and the fermentation time is 3h. The second fermentation temperature is 26°C and the fermentation time is 8h. The third fermentation temperature is 10°C and the fermentation time is 2h. , the number of colonies obtained is 1.0×10 8 The seed liquid of CFU / ml, described fermentation is carried out in shaker, controls the 160rpm of shaker, described fermentation medium formula: glucose 2g / L, sodium acetate 8g / L, peptone 10g / L, rose apple extract Prepared at 3 g / L; the rose apple extract: extract dried and crushed fresh leaves of rose apples with extractant for 10 hours, and then extract them at 20°C for 0.5 hours with 20KHz frequency ultrasonic waves to obtain rose apple extracts; The mass volume ratio of the fresh leaves of rose apple and the ext...

Embodiment 2

[0025] A process for extracting and separating lactic acid bacteria fermentation extracts: comprising the following steps:

[0026] S1. Fermentation: Lactobacillus is fermented and cultivated. The first fermentation temperature is 25°C and the fermentation time is 5h. The second fermentation temperature is 30°C and the fermentation time is 10h. The third fermentation temperature is 15°C and the fermentation time is 6h. , the number of colonies obtained was 3.0×10 8 The seed liquid of CFU / ml, described fermentation is all carried out in shaker, controls the 200rpm of shaker, described fermentation medium formula: glucose 8g / L, sodium acetate 10g / L, peptone 15g / L, rose apple extract 5g / L prepared; the rose apple extract: extract the dried and crushed fresh leaves of rose apple with extractant for 24h, and then extract it under the condition of 60°C for 1h under 50KHz frequency ultrasonic waves to obtain the roseberry extract; The mass volume ratio of the fresh leaves of rose ap...

Embodiment 3

[0030] A process for extracting and separating lactic acid bacteria fermentation extracts: comprising the following steps:

[0031] S1. Fermentation: Lactobacillus is fermented and cultivated. The first fermentation temperature is 23°C and the fermentation time is 4 hours. The second fermentation temperature is 28°C and the fermentation time is 9 hours. The third fermentation temperature is 13°C and the fermentation time is 4 hours. , the number of colonies obtained was 2.0×10 8 The seed liquid of CFU / ml, described fermentation is all carried out in shaker, controls the 180rpm of shaker, described fermentation medium formula: glucose 6g / L, sodium acetate 9g / L, peptone 13g / L, rose apple extract Prepared at 4 g / L; the rose apple extract: extract dried and crushed fresh leaves of rose apples with extractant for 17 hours, and then extract them at 40°C for 0.8 hours with 40KHz frequency ultrasonic waves to obtain rose apple extracts; Wherein, the mass volume ratio of the fresh lea...

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Abstract

The invention provides an extraction and separation process of a lactic acid bacteria fermentation extract. The process comprises the following steps of performing fermenting, extracting and separating to obtain the lactic acid bacteria fermentation extract, wherein the temperature and the time are adjusted in a three-stage temperature change mode to effectively improve the extraction rate of the lactic acid bacteria extract. The lactic acid bacteria fermentation extract has the certain anti-oxidation effect and moisturizing effect and can be applied to anti-oxidation daily chemical products.

Description

technical field [0001] The invention relates to the technical field of biological fermentation, in particular to an extraction and separation process of a lactic acid bacteria fermentation extract. Background technique [0002] Lactic acid bacteria (LAB) is a general term for bacteria that can use fermentable carbohydrates to produce large amounts of lactic acid. This kind of bacteria is widely distributed in nature and has rich species diversity, including at least 18 genera and more than 200 species in total. Except for a few, most of them are essential flora in the human body and have important physiological functions, and widely exist in the intestinal tract of the human body. Lactic acid bacteria are not only ideal materials for studying classification, biochemistry, genetics, molecular biology and genetic engineering, but also have extremely high application value in important fields closely related to human life such as industry, agriculture and animal husbandry, foo...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K8/99A61Q19/00A61Q19/08C12R1/23C12R1/225
CPCC12N1/20A61K8/99A61Q19/00A61Q19/08
Inventor 舒凌李嘉文
Owner 绽妍生物科技有限公司
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