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Platelet aggregation function arachidonic acid cup detection reagent and preparation method thereof

A technology of arachidonic acid and platelet aggregation, applied in the field of blood coagulation detection, can solve the problems of ineffective detection of patient samples, inability to truly reflect the degree of inhibition of platelets, lack of sensitivity, etc., and achieve good accuracy and good sensitivity , The effect of high batch-to-batch precision

Pending Publication Date: 2021-07-23
郑州普湾医疗技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] In view of the lack of sufficient sensitivity of thromboelastography (TEG) in detecting platelet aggregation function, it cannot effectively detect the samples of patients taking aspirin. Although some can be effectively detected, compared with other methods, they cannot truly reflect platelets The defects and problems of the degree of inhibition, the present invention provides a platelet aggregation function arachidonic acid detection reagent and its preparation method

Method used

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  • Platelet aggregation function arachidonic acid cup detection reagent and preparation method thereof
  • Platelet aggregation function arachidonic acid cup detection reagent and preparation method thereof
  • Platelet aggregation function arachidonic acid cup detection reagent and preparation method thereof

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Embodiment 1

[0024] Embodiment 1: The total amount of the arachidonic acid detection reagent in this embodiment is 40 microliters, and the consumption of each raw material is respectively 0.5% sodium arachidonic acid, 1.0% trehalose, 2.0% bovine serum albumin, 0.03% of dithiothreitol, 0.04% of dibutylhydroxytoluene, and the balance is purified water.

[0025] Preparation:

[0026] (1) Measure trehalose, bovine serum albumin, dithiothreitol and dibutylhydroxytoluene according to the formula, add pure water or purified water to fully dissolve to obtain a lyoprotectant solution;

[0027] (2) Add sodium arachidonic acid to the prepared freeze-drying protectant solution by weighing the formula amount, and mix uniformly to obtain the mother solution of sodium arachidonic acid detection reagent;

[0028] (3) Subpackage the sodium arachidonic acid detection reagent into the vial according to 40 microliters per bottle to obtain the finished product sodium arachidonic acid detection reagent;

[00...

Embodiment 2

[0030] Embodiment 2: The total amount of the arachidonic acid detection reagent in this embodiment is 60 microliters, and the dosages of each raw material by volume ratio are 1.0% sodium arachidonic acid, 2.0% trehalose, 3.0% bovine serum albumin, 0.06% of dithiothreitol, 0.06% of dibutylhydroxytoluene, and the balance is purified water.

[0031] Preparation:

[0032] (1) Measure trehalose, bovine serum albumin, dithiothreitol and dibutylhydroxytoluene according to the formula, add pure water or purified water to fully dissolve to obtain a lyoprotectant solution;

[0033] (2) Add sodium arachidonic acid to the prepared freeze-drying protectant solution by weighing the formula amount, and mix uniformly to obtain the mother solution of sodium arachidonic acid detection reagent;

[0034] (3) dispensing the sodium arachidonic acid detection reagent into the vials according to 60 microliters per bottle to obtain the finished product sodium arachidonic acid detection reagent;

[0...

Embodiment 3

[0036] Embodiment 3: The total amount of the arachidonic acid detection reagent in this embodiment is 40 microliters, and the consumption of each raw material is respectively 0.5% sodium arachidonic acid, 2.5% trehalose, and bovine serum albumin in terms of mass volume concentration. 2.0%, dithiothreitol 0.09%, dibutylhydroxytoluene 0.02%, and the balance is purified water.

[0037] Preparation:

[0038] (1) Measure trehalose, bovine serum albumin, dithiothreitol and dibutylhydroxytoluene according to the formula, add pure water or purified water to fully dissolve to obtain a lyoprotectant solution;

[0039] (2) Take sodium arachidonic acid according to the formula and add it to the prepared freeze-drying protective agent solution, and mix evenly to obtain the mother liquor of the arachidonic acid detection reagent;

[0040] (3) Subpackage the sodium arachidonic acid detection reagent into the vial according to 40 microliters per bottle to obtain the finished product arachido...

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Abstract

The invention belongs to the technical field of blood coagulation detection, and particularly relates to a platelet aggregation function arachidonic acid detection reagent and a preparation method thereof. The arachidonic acid detection reagent comprises sodium arachidonate and a freeze-drying protective additive, wherein the freeze-drying protective additive comprises a saccharide freeze-drying protective additive and a polymer freeze-drying protective additive; and different reactivity of the diagnostic reagent to antiplatelet drugs is fully considered, and the diagnostic reagent has good sensitivity to aspirin, ibuprofen and the like which are commonly used clinically at present. The arachidonic acid cup detection reagent is used for heparin sodium anticoagulant blood detection after medicine taking, the accuracy of the detection result is good, and the intra-batch and inter-batch precision is high.

Description

technical field [0001] The invention belongs to the technical field of blood coagulation detection, and in particular relates to a detection reagent for platelet aggregation function arachidonic acid and a preparation method thereof. Background technique [0002] Thromboelastography (TEG) is a coagulation function analyzer that implements real-time, dynamic and continuous monitoring of the entire process of platelet aggregation, coagulation, fibrinolysis, etc., and makes qualitative or quantitative analysis of the patient's coagulation status. [0003] Thromboelastography was invented by German Harter in 1948. It is used to guide blood transfusion during clinical operations. It has become one of the most important means to detect blood coagulation function. The principle of thromboelastography to detect blood coagulation function is to immerse the sensor probe in the blood sample. There is a viscous force between the blood sample and the sensor probe. The viscous force chang...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/88G01N33/86
CPCG01N33/88G01N33/86
Inventor 赵晓培张娟丽于婷李伟甲杨会孟兵涛
Owner 郑州普湾医疗技术有限公司