Cell image segmentation method based on anti-background differencing
A background subtraction and image segmentation technology, which is applied in the field of cell evaluation and cell image processing, can solve the problems that are not suitable for rapid and large-scale analysis of cell data, over-segmentation, and not suitable for cell segmentation, etc., to achieve high segmentation efficiency, eliminate halo, The effect of suppressing interfering factors
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Embodiment 1
[0029] figure 1 The flow chart of the cell image segmentation method of inverse background difference according to the embodiment of the present invention is given, such as figure 1 As shown, the cell image segmentation method of inverse background difference comprises the following steps:
[0030] (A1) noise reduction processing is performed on the acquired original cell image to obtain a first image;
[0031] (A2) performing inverse background difference processing on the first image to obtain a second image;
[0032] (A3) global threshold segmentation processing is performed on the second image to obtain a third image;
[0033] (A4) Performing hole filling and image filtering on the cell region in the third image to obtain a fourth image.
[0034] In order to improve the effect of image segmentation, further, the cell image segmentation method further includes the following steps:
[0035] (A5) Correcting the fourth image, the correction method is: performing morphologi...
Embodiment 2
[0058] An application example of the cell image segmentation method according to the inverse background difference of the embodiment 1 of the present invention in the evaluation of mesenchymal stem cells.
[0059] The cell image segmentation method of inverse background difference according to the embodiment of the present invention includes the following steps:
[0060] (A1) noise reduction processing is performed on the acquired original cell image to obtain a first image;
[0061] The specific method for obtaining the original image of the cell is as follows:
[0062] Cell recovery: add 9mL of complete cell culture medium to a 15mL sterile centrifuge tube, and place it in a 37°C water bath to preheat. Take out the cryovial containing adult bone marrow mesenchymal stem cells, put it in a 37°C water bath, and keep shaking until the contents are thawed. Use 70%-75% alcohol to disinfect the opening and outer wall of the cryopreservation tube and wipe it clean.
[0063] Open ...
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