A method for engineering synthetic cis-regulatory DNA

A purposeful and integrated technology, applied in the field of reporting carriers, can solve problems such as limitations
CN113166767APending Publication Date: 2021-07-23MAX DELBRUECK CENT FUER MOLEKULARE MEDIZIN
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
MAX DELBRUECK CENT FUER MOLEKULARE MEDIZIN
Publication Date
2021-07-23

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Abstract

The invention relates to methods for generating cell-type specific expression cassettes and reporter vectors, as well as nucleic acid constructs that can be generated by such methods. The cell-type specific expression cassettes and reporter vectors are characterized synthetic cis-regulatory DNA, also termed synthetic locus regions (sLCRs). sLCRs allow for a cell-type specific expression of reporter or effector genes. The invention further relates to various uses of the reporter vectors, including the determination of a property of a cell, preferably a cell type, state or fate transition, in gene and viral therapy, drug discovery or validation.
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Description

technical field

[0001] The present invention relates to methods for producing cell type-specific expression cassettes and reporter vectors, as well as nucleic acid constructs that can be produced by such methods. Cell type-specific expression cassettes and reporter vectors are characterized by synthetic cis-regulatory DNA, also known as synthetic locus regions (sLCRs). sLCRs allow cell type-specific expression of reporter or effector genes. The invention further relates to various uses of reporter vectors, including the determination of cell properties, preferably cell type, state or fate transition, in gene and viral therapy, drug discovery or validation. Background technique

[0002] Expression cassettes and reporter vectors have a wide range of applications in basic research, drug screening diagnostics, or gene therapy.

[0003] Selective identification of cell type-specific signatures is critical to understanding biological processes in which diverse cell types contrib...

Claims

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