Method for reducing leakage rate and improving transfer rate of tannin component of DASANGUO

A technology of leakage rate and transfer rate, which can be used in medical preparations containing active ingredients, plant/algae/fungus/moss ingredients, pharmaceutical formulations, etc. Quality extraction and purification, unsuitable for industrial production and other problems, to meet the needs of industrial production, improve the transfer rate and reduce production costs.

Pending Publication Date: 2021-08-06
张兰珍
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method only enriches the anticancer active ingredients of Myrobalan, and is not suitable for the extraction and purification of tannin
Moreover, the above two methods have not investigated the leakage rate of tannin components in the purification process, and are not suitable for industrial production.

Method used

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  • Method for reducing leakage rate and improving transfer rate of tannin component of DASANGUO
  • Method for reducing leakage rate and improving transfer rate of tannin component of DASANGUO
  • Method for reducing leakage rate and improving transfer rate of tannin component of DASANGUO

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0067] (a) Soak the HPD-400 type macroporous resin column in 95vol% ethanol aqueous solution for 4 hours, perform dynamic elution with 4BV of 95vol% ethanol aqueous solution, and then wash with water until the effluent has no alcohol smell;

[0068] (b) Soak in 5wt% hydrochloric acid aqueous solution for 3 hours, perform dynamic eluting with 3BV of 5wt% hydrochloric acid aqueous solution, and then wash with water until the effluent is close to neutral;

[0069] (c) Soak in 5wt% NaOH aqueous solution for 3 hours, perform dynamic elution with 3BV of 5wt% NaOH aqueous solution, and then wash with water until the effluent is neutral;

[0070] (d) Wash with 95vol% ethanol aqueous solution until the eluent and water are mixed at a volume ratio of 1:5 and there is no white turbidity, and then wash with water until the effluent has no alcohol smell, and an activated HPD-400 macroporous resin column is obtained.

Embodiment 1

[0072] 1) Take 30 g each of Amla emblica, Myrobalan myrobalan, and Myrobalan mulberry, mix, pulverize, and pass through a 10-mesh sieve to obtain a medicinal powder mixture. The drug powder mixture was placed in 70 vol% ethanol aqueous solution at 50° C. for 3 extractions, 6 hours each time. The volume ratio of the powder mixture to the aqueous ethanol solution is 1:10. Suction filtration, combined filtrates, to obtain Dasan fruit tannin extract. Put the tannin extract of Dasan fruit at 40°C, concentrate under reduced pressure to contain 0.2g of crude drug per ml of concentrated solution, and then centrifuge at 3000r / min for 30min to obtain a supernatant.

[0073] 2) Take 30ml of the supernatant and activate the HPD-400 macroporous resin column (the activation process is the same as that of Preparation Example 1, the diameter-to-height ratio is 1:7, and the wet volume of the resin is 20ml) for dynamic adsorption, and the dynamic adsorption speed is 3BV / h. After the adsorpt...

Embodiment 2

[0076] 1) Take 30 g each of Amla emblica, Myrobalan myrobalan, and Myrobalan mulberry, mix, pulverize, and pass through a 10-mesh sieve to obtain a medicinal powder mixture. The drug powder mixture was placed in 70 vol% ethanol aqueous solution at 50° C. for 3 extractions, 6 hours each time. The volume ratio of the powder mixture to the aqueous ethanol solution is 1:8. Suction filtration, combined filtrates, to obtain Dasan fruit tannin extract. Put the tannin extract of Dasan fruit at 40°C, concentrate under reduced pressure to contain 0.2g of crude drug per ml of concentrated solution, and then centrifuge at 3000r / min for 30min to obtain the supernatant.

[0077] 2) Take 30ml of the supernatant and activate the HPD-400 macroporous resin column (the activation process is the same as that of Preparation Example 1, the diameter-to-height ratio is 1:9, and the wet volume of the resin is 20ml) for dynamic adsorption, and the dynamic adsorption speed is 3BV / h. After the adsorp...

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Abstract

The invention discloses a method for reducing leakage rate and improving transfer rate of tannin component of DASANGUO, which comprises the following steps: performing dynamic adsorption, flushing and elution on supernate containing the tannin component of the DASANGUO by a macroporous resin column, and then drying the obtained eluent to obtain a tannin part of the DASANGUO; the ratio of the loading amount of the supernate to the volume of resin in the macroporous resin column is (2-4): 2, the diameter-height ratio of the macroporous resin column is 1: (7-9), and the dynamic adsorption speed is 2-4 BV / h. Wherein during macroporous resin enrichment and purification, the concentration of a sample loading solution, the sample loading amount of the sample loading solution, a washing solvent and the dosage of the washing solvent are key technologies of the method. The method can improve the leakproof effect of the tannin component of the DASANGUO and increase the transfer rate of the tannin component of the DASANGUO.

Description

technical field [0001] The invention relates to a method for reducing the leakage rate and improving the transfer rate of the tannin component of the three fruits. The total amount of leakage is controlled to determine the concentration of the macroporous resin sample solution and its sample volume, as well as the flushing solvent and its dosage, so as to improve the problem of low transfer rate of the tannins in the macroporous resin during the purification process. Background technique [0002] Dasanguo is a mixture of three flavors of Tibetan medicines: Terminalia chebula Retz, Terminalia bellirica (Gaertn.) Roxb) and Phyllanthus emblica Linn. Because the three flavors are often used in combination, or as The compatibility of their main ingredients is Sanguo Tang San, Sanguo Tang and other dosage forms, so they are called Dasanguo. Clinical application studies have shown that Dasanguo has the functions of scavenging free radicals, anti-oxidation, anti-inflammation, regul...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61K36/47A61P39/06A61P29/00A61P37/02A61P1/14A61P31/04A61P31/10A61P39/00A61P17/02A61P35/00A61P1/16A61P3/10A61P17/16A61K131/00
CPCA61K36/185A61K36/47A61P39/06A61P29/00A61P37/02A61P1/14A61P31/04A61P31/10A61P39/00A61P17/02A61P35/00A61P1/16A61P3/10A61P17/16A61K2236/55A61K2300/00
Inventor 张兰珍崔雅萍常子豪张秋楠刘宇琦
Owner 张兰珍
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