Gene detection kit for individualized medication of children asthma and application of gene detection kit

Abstract

The invention belongs to the field of gene detection, and particularly relates to a gene detection kit for individualized medication of children asthma and application of the gene detection kit. The kit mainly comprises a primer mixed solution, a multiple PCR library amplification buffer solution, a digestion buffer solution, a connection buffer solution, ligase, a specific joint, a HiFi library amplification buffer solution, a positive / negative quality control product, a report system and a description. The kit can be used for detecting common asthma-relieving medicines for children, and has 31 genes and 41 gene polymorphic sites; and related gene detection and report analysis for predicting the curative effect and the adverse reaction of the children asthma medicines are realized, and recommendation is provided for medication of clinicians.

Description

technical field [0001] The invention relates to the field of gene detection, in particular to a gene detection kit for detecting individualized medication for children's asthma and its application. Background technique [0002] Bronchial asthma (asthma for short) is a heterogeneous disease characterized by chronic airway inflammation. Airflow is restricted. [0003] Survey data show that the global average incidence of asthma in adults is 4.3%, while the incidence of asthma in children is 11.6% in children aged 6-7 and 13.7% in children aged 13-14. In my country, the survey data in 2010 showed that the overall prevalence of asthma among children aged 0-14 in urban areas was 3.02%, an increase of 52.8% compared with 2000, and the increase in large cities was even more significant. At the same time, the clinical control of asthma is not ideal. Data show that the uncontrolled rate of asthma in European adults is 45%; while the rate of uncontrolled asthma in Asian children is ...

AI Technical Summary

Problems solved by technology

[0007] In order to solve the problem that the above-mentioned existing testing products cover few drugs and genes, and the prediction is not accurate enough, new and effective treatment options are needed for patients; drugs involve many related genes and sites, and the interpretation is complicated, which is not conducive to clinicians' interpretation or Utilizing the detection results and other problems, the market needs an efficient and convenient kit for genetic testing of children's asthma drugs, which can improve the accuracy of children's asthma drugs and reduce the probability of adverse drug reactions. Based on this, the present invention provides a kit for Gene detection kit and its application for detecting individualized drug use in children with asthma

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