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Method for detecting 6-benzyladenine in biological fluid

A technology of benzyl adenine and detection method, which is applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problems of complicated pretreatment, low recovery rate and high false positive rate.

Pending Publication Date: 2021-08-06
HEBEI MEDICAL UNIVERSITY
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Problems solved by technology

[0005] At present, the methods for detecting plant auxins such as 6-benzyl adenine are mostly based on the research of fruit and vegetable matrices, and the instruments are mostly gas chromatography, liquid chromatography, gas chromatography-mass spectrometry, liquid chromatography-mass spectrometry, etc. These methods all have problems such as complex pretreatment, high false positive rate, and low recovery rate.
For the detection of 6-benzyl adenine in biological samples, and the application of electrostatic field orbitrap-high resolution mass spectrometry has not been reported yet

Method used

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  • Method for detecting 6-benzyladenine in biological fluid
  • Method for detecting 6-benzyladenine in biological fluid
  • Method for detecting 6-benzyladenine in biological fluid

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Embodiment 1

[0121] Accurately weigh 10 mg of 6-benzyl adenine, prepare 50 mL of a mixed solution with a concentration of 0.2 mg / mL with physiological saline, and store in a 4°C refrigerator for later use. Feeding healthy experimental Sprague-Dawley rats, aged 8-10 weeks, feeding environment: temperature 22°C-24°C, humidity 40%Rh-60%Rh, feeding regular feed and autoclaved water, adaptability Feed for 1 week, select 6 Sprague-Dawley rats with a body weight in the range of 250g-300g, fast for 12 hours before the experiment, one of them was used as a blank control, and the remaining 5 rats were given 6-benzyl gland by intragastric administration at a dose of 1mg / kg Purine solution, 6 rats were killed after 3 hours, blood and urine were collected, and samples were pretreated and analyzed as described in 1.3 and 1.4. The results are shown in the following table 4, the results show that the method can still detect 6-benzyl adenine at a lower concentration, and other literature reports (GC / MS det...

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Abstract

The invention discloses a method for detecting 6-benzyladenine in biological fluid. The method comprises the following steps: 1) pretreatment of the biological fluid, 2) liquid-liquid extraction, and 3) liquid phase-high resolution mass spectrum combined detection. The method is simple, convenient, rapid, cheap and efficient in pretreatment, high in recovery rate, good in reproducibility and good in specificity. Compared with the prior art, the method has the advantages that the detection limit advantage is obvious, in addition, the high-resolution mass spectrum first-level accurate mass number and second-level characteristic fragment ions are used for quantification and qualitation, and false positive and false negative misjudgment is greatly reduced. Therefore, the method not only can be used as a final confirmation method to be applied to detection of poisoning cases of 6-benzyladenine in biological fluid, but also can be used as an early monitoring method to be applied to health monitoring of related personnel.

Description

technical field [0001] The invention belongs to the field of medical detection, in particular to a method for detecting 6-benzyl adenine in biological fluids. Background technique [0002] 6-Benzyladenine (N-(phenylmethyl)-1H-Purin-6-amine) (6-BA) is a synthetic cytokinin whose main physiological function is to promote cell division and induce tissue differentiation. It has the following structure . It is often used in tissue culture and used in conjunction with auxin to increase fruit setting rate, promote fruit growth and vegetable preservation. In my country, 6-benzyl adenine, as one of the plant growth regulators registered by the Ministry of Agriculture, can be used in a limited range of crops such as fruits and vegetables. However, in recent years, 6-benzyl adenine abuse cases Occurs from time to time, and its potential harm to the human body has also become a hot spot of social concern. Therefore, it is very necessary to establish an accurate and rapid method for de...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/72
CPCG01N30/02G01N30/72G01N2030/027
Inventor 张晓光宋寒陈金婷魏萌贾凡张华兴付雪瑞聂文佳付炎
Owner HEBEI MEDICAL UNIVERSITY
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