Method for detecting 6-benzyladenine in biological fluid
A technology of benzyl adenine and detection method, which is applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problems of complicated pretreatment, low recovery rate and high false positive rate.
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[0121] Accurately weigh 10 mg of 6-benzyl adenine, prepare 50 mL of a mixed solution with a concentration of 0.2 mg / mL with physiological saline, and store in a 4°C refrigerator for later use. Feeding healthy experimental Sprague-Dawley rats, aged 8-10 weeks, feeding environment: temperature 22°C-24°C, humidity 40%Rh-60%Rh, feeding regular feed and autoclaved water, adaptability Feed for 1 week, select 6 Sprague-Dawley rats with a body weight in the range of 250g-300g, fast for 12 hours before the experiment, one of them was used as a blank control, and the remaining 5 rats were given 6-benzyl gland by intragastric administration at a dose of 1mg / kg Purine solution, 6 rats were killed after 3 hours, blood and urine were collected, and samples were pretreated and analyzed as described in 1.3 and 1.4. The results are shown in the following table 4, the results show that the method can still detect 6-benzyl adenine at a lower concentration, and other literature reports (GC / MS det...
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