Quantitative analysis method of acotamine in human plasma
A technology of acotiamide and quantitative analysis, applied in the field of biological analysis, can solve the problem of unreported quantitative detection, and achieve the effect of satisfying the recovery rate, strong specificity and high stability
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Embodiment 1
[0055] Embodiment 1: a kind of quantitative analysis method of acotiamide in human plasma
[0056] 1. Experimental materials and instruments
[0057] 1. Drugs and reagents: acotiamide hydrochloride (TLC); acotiamide hydrochloride-d6 (internal standard, TLC); acetonitrile (chromatographically pure, Merck); methanol (chromatographically pure, Merck); formic acid (chromatographic grade, Aladdin); Isopropanol (chromatographic grade, Yonghua Chemical Technology (Jiangsu) Co., Ltd.); Ammonium acetate (chromatographic grade, Aladdin); Ethyl acetate (chromatographically pure, Merck); tert-butyl methyl ether (chromatographically pure, Alfa); Ultrapure water (homemade, Millipore).
[0058] 2. Instruments: See Table 6 below for the names and specifications of specific instruments.
[0059] Table 6 Names and Specifications of Specific Instruments
[0060]
[0061]
[0062] 2. Liquid conditions
[0063] 1. Liquid chromatography conditions
[0064] Chromatographic column: Zorbax ...
Embodiment 2
[0140] Example 2: Quantitative analysis of acotiamide in human plasma using liquid-liquid extraction methods under different conditions
[0141] 1. Preparation of acotiamide working solution and internal standard working solution
[0142] Accurately weigh 1.007mg of acotiamide reference substance, after calculating the mass correction coefficient, add 0.878mL of methanol to prepare the acotiamide stock solution with a concentration of 1.00mg / mL, and use the acotiamide stock solution with Dilute the methanol-water solution with a volume ratio of 1:1 to a gradient of 0.4000ng / mL, 1.000ng / mL, 2.000ng / mL, 4.000ng / mL, 20.00ng / mL, 100.0ng / mL, 500.0ng / mL, 1000ng Acotiamide working solution at 1.200ng / mL, 1800ng / mL, 2000ng / mL; Repeat the above steps once to prepare acotiamide parallel working solution with gradient concentration of 1.200ng / mL, 60.00ng / mL, 1600ng / mL ; Preparation of internal standard (acotiamide-d6) standard solution: Accurately weigh 1.078 mg of acotiamide-d6 referen...
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