Method for detecting and screening exogenous risky substances in wine and application of method in detection

A detection method and wine technology, which can be applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of low detection efficiency, few analyzed varieties, and difficulty in meeting the needs of qualitative and quantitative rapid detection, so as to improve the screening efficiency and target species types. The effect of many, short analysis time

Pending Publication Date: 2021-08-06
KWEICHOW MOUTAI COMPANY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have good stability and detection limit, but these methods are limited to a single or several risk substances, and there are problems such as few types of analysis and low detection efficiency, and there is no effective rapid screening method, which is difficult to meet Qualitative and quantitative rapid detection needs of current food safety

Method used

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  • Method for detecting and screening exogenous risky substances in wine and application of method in detection
  • Method for detecting and screening exogenous risky substances in wine and application of method in detection
  • Method for detecting and screening exogenous risky substances in wine and application of method in detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Determination of exogenous risk substances in wine using chromatographic column (ThermoHypersil GOLD aQ)

[0039] experimental method:

[0040] 【Preparation of standard solution】

[0041] Accurately weigh 0.1000g of 27 standard substances into 100mL brown volumetric flasks, dissolve them with ultrapure water and make up to 100mL, prepare 1000mg / L standard stock solutions, and store them in a refrigerator at 4°C.

[0042] 【Sample pretreatment】

[0043] Take 100 μL wine sample into a 2 mL PV centrifuge tube, add 900 μL ultrapure water, mix well, centrifuge at 12000 rpm / min at 4 °C for 10 min, absorb the supernatant, and pass through a 0.22 μm microporous membrane.

[0044] 【Instrumental Analysis】

[0045] Perform ultra-high performance liquid chromatography-quadrupole electrostatic field orbitrap high-resolution mass spectrometry analysis and detection.

[0046] The ultra-high performance liquid chromatography conditions are: Thermo’s Hypersil GOLD aQ (150×2...

Embodiment 2

[0073] The influence of the selection of embodiment 2 different chromatographic columns on analytical effect

[0074] 27 target compounds from medium polarity to polarity, the present invention compares ZORBAX SB-C18 (2.1×100mm, 1.8μm), ACQUITY UPLC HSS T3 (2.1×100mm, 1.8μm), Hypersil GOLD aQ (150×2.1mm , 1.9μm) 3 kinds of chromatographic columns on the resolution, response value, peak shape, etc. of the target compound.

[0075] The chromatographic column is as follows, and the rest of the experimental steps are the same as in Example 1.

[0076] Column 1: ZORBAX SB-C18 (2.1×100mm, 1.8μm)

[0077] Column 2: ACQUITY UPLC HSS T3 (2.1×100mm, 1.8μm)

[0078] Column 3: Hypersil GOLD aQ (150×2.1mm, 1.9μm)

[0079] It was found that the concentration of natural sweeteners was higher than 100 μg / L, and the peaks on the ACQUITY UPLC HSS T3 chromatographic column bifurcated; when ZORBAX SB-C18 was used, the peak shape and retention were poor due to the high polarity of preservatives...

Embodiment 3

[0080] The impact of different dilution factors of embodiment 3 on the analysis effect

[0081] The influence of different dilution multiples on the analysis effect was analyzed, the dilution multiples are as follows, and the rest of the experimental steps are the same as in Example 1.

[0082] Dilution factor 1: Dilute 5 times with ultrapure water

[0083] Dilution factor 2: Dilute 10 times with ultrapure water

[0084] Dilution factor 3: dilute 20 times with ultrapure water

[0085] In this experiment, the influence of three different dilution factors on the analysis effect of the target was investigated, such as Figure 31 . If the dilution factor is too small, the content of interfering substances in the wine is high, and there is some interference to the target substance, resulting in a high or low recovery rate; if the dilution factor is too large, the target substance will be diluted, the peak shape will become poor, and the recovery rate will be unsatisfactory . W...

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Abstract

The invention provides a method for detecting compounds in wine. The method comprises ultra-high performance liquid chromatography-quadrupole electrostatic field orbitrap high-resolution mass spectrometry. The method for screening the exogenous risky substances in the wine, established by the invention, is sensitive and accurate, and can be suitable for quality control of the wine.

Description

technical field [0001] The invention relates to a substance detection method, in particular to a detection and screening method for exogenous risk substances in wine and its application in detection. Background technique [0002] Wine, as a kind of food, has paid more and more attention to its safety in recent years. The safety risks of wine exist in all aspects of production. The quality of raw materials, brewing water, fermentation process, filling, etc. may bring potential risks to wine security risks. The exogenous risk of wine refers to the harm caused by harmful substances introduced from the outside during the wine production process. These harmful risk substances mainly come from the brewing environment, raw materials used, production equipment, packaging materials, etc. It mainly includes food additives and illegal additives, pesticide residues, heavy metals, etc. Because additives are allowed to be used in the process of food production and processing, they shou...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/34G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/14G01N30/34G01N30/72G01N30/8631
Inventor 王莉聂叶曾稳稳刘松赵振宇王和玉
Owner KWEICHOW MOUTAI COMPANY
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