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Preparation method and application of carbofuran test paper based on SERS immunochromatography technology

An immunochromatography and carbofuran technology, which is applied in the field of test paper preparation, can solve the problem that the method for detecting carbofuran cannot be rapidly quantitatively and qualitatively detected, and achieves the effects of high recovery rate and good reproducibility.

Active Publication Date: 2021-08-10
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention provides a preparation method and application of carbofuran test paper based on SERS immunochromatography technology in order to solve the problem that the existing method for detecting carbofuran cannot be rapidly quantitatively and qualitatively detected

Method used

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  • Preparation method and application of carbofuran test paper based on SERS immunochromatography technology
  • Preparation method and application of carbofuran test paper based on SERS immunochromatography technology
  • Preparation method and application of carbofuran test paper based on SERS immunochromatography technology

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specific Embodiment approach 1

[0021] Specific embodiment one: the preparation method of the carbofuran test paper based on SERS immunochromatography technology in this embodiment is carried out according to the following steps:

[0022] Step 1, preparing carbofuran monoclonal antibody;

[0023] Step 2, preparing colloidal gold;

[0024] Step 3, labeling the Raman labeled molecule and the carbofuran monoclonal antibody prepared in step 1 on the colloidal gold to obtain the Raman immunoprobe;

[0025] Step 4, assembling the carbofuran test strip, the preparation of the test paper has been completed.

[0026] In step 3 of this embodiment, the Raman labeling molecule is 1mM PATP, and the obtained colloidal gold solution is bright red, uniform and transparent, and free of impurities visible to the naked eye. This colloidal gold is suitable for labeling monoclonal antibodies.

[0027] The preparation method of the Raman immune probe (SERS immune probe) in step 3 of this embodiment is:

[0028] 1. Take 1mL of ...

specific Embodiment approach 2

[0033] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the preparation method of the carbofuran monoclonal antibody in the step one: utilize furan phenol to prepare carbofuran artificial antigen, utilize immunized mouse to obtain hybridoma cell line, then Monoclonal hybridomas were prepared, separated and purified to obtain the monoclonal antibody of pandan. Other steps and parameters are the same as those in Embodiment 1.

[0034] The specific steps for preparing carbofuran monoclonal antibody in this embodiment are as follows:

[0035] 1. Preparation of carbofuran artificial antigen using furan phenol: Carbofuran hydrolyzed product furan phenol was used as raw material to prepare carbofuran artificial antigen

[0036] 1. Weigh 3-5g furanol, dissolve in 50mLCH 2 Cl 2 4.2 mL of pyridine was added under ice-bath conditions and stirred. 4.08g of p-nitrobenzoic chloroformate was dissolved in 35mLCH 2 Cl 2 In the above solution...

specific Embodiment approach 3

[0089] Specific embodiment three: the difference between this embodiment and specific embodiment two is: hybridoma cell line, which is hybridoma cell line 1D05, preserved in China Center for Type Culture Collection, preservation number is CCTCC NO: c2020243, preservation date for December 9, 2020. Other steps and parameters are the same as those in Embodiment 2.

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Abstract

The invention discloses a preparation method and application of carbofuran test paper based on an SERS immunochromatography technology, relates to a preparation method and application of test paper, and aims to solve the problem that the existing carbofuran detection method cannot realize rapid quantitative and qualitative detection. The preparation method of the test paper comprises the following steps: 1, preparing a carbofuran monoclonal antibody; 2, preparing colloidal gold; 3, preparing a Raman immune probe; and 4, assembling a carbofuran test paper strip. The test paper is applied to detection of carbofuran in food. According to the invention, the carbofuran monoclonal antibody and the Raman labeled immune probe are prepared, the immunochromatography test strip which has high characteristics on carbofuran and can be quantified is assembled, and a new method is provided for high-sensitivity rapid detection of harmful substances in food.

Description

technical field [0001] The invention relates to a preparation method and application of test paper. Background technique [0002] Pesticides and other chemical substances are needed in modern agriculture and food production, but the irrational or excessive use of these chemical substances has caused serious harm to humans and organisms, causing serious environmental and food safety problems. Excessive use of carbamate pesticides will pollute food and water in the environment, and human and animals will cause poisoning reactions after ingestion. Although carbamate pesticides are relatively short-lived in nature, they can cause severe neurotoxicity in humans. Among carbamate pesticides, carbofuran (2,3-dihydro-2,2-dimethyl-7-benzofuranmethyl carbamate) is a broad-spectrum insecticide commonly used in agriculture. Carbofuran is toxic to mammals by irreversibly inhibiting acetylcholinesterase activity through carbamylation of serine residues at synaptic junctions, resulting in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/532G01N33/577G01N21/65
CPCG01N33/558G01N33/532G01N33/577G01N21/65G01N33/184Y02A50/30
Inventor 孟利高思远武晋慧刘峰何静胡圣英
Owner HEILONGJIANG UNIV
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