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Kit for detecting DNA methylation degree of VGLL3 gene and application thereof

A detection kit and methylation technology are applied in the field of kits for detecting the degree of DNA methylation of VGLL3 gene, which can solve the problems of lack of disclosure, and achieve the effects of strong pertinence and high detection efficiency.

Active Publication Date: 2021-08-13
NINGBO FIRST HOSPITAL
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

At present, there is no public information about the VGLL3 Research report on the relationship between gene DNA methylation and cerebral aneurysm

Method used

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  • Kit for detecting DNA methylation degree of VGLL3 gene and application thereof
  • Kit for detecting DNA methylation degree of VGLL3 gene and application thereof
  • Kit for detecting DNA methylation degree of VGLL3 gene and application thereof

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Embodiment Construction

[0033] The present invention will be further described in detail below with reference to the embodiments of the accompanying drawings.

[0034] figure 1 is the invention VGLL3 Schematic diagram of the distribution position of the 12 CpG methylation sites detected in the region where the detected sequence of the gene is located; as shown in the figure, the specific location is hg38, Chr3: 86,990,862-86,990,913, the distribution position of the 12 CpG methylation sites detected in this region signal.

[0035] figure 2 is the invention VGLL3 Example diagram of gene methylation level detection results; the percentage shown in the figure is the methylation degree of the corresponding CpG site, as shown in the figure, the methylation degree of CpG1 to CpG12 is 16%, 11%, 15%, 14 %, 12%, 12%, 9%, 14%, 10%, 17%, 39%, 19% indicated.

[0036] image 3 is the difference between the cerebral aneurysm of the present invention and the control group VGLL3 Schematic diagram of gene DN...

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Abstract

The invention discloses a kit for detecting the DNA methylation degree of a VGLL3 gene and application thereof. The detection kit comprises a pair of specific amplification primers for DNA methylation of the VGLL3 gene and a specific sequencing primer for DNA methylation of the VGLL3 gene, wherein the specific amplification upstream primer has a nucleotide sequence as shown in SEQ ID NO.1, the specific amplification downstream primer has a nucleotide sequence as shown in SEQ ID NO.2, and the methylation specific sequencing primer has a nucleotide sequence as shown in SEQ ID NO.3; a region where a detected sequence of the VGLL3 gene DNA is located is a specific position at the front end of the gene, and DNA methylation level analysis is carried out through a pyrosequencing technology; the DNA methylation level of the VGLL3 gene is in negative correlation with the prevalence rate of cerebral aneurysm. The kit has the advantages that the kit can be used to detect the cerebral aneurysm on the molecular level conveniently and quickly, the detection efficiency is high, the pertinence is strong, and the medicine taking the DNA methylation of VGLL3 gene as the target spot is an effective means for auxiliary diagnosis, detection and screening of the cerebral aneurysm.

Description

technical field [0001] The invention relates to the technical field of auxiliary diagnosis of cerebral aneurysm, in particular to a detection VGLL3 Kit for DNA methylation degree of gene and its application. Background technique [0002] Cerebral aneurysm (IA) is a cystic tumor formed by the structural dysplasia of the arterial wall in the brain, or the damage or aging of the arterial wall caused by brain trauma and arteriosclerosis, which causes the local blood vessel wall to expand outward. Cerebral aneurysms are very easy to rupture suddenly when blood pressure increases due to occasional tension, exertion, fatigue, etc. The resulting cerebral subarachnoid hemorrhage and the serious complications caused by it are a great threat to human life and health, and the mortality rate and disability rates are high. For this reason, brain aneurysms are called "time bombs" of the brain. The etiology of cerebral aneurysms is still unclear, but congenital aneurysms account for the ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12Q1/6869
CPCC12Q1/6886C12Q1/6869C12Q2600/154C12Q2600/136C12Q2523/125C12Q2531/113C12Q2535/122C12Q2565/301
Inventor 黄毅
Owner NINGBO FIRST HOSPITAL
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