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Kit for detecting Seneca virus A antibody

A technology of Seneca and kit, which is applied in measurement devices, instruments, scientific instruments, etc., to achieve the effects of simple operation, good specificity and stable results

Pending Publication Date: 2021-08-13
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no commercial Seneca virus A antibody detection kit in China

Method used

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  • Kit for detecting Seneca virus A antibody
  • Kit for detecting Seneca virus A antibody
  • Kit for detecting Seneca virus A antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0014] The present invention is illustrated below in conjunction with examples.

[0015] 1. Preparation of kits

[0016] ① Seneca virus A propagation and inactivation: use IBRS-2 cells (porcine kidney cell line) to carry out the propagation of Seneca virus A, add 10% FBS and 1% double antibody (penicillin, streptavidin) in DMEM medium element), set 5% CO 2 Cultivate in an incubator at 37°C. When the IBRS-2 cell density reaches 80%, inoculate Seneca virus A (100 μL / bottle) (isolated from the horizontal skin of the diseased pig, preserved by our research team, accession number KY747510), about 12 hours later When the CPE reached 90%, the virus was harvested (stored at -30°C). When the amount of virus harvested is large (about 4000mL), add divinylimine (BEI) to inactivate (final concentration 1.5%, shake at 150rpm at 30°C for 28h), and then add blocking agent 50% sodium thiosulfate (sterilized , final concentration 2%) to block. Centrifuge at 4°C, 6500rpm for 30min, and disca...

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Abstract

The invention discloses a kit for detecting a porcine Seneca virus A antibody. The kit for detecting the Seneca virus A antibody at least comprises an elisa plate coated with Seneca virus A rabbit antiserum and Seneca virus A guinea pig antiserum. The kit has the advantages of high sensitivity, good specificity, simple operation and stable result, is suitable for batch detection, and can evaluate the infection condition of Seneca virus A in unimmunized swinery and the specific antibody level of swinery after vaccine immunization.

Description

technical field [0001] The invention relates to a virus antibody detection reagent, in particular to a kit for detecting porcine Seneca virus A antibody and a non-disease detection method for porcine Seneca virus A antibody. Background technique [0002] Senecavirus A (Senecavirus A, SVA) is the only member of the genus Senecavirus in the Picornaviridae family. The SVA genome is a single-stranded positive-strand RNA, non-segmented, infectious, and non-enveloped, with a total length of about 7.3 kb, containing 5' and 3' untranslated regions (UTRs), and a long open reading frame ( ORF). The 5'UTR contains an internal ribosome entry site (IRES) (similar in structure and function to that of classical swine fever virus, type IV), with a poly(a) tail at the 3', and the polyprotein encoded by the ORF consists of a leader (L ) and three major protein domains (P1, P2 and P3), which are cleaved to produce 12 mature proteins (5'-L-VP4-VP2-VP3-VP1-2A-2B-2C-3A-3B-3C-3D- 3'). It is cl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569
CPCG01N33/56983
Inventor 冯霞马军武郭慧琛孙世琪张韵白满元郭丽莹
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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