Application of purine metabolism marker in preparation of reagent for screening and diagnosing acquired drug resistance of lung cancer molecular targeted drug

A molecular targeting drug and metabolic marker technology, applied in material separation, analysis materials, instruments, etc., can solve the problems of poor sensitivity, low detection efficiency, limited clinical value, etc., and achieve good guiding significance.

Active Publication Date: 2021-08-17
SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, after the initial treatment is effective, the tumor will inevitably acquire drug resistance to EGFR-TKI, which greatly limits the ultimate clinical value of this type of drug
[0003] At present, the most widely used clinical drug resistance detection

Method used

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  • Application of purine metabolism marker in preparation of reagent for screening and diagnosing acquired drug resistance of lung cancer molecular targeted drug
  • Application of purine metabolism marker in preparation of reagent for screening and diagnosing acquired drug resistance of lung cancer molecular targeted drug
  • Application of purine metabolism marker in preparation of reagent for screening and diagnosing acquired drug resistance of lung cancer molecular targeted drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0035] Example. Detection of Purine Metabolites Based on High Performance Liquid Chromatography-High Resolution Mass Spectrometry

[0036] In order to detect the levels of purine metabolites in the biological samples of the molecularly targeted drug-acquired drug-resistant group and the sensitive group, the response intensity of the metabolites in the samples was obtained through pretreatment operations such as metabolite extraction and protein removal.

[0037] Materials and Reagents

[0038] 1) Instruments: ultra-high performance liquid chromatography (Nexera UPLC System, Shimadzu, Japan), quadrupole time-of-flight mass spectrometry (TripleTOF TM 5600plus, SCIEX, USA), etc.;

[0039] 2) Reagent consumables: chromatographic grade methanol and acetonitrile, LCMS grade formic acid, ammonia water and ammonium bicarbonate were purchased from MERCK Chemical Company in Germany; ultrapure water was obtained from Integral 3 pure water system; ZIC-HILIC column (150mm x 2.1mmi.d., ...

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Abstract

The invention provides application of a purine metabolism marker in preparation of a reagent for screening and diagnosing acquired drug resistance of a lung cancer molecular targeted drug. The purine metabolism marker is one or more of adenosine monophosphate, guanosine monophosphate, inosine monophosphate, xanthosine monophosphate, adenosine, guanosine, inosine, xanthosine, adenine, guanine, hypoxanthine, xanthine and uric acid. The metabolic marker has good diagnostic potential for acquired drug resistance of the lung cancer molecular targeted drug, can be used for diagnosis and curative effect monitoring of acquired drug resistance of the lung cancer molecular targeted drug, and has good guiding significance for development of subsequent clinical application research.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to the application of purine metabolic markers in the preparation of acquired drug resistance screening and diagnostic reagents for lung cancer molecular targeting drugs. Background technique [0002] Lung cancer is the malignant tumor with the highest mortality rate in my country. For lung cancer with activating mutations of epidermal growth factor (EGFR), molecular targeted drug therapy such as epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKI) targeting the driver gene has made significant progress. The proportion of lung cancer patients with EGFR activating mutations in the East Asian population is much higher than that of European and American patients. Therefore, EGFR-TKI targeted therapy is of great significance to Chinese lung cancer patients. However, after the initial treatment is effective, the tumor will inevitably acquire drug resistance to EGFR-...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 朱亮唐亚斌陈红专雷绘敏张可人吕倩明沈瑛张雨霏马春爽徐光旎
Owner SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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