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Molecular marker-assisted breeding method of gibberellic disease-resistant high-yield wheat

A molecular marker-assisted, scab-blight technology, applied in the fields of botanical equipment and methods, biochemical equipment and methods, and microbial assay/inspection to achieve the effect of improving breeding efficiency

Active Publication Date: 2021-08-20
JIANGSU LIXIAHE REGION AGRI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there has been no report of using traditional resistance sources or resistance genes to breed breakthrough varieties that can be widely promoted in production and have scab resistance comparable to that of Sumai 3 (R grade).

Method used

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  • Molecular marker-assisted breeding method of gibberellic disease-resistant high-yield wheat
  • Molecular marker-assisted breeding method of gibberellic disease-resistant high-yield wheat
  • Molecular marker-assisted breeding method of gibberellic disease-resistant high-yield wheat

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 A molecular marker-assisted breeding method for scab-resistant high-yield wheat

[0035] Parent selection: use the CTAB method to extract DNA from mixed leaves of wheat varieties with a cumulative extension area of ​​more than 5 million mu, good comprehensive disease resistance and comprehensive agronomic traits, or their derivative varieties (lines) at the seedling stage, and molecular markers to screen out the number of grains carried at the same time The female target variety Zhenmai 9 of the synergistic site QGns-3B and the grain weight synergistic site QGW-Y4; and the male target variety Yangmai 18 carrying the main genes Fhb1 and Fhb5 for resistance to head blight were screened out.

[0036] In the screening results, it was found that "wheat varieties with a cumulative promotion area of ​​more than 5 million mu, excellent agronomic traits, and good comprehensive disease resistance or their derivative varieties (lines)" Zhenmai 9, Zhenmai 12, Yangmai 16, a...

Embodiment 2

[0045] Example 2 Establishment of molecular marker-assisted selection of grain number per spike locus QGns-3B and scab resistance gene Fhb5

[0046] The primer sets of molecular markers Xgpw1145, stm7tgag, WMC752, and MAG9482 were used to detect whether the materials in steps 1), 4) and 5) in Example 1 carried the grain number per spike site QGns-3B and the head blight resistance gene Fhb5.

[0047] 1. With the genomic DNA extracted in step 1) as a template, use the QGns-3B site linkage marker Xgpw1145 and stm7tgag for detecting wheat ear grain number synergistic sites to carry out PCR amplification to obtain the amplified product, and determine the QGns-3B site The target material Zhenmai No. 9 with the same genotype as Yangmai 17 was selected; the linkage markers WMC752 and MAG9482 used to detect wheat head blight resistance gene Fhb5 were used for PCR amplification to obtain the amplified product, and the Fhb5 locus was determined to be related to Wangshuibai The target mat...

Embodiment 3

[0055] Example 3 Establishment of molecular marker-assisted selection method for scab resistance gene Fhb1

[0056] The primer set of molecular marker TaHRC-GSM was used to detect whether the materials in steps 1), 4) and 5) in Example 1 carried the head blight resistance site Fhb1.

[0057] 1, with the genomic DNA extracted in step 1) as a template, the linkage marker TaHRC-GSM that is used to detect the scab-resistant gene of wheat described in Example 1 is used to carry out PCR amplification, obtain the amplified product, and determine the Fhb1 site and The genotype of Sumai 3 is the same as the target material Yangmai 18.

[0058] 2. Extract Zhenmai No. 9, Yangmai 18 and F by CTAB method 3 single plant, F 4 Genomic DNA from the leaves was mixed in the plant row and diluted to obtain a template solution with a DNA concentration of about 30 ng / μL.

[0059] The sequence of the specific primer set of the linked GSM marker for the detection of scab resistance gene Fhb1 by mo...

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Abstract

The invention discloses a molecular marker-assisted breeding method of gibberellic disease-resistant high-yield wheat. The molecular marker-assisted breeding method of the gibberellic disease-resistant high-yield wheat is characterized in that a grain number synergy site QGns-3B, a grain weight synergy site QGW-Y4 and gibberellic disease-resistant major genes Fhb1 and Fhb5 are polymerized on the background basis of a wheat variety or a derived variety (line) thereof with excellent agronomic characters and good comprehensive disease resistance; for low generations, comprehensive agronomic characters such as plant height, lodging resistance and growth period are selected, F3 begins to be screened in combination with molecular markers, F4 is selected to be planted into plant rows in a field, the comprehensive agronomic characters, gibberellic disease resistance, comprehensive disease resistance and the like are selected, yield-related character synergistic sites and gibberellic disease-resistant genes are polymerized in an auxiliary mode through the molecular markers, ; after harvesting, the thousand seed weight and yield are measured, F5 and then enter an identification nursery and a variety comparison nursery, comprehensive disease resistance, the comprehensive agronomic characteristics and yield identification are comprehensively carried out, and a gibberellic disease-resistant high-yield wheat variety is cultivated.

Description

technical field [0001] The invention belongs to the technical field of wheat molecular breeding methods, and relates to a molecular marker-assisted breeding method for scab-resistant and high-yield wheat. Background technique [0002] Yangmai No. 4 is a wheat variety bred by the Agricultural Science Research Institute of Lixiahe District, Jiangsu Province. It has excellent traits such as early maturity, high yield, and disease resistance. Among the three elements of yield, thousand-grain weight is particularly outstanding. 45.5g to 47.5g, significantly higher than common varieties by 5.56% to 13.10%. Yangmai 5, Yangmai 158 and other wheat varieties with breakthroughs in yield and disease resistance were bred with Yangmai 4 as the parent. Derived varieties Yangmai 11 and Yangmai 16, etc., the first two varieties won the first prize of the National Science and Technology Progress Award, becoming the wheat variety with the largest planting area in the late 1980s and the end of th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04C12Q1/6895C12N15/11
CPCA01H1/02A01H1/04C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 胡文静张勇高德荣吕国锋张晓祥朱冬梅蒋正宁张春梅江伟吴素兰
Owner JIANGSU LIXIAHE REGION AGRI RES INST