Application of lauromacrogol in preparation of chronic intraocular hypertension animal model and animal model

An animal model, the technology of lauryl alcohol, applied in animal husbandry and other directions, can solve the problems of retinal damage, difficulty in accurately simulating high intraocular pressure on the retina, etc., and achieve the effect of promoting thrombosis.

Inactive Publication Date: 2021-08-20
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of these drugs are injected intraocularly, and the injection itself may cause direct damage to the retina. It is difficult to accurately simulate the impact of high intraocular pressure on the retina, and there are certain drawbacks.
In addition, except for transgenic animals, there is currently no drug injection method that can establish a stable chronic ocular hypertension model

Method used

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  • Application of lauromacrogol in preparation of chronic intraocular hypertension animal model and animal model
  • Application of lauromacrogol in preparation of chronic intraocular hypertension animal model and animal model
  • Application of lauromacrogol in preparation of chronic intraocular hypertension animal model and animal model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] This example is used to establish an animal model of chronic ocular hypertension.

[0045] Preparation of model animals:

[0046] Select 40 healthy SD male rats aged 7-8 weeks, and randomly divide them into two groups, 20 in each group, as model group and control group, respectively. Raise in the environment of temperature 20-26 ℃, relative humidity 60%, free access to food and water for 1 week. 3 days before the operation, measure the intraocular pressure when awake at 8-10 o'clock every day, and record the average intraocular pressure 1 day before the operation as the baseline intraocular pressure.

[0047] Establishment of animal models:

[0048] The establishment of the animal model is as follows figure 1 As shown, it mainly includes the following steps:

[0049]S10, model animal is anesthetized: after the abdominal cavity of the rat of model group and matched group is injected 2% sodium pentobarbital by the dose of 80mg / kg (0.3ml / 100g) and carries out general a...

Embodiment 2

[0059] This embodiment is used to detect the high intraocular pressure of model animals and the changes in intraocular tissue caused by it.

[0060] One day, one week, two weeks, three weeks, four weeks, two months, and six months after lauromacrogol injection in the model group rats, the intraocular pressure of the rats in the awake state was measured with a tonometer, and the performance of the front of the eye was observed and analyzed. Changes in intraocular pressure at different time points, measurement results such as Figure 4 shown. Depend on Figure 4 It can be seen that the intraocular pressure of the rat measured 5 minutes after the injection of lauromacrogol was 17.5 ± 1.0 mmHg. One week after lauromacrogol injection, the intraocular pressure increased to 23.2±3.5mmHg, and the eyes of the rats showed hyperemia of the bulbar conjunctiva, no corneal edema, no significant change in pupil diameter, and no obvious turbidity in the anterior chamber. From the 3rd week ...

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Abstract

The invention discloses application of lauromacrogol in preparation of a chronic intraocular hypertension animal model. The chronic intraocular hypertension animal model can be constructed by injecting lauromacrogol into suprascleral veins of a model animal, especially an animal model with chronic mild intraocular pressure rise. The model construction method is simple, the intraocular hypertension maintaining time is long, and complications do not exist. The chronic intraocular hypertension animal model can be used for simulating the chronic intraocular pressure rising process of open-angle glaucoma of people, is suitable for animal experiment research of screening glaucoma treatment drugs and glaucoma pathogenesis research, and has the application value of preclinical research.

Description

technical field [0001] The present invention relates to animal disease models, in particular to the application of lauromacrogol in the preparation of chronic high intraocular hypertension animal models. The invention also relates to an animal model of chronic high intraocular pressure and the application of the animal model of chronic high intraocular pressure in the experimental research of glaucoma disease. Background technique [0002] Glaucoma is a type of irreversible blinding eye disease mainly manifested by optic nerve damage and visual field defect. The essence is the irreversible death of retinal ganglion cells (RGCs). The main risk factor is pathological intraocular pressure ( Intraocular Pressure, IOP) increased. Its morbidity rate is high, prognosis is poor, and it is a difficult point in the treatment of clinical eye diseases. [0003] At present, common glaucoma chronic ocular hypertension animal models and their characteristics are: (1) the ocular hypertens...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027
CPCA01K67/027A01K2207/20A01K2267/03A01K2227/105
Inventor 夏晓波夏明芳张恩东李海波姚飞
Owner CENT SOUTH UNIV
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