Metabonomics analysis method based on cardiac shock rat model

Abstract

The invention relates to a metabonomics analysis method based on a cardiac shock rat model. The method comprises the following steps of: copying early and middle cardiac shock rat models by adopting a left anterior descending coronary branch proximal tip and telecentric tip ligation method; acquiring metabolic map information of early-stage and middle-stage plasma samples of cardiac shock of rats on the basis of an ultra-high performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometer; and performing data preprocessing by using Progenense QI, performing multivariate statistical analysis by using EZinfo 2.0 statistical software, screening out a biomarker group with significant difference, analyzing a metabolic pathway in which the biomarker group participates through a Metaboanist.ca website, and discussing an action mechanism of treating early and middle stages of cardiac shock by using the ginseng-radix aconiti carmichaeli injection. According to the invention, the influence and change rule of external stimulation on the endogenous micromolecules of the living body are researched from the overall perspective, and a new research thought and method are provided for the research on the syndromes of traditional Chinese medicine and the overall action mechanism of the traditional Chinese medicine.

Description

technical field [0001] The invention belongs to the field of bioinformatics and relates to a metabolomics analysis method based on a cardiogenic shock rat model. Background technique [0002] Cardiogenic shock is due to insufficiency of the heart, leading to pump failure, which makes the surrounding tissues in a state of hypoperfusion and abnormal hemodynamics, thereby inducing shock. According to the occurrence and development of cardiogenic shock, it can be divided into early, middle and late stages. In the early stage, clinical hemodynamic indicators are as follows: blood pressure is normal or even slightly increased or slightly decreased, heart rate is accelerated, and the pulse pressure difference is reduced; The hemodynamic parameters are as follows: the blood pressure drops significantly, the heart rate increases faster than in the early stage, and the pulse pressure difference further decreases compared with the early stage; in the late stage, symptoms of disseminate...

AI Technical Summary

Problems solved by technology

At present, there are few studies on the mechanism of action of Shenfu injection in the treatment of cardiogenic shock, and there is no clear classification of cardiogenic shock stages