Joint detection kit for two pathogenic bacteria of gonococcus and chlamydia trachomatis

A technology for combined detection of Chlamydia trachomatis, applied in biological testing, measuring devices, immunoassays, etc., can solve the problems of many kit components, NaOH false positives, complicated operations, etc. The result, the effect of simple operation

Pending Publication Date: 2021-08-20
山东康华生物医疗科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The kit has many components and complex operation, and it is necessary to mix the lysate A and lysate B in sequence and in the same volume ratio, otherwise it is easy to cause false negatives caused by too much NaOH or false positives caused by too much HCl; detection Neisseria gonorrhoeae and Chlamydia trachomatis require different components of lysate A and lysate B. The components of the lysate for the detection of gonorrhoeae and the lysate for the detection of Chlamydia trachomatis are different, requiring multiple containers and many accessories. Two Sampling once, cracking twice, complex operation

Method used

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  • Joint detection kit for two pathogenic bacteria of gonococcus and chlamydia trachomatis

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Effect test

Embodiment 1

[0029] Embodiment 1: a kind of Neisseria gonorrhoeae, Chlamydia trachomatis two kinds of pathogenic bacteria joint detection kits, comprise detection card and lysate, described detection card comprises gonorrhea detection test strip and Chlamydia trachomatis detection test strip, described Neisseria gonorrhoeae detection test strip Both the test strip and the Chlamydia trachomatis test strip include absorbent paper, a bottom plate, a sample pad, a solid-phase antibody reaction membrane and a gold-labeled antibody reaction membrane. The solid-phase antibody reaction membrane is pasted in the middle of the bottom plate, and the gold-labeled antibody reaction membrane is on Below the solid-phase antibody reaction membrane, the sample pad is located under the gold-labeled antibody reaction membrane, the absorbent paper is placed above the solid-phase antibody reaction membrane, and the bottom plate is a PVC board. After flattening the product obtained in the above steps, cut it into...

Embodiment 2

[0058] Embodiment 2: a kind of Neisseria gonorrhoeae, Chlamydia trachomatis two kinds of pathogenic bacteria joint detection kits, comprise detection card and lysate, described detection card comprises gonorrhea detection test strip and Chlamydia trachomatis detection test strip, described Neisseria gonorrhoeae detection test strip Both the test strip and the Chlamydia trachomatis test strip include absorbent paper, a bottom plate, a sample pad, a solid-phase antibody reaction membrane and a gold-labeled antibody reaction membrane. The solid-phase antibody reaction membrane is pasted in the middle of the bottom plate, and the gold-labeled antibody reaction membrane is on Below the solid-phase antibody reaction membrane, the sample pad is located under the gold-labeled antibody reaction membrane, the absorbent paper is placed above the solid-phase antibody reaction membrane, and the bottom plate is a PVC board. After flattening the product obtained in the above steps, cut it into...

Embodiment 3

[0087] Embodiment 3: a kind of gonorrhea, Chlamydia trachomatis two kinds of pathogenic bacteria joint detection kits, comprise detection card and lysate, described detection card comprises gonorrhea detection test paper strip and Chlamydia trachomatis detection test paper strip, described Neisseria gonorrhoeae detection test strip Both the test strip and the Chlamydia trachomatis test strip include absorbent paper, a bottom plate, a sample pad, a solid-phase antibody reaction membrane and a gold-labeled antibody reaction membrane. The solid-phase antibody reaction membrane is pasted in the middle of the bottom plate, and the gold-labeled antibody reaction membrane is on Below the solid-phase antibody reaction membrane, the sample pad is located under the gold-labeled antibody reaction membrane, the absorbent paper is placed above the solid-phase antibody reaction membrane, and the bottom plate is a PVC board. After flattening the product obtained in the above steps, cut it into...

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Abstract

The invention relates to a joint detection kit for two pathogenic bacteria of gonococcus and chlamydia trachomatis, wherein the joint detection kit comprises a detection card and a lysate; the detection card comprises a gonococcus detection test strip and a chlamydia trachomatis detection test strip; the gonococcus detection test strip and the chlamydia trachomatis detection test strip both comprise sample pads; and the lysate comprises 0.05-0.15 M of a NaOH solution, 0.15 M of a Na2HPO4 solution, 0.005-0.01% by mass of NP40 and 0.005-0.01% by mass of X-100. The lysate adopted in the kit is alkaline, the sample pads of the two pathogenic bacteria are acidic, one-time sampling and one-time sample treatment are achieved by changing the components of treating fluids of the two sample pads, detection of the two pathogenic bacteria is completed at the same time, and operation is convenient and rapid.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnosis, in particular to a combined detection kit for two pathogenic bacteria, Neisseria gonorrhoeae and Chlamydia trachomatis. Background technique [0002] Neisseria gonorrhoeae, also known as Neisseria gonorrhoeae, is a strict human parasite, mainly manifested as purulent infection of the genitourinary tract. Urethritis and cervicitis are more common. If not treated in time, Neisseria gonorrhoeae can invade the genitourinary tract. In other parts, it can cause inflammation in the epididymis, prostate, fallopian tubes, pelvic cavity and other organs, and can even spread to other parts of the body through the blood, forming gonorrhea arthritis, tenosynovitis, endocarditis, meningitis and other complications. [0003] Chlamydia trachomatis is a strictly intracellular parasitic prokaryotic microorganism that can invade the eyes, reproductive tract and other organs. Urogenital tract infection c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558G01N33/577G01N33/58G01N33/531G01N33/532
CPCG01N33/531G01N33/532G01N33/558G01N33/56911G01N33/56927G01N33/577G01N33/587G01N2333/22G01N2469/10
Inventor 杨帆曲文英翟玉娥杨明霞曹传美高晓晓
Owner 山东康华生物医疗科技股份有限公司
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