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Isolation medium for detecting Escherichia coli O157 and preparation method thereof

A technology for separating medium and content, applied in the field of microbial detection, can solve problems such as interference with Escherichia coli, inconsistent fermentation rate of sorbitol, etc., and achieve the effect of optimizing content and reducing false positive rate

Pending Publication Date: 2021-08-24
SHANGHAI INST FOR FOOD & DRUG CONTROL
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, in the actual detection, some microorganisms such as Enterobacteriaceae and Proteus do not ferment sorbitol and appear as colorless colonies; in addition, the fermentation rate of microorganisms to sorbitol is inconsistent, which leads to the colorless colonies when the red color of the colonies fades within the observation time range. Factors interfere with the detection of Escherichia coli O157:H7 / HM

Method used

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  • Isolation medium for detecting Escherichia coli O157 and preparation method thereof
  • Isolation medium for detecting Escherichia coli O157 and preparation method thereof
  • Isolation medium for detecting Escherichia coli O157 and preparation method thereof

Examples

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Embodiment 1

[0034] This embodiment provides a kind of separation culture medium that detects Escherichia coli O157, every 1000mL medium contains peptone 5g, rhamnose 5g, inositol 5g, sorbitol 18g, peptone 20g, agar 15g, No. 3 bile salt 1.5 g. Neutral red 0.03g, crystal violet 1mg, potassium tellurite 2.5mg, cefixime 0.05mg, 5-bromo-4-chloro-3-indole-β-galactoside 0.02g.

Embodiment 2

[0036] This embodiment provides a kind of separation culture medium that detects Escherichia coli O157, every 1000mL medium contains peptone 2g, rhamnose 2g, inositol 2g, sorbitol 10g, peptone 20g, agar 15g, No. 3 bile salt 1.5 g. Neutral red 0.03g, crystal violet 1mg, potassium tellurite 2.5mg, cefixime 0.05mg, 5-bromo-4-chloro-3-indole-β-galactoside 0.01g.

Embodiment 3

[0038] This embodiment provides a kind of separation culture medium that detects Escherichia coli O157, every 1000mL medium contains peptone 9g, rhamnose 9g, inositol 9g, sorbitol 15g, peptone 20g, agar 15g, No. 3 bile salt 1.5 g. Neutral red 0.03g, crystal violet 1mg, potassium tellurite 2.5mg, cefixime 0.05mg, 5-bromo-4-chloro-3-indole-β-galactoside 0.015g.

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Abstract

The invention provides an isolation medium for detecting Escherichia coli O157 and a preparation method of the isolation medium. The separation medium for detecting the escherichia coli O157 comprises peptone, rhamnose, inositol, sorbitol, peptone, agar, cholate 3, neutral red, crystal violet, potassium tellurite, cefixime and a [beta]-galactosidase chromogenic substrate. The peptone, the rhamnose, the inositol, the sorbitol and the 5-bromo-4-chloro-3-indole-[beta]-galactoside are added into the separation medium provided by the invention, and the content of each component is optimized, so that not only is the false positive rate reduced, but also the Escherichia coli O157:H7 can be quickly distinguished through the color difference, and the specificity of the culture medium is improved, and the cost is not obviously increased.

Description

technical field [0001] The invention relates to the technical field of microorganism detection, in particular to an isolation medium for detecting Escherichia coli O157 and a preparation method thereof. Background technique [0002] Escherichia coli O157:H7 / HM is a foodborne pathogen that seriously endangers human health and can cause non-bloody diarrhea, hemorrhagic colitis, hemolytic uremic syndrome, and even death. It is also the most important pathogenic serotype of enterohemorrhagic or Shiga toxin-producing Escherichia coli (EHEC or STEC). Escherichia coli O157:H7 / HM can infect a variety of foods such as meat, vegetables, milk, juice and drinking water, and has caused food safety problems in many countries around the world. Therefore, Escherichia coli O157:H7 / HM Testing is getting more and more attention. With the continuous development of immunology, biochemistry, and molecular biology, many inspection methods such as magnetic bead enrichment, immunoassay, and DNA pr...

Claims

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Application Information

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IPC IPC(8): C12Q1/10
CPCC12Q1/10C12Q1/045
Inventor 蒋波李琼琼杨美成秦峰戴佳丽张芝华
Owner SHANGHAI INST FOR FOOD & DRUG CONTROL
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