Preparation method of coffee high-branched-chain amino acid protein product
An amino acid and protein technology, applied in protein food processing, protein food ingredients, plant protein processing, etc., can solve problems such as protein imbalance, insufficient cell protein recycling, body protein deficiency, etc.
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Embodiment 1
[0023] 1. Weigh 100kg of corn gluten powder and 15kg of coffee bean powder;
[0024] 2. The corn protein and coffee bean powder raw materials are mixed with 8-10 times of water, and 1100kg of water is added in this experiment;
[0025] 3. Ultra-high temperature sterilization and protein denaturation treatment are carried out on the feed liquid after mixing, and the temperature is at least 135°C for more than 50 seconds;
[0026] 4. Perform cooling treatment to reduce the temperature of the feed liquid to between 54°C and 56°C;
[0027] 5. Add 0.3%-0.5% compound protease by weight of raw materials for enzymolysis treatment. In this example, add 0.35 kg compound protease, and the enzymolysis time is 68 hours. The enzymolysis process does not need to adjust the pH value, but slow stirring is required;
[0028] 6. After centrifuging the enzymatic hydrolysis feed solution to remove the insoluble matter, subject the feed liquid to inactivating enzyme treatment or inactivating enzym...
example 2
[0032] 1. Corn gluten powder 200kg, coffee bean powder 30kg;
[0033] 2. The two raw materials are mixed with 8-10 times of water, and 2200kg of water is added in this experiment;
[0034] 3. Ultra-high temperature sterilization, passivation of physiologically harmful substances and protein denaturation treatment are carried out on the feed liquid after mixing, and the temperature is at least 135°C for more than 50 seconds;
[0035] 4. Perform cooling treatment to reduce the temperature of the feed liquid to between 54°C and 56°C;
[0036] 5. Add 0.3%-0.5% compound protease by weight of raw materials for enzymolysis treatment. In this example, add 0.7 kg compound protease, and the enzymolysis time is 68 hours. The enzymolysis process does not need to adjust the pH value, but slow stirring is required;
[0037] 6. Centrifuge the enzymatic hydrolysis feed solution to remove the insoluble matter, and then subject the feed liquid to 90°C for 30 seconds to inactivate or inactivate...
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