Fish vaccine combined immunization inoculation method

Abstract

The invention provides a fish vaccine combined immunization inoculation method. A first mode is characterized in that vaccine preparation and injection are performed according to the antigen concentration of a late pure edwardsiella attenuated candidate vaccine strain WED and a turbot vibrio anguillarum genetic engineering live vaccine MVAV6203 according to the ratio of 1: 10 to inoculate turbots; and a second mode is characterized in that the turbot in the fry stage is selected, and is soaked in and inoculated with the lagged pure edwardsiella attenuated candidate vaccine strain WED strain, and the turbot vibrio anguillarum genetic engineering live vaccine MVAV6203 strain is inoculated by injection when the turbot grows to the juvenile fish stage. According to the invention, a cold water flounder culture variety is used as an immune target animal, a combined inoculation strategy of the two vaccines is explored, and the feasibility and effectiveness of the combined inoculation strategy are determined by evaluating the influence of the strategy on key production performances such as feed conversion rate, death and culling rate and the like of flounder in actual production. Finally, a novel flatfish healthy breeding production system with combined immunization as the core is established, and the combined prevention and combined control effect on multi-pathogen mixed infection is achieved.

Description

technical field [0001] The invention relates to the field of fish vaccines; in particular, it relates to a combined immunization method of fish vaccines. Background technique [0002] Due to the complex environment of aquaculture, there is a common risk of mixed infection of various pathogens during the aquaculture process. At present, there are only 7 kinds of fish vaccines that have been approved for new veterinary drug registration certificates in my country: inactivated grass carp hemorrhagic disease vaccine, grass carp hemorrhagic disease live vaccine (GCHV-892 strain), inactivated Aeromonas hydrophila septicemia vaccine, flounder Vibrio alginolyticus, Vibrio anguillarum, Edwardsiella tarda multiplex anti-idiotypic antibody vaccine, Edwardsiella tarda live vaccine (EIBAV1 strain), turbot Vibrio anguli genetically engineered live vaccine (MVAV6203 strain ), inactivated vaccine of mandarin fish infectious spleen and kidney necrosis (NH0618 strain), the above-mentioned vacc...

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Problems solved by technology

However, this study did not investigate the content, ratio, and sequence of the two antigens in the implementation of combined vaccination.

[0008] On the other hand, in aquaculture production, turbot seedlings are more likely to be infected with Edwardsiella, and at this stage (<120 days old, <50g) it is not suitable to use large-scale injection inoculation for immunization, onl

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