Microfluidic device and method for simply and rapidly distinguishing cold infection types

Abstract

The invention discloses a microfluidic device and a method for simply and rapidly distinguishing cold infection types, and particularly relates to the technical field of molecular detection and immunodetection of in vitro diagnos. The microfluidic device comprises a shell, a substrate is arranged in the shell, and a sample cavity, two liquid path through holes, a first cavity and a second cavity are formed in the substrate; the liquid path through hole is formed in one side of the sample cavity, the liquid path through hole, the sample cavity and a filter screen are adopted to form a sample filtering device, the first cavity and the second cavity are formed in one side of the liquid path through hole, and manual push rods are arranged in the first cavity and the second cavity. In-vitro diagnosis of diseases is carried out by combining a micro-fluidic chip technology and an immunodiagnosis technology; samples and reagents are uniformly mixed through sample filtration and pretreatment, so that the purity of the samples is improved, and the detection rate is further improved; semi-automation of pretreatment of uniformly mixed samples is realized through an elastic mechanism, quantitative transmission of reagents is realized through the microfluidic structure and the internal circulation system, and the accuracy is improved.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic molecular detection technology and immune detection technology, in particular to a microfluidic device and method for simply and quickly distinguishing cold infection types. Background technique [0002] Microfluidics is a technology for precisely controlling and manipulating micro-scale fluids. It integrates basic operating units such as sample preparation, reaction, separation, and detection in biological, chemical, and medical analysis processes into a small microfluidic chip to complete Technology for analyzing the whole process. Immunochromatography is divided into longitudinal chromatography and lateral chromatography. As a stable and practical technology, immunological lateral flow diagnostic technology is suitable for various instant tests or on-site use. According to the labeling part, it can be divided into colloidal gold immunological lateral flow method, ordinary fluorescence immuno...

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Problems solved by technology

[0004] To this end, the present invention provides a simple and rapid microfluidic device and method for distinguishing the type of cold infection, through the combination of molecular diagnosis and immunodiagnostic detection technology for in vitro diagnosis of diseases; samples can be pretreated to improve the purity of samples, Further improve the detection rate; through the microfluidic structure and internal circulation system, the quantitative transmission of reagents can be realized to improve the accuracy, so as to solve the problems of high cost and low accuracy in the prior art due to the detection being easily interfered

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