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Transmembrane drug delivery method based on bio-enzyme driven micron pump

A biological enzyme and drug technology, applied in the field of drug research, can solve problems such as barriers to transmembrane delivery, affect biomedical efficacy, and low effective cell release rate, and achieve the effect of promoting efficient entry and efficient transmembrane delivery

Pending Publication Date: 2021-08-31
HARBIN INST OF TECH SHENZHEN GRADUATE SCHOOL
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the shortcomings of biomedical nanocarriers mainly exist in the following aspects: (1) Due to the loading mode of surface adsorption or chemical bonding, the loading rate of the load (that is, the ratio of the load to the nanocarrier material) is relatively fixed. The internal effective release rate is generally low; (2) The loading method may have a negative impact on the biomedical functional properties of the load. For example, after the release of a drug linked by a chemical bond, the residual binding group on the molecular structure will affect the The original physicochemical properties and corresponding pharmacokinetic properties, and the functional therapeutic proteins and genes loaded by electrostatic adsorption also have the problem of protein denaturation and gene degradation during the adsorption and desorption process, which affects their biomedical efficacy; ( 3) After entering the cell by phagocytosis, the process of nanocarriers escaping from the inclusion body and entering the cytoplasm is a new barrier to transmembrane transmission

Method used

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  • Transmembrane drug delivery method based on bio-enzyme driven micron pump
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  • Transmembrane drug delivery method based on bio-enzyme driven micron pump

Examples

Experimental program
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Effect test

Embodiment 1

[0029] A transmembrane drug delivery method based on a bioenzyme-driven micropump, comprising:

[0030] (1) Preparation of biomimetic self-driven micropumps: select silica microtubes, activate the microtubes with glutaraldehyde and modify the surface of the microtubes with urease to prepare microenzyme pumps.

[0031] Specifically: using polycarbonate film as a template, using ultrapure water, APTES, TEOA and TEOS to prepare silica microtubes with a diameter of 1 μm and a length of 20 μm. Add 60mg TEOA to 24mL deionized water, stir magnetically at 500rpm; add 30μL APTES to the system, stir at 500rpm for 30min, and raise the temperature of the system to 80°C; finally add 240μLTEOS and polycarbonate membrane to the above system, at 200rpm , stirred at 80°C for 4 hours. After the reaction is over, use alumina particles to polish the silica on the surface of the membrane. After the polishing is complete, rinse the alumina particles with ultrapure water; use DMF to dissolve the po...

Embodiment 2

[0036]Using a transmembrane drug delivery method based on a biological enzyme-driven micropump in Example 1, after successfully establishing the artificial transmembrane channel of the biomimetic micropump, by comparing the transmembrane drug propidium iodide (PI) with / without urea Membrane delivery, to verify the auxiliary effect of the micron enzyme pump on drug delivery. Specific as figure 2 As shown, the present invention promotes the efficient entry of extracellular substances into cells with the assistance of the micro-nano flow field generated by the catalyzed decomposition of urea by urease.

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Abstract

The invention belongs to the technical field of drug research, and particularly relates to a transmembrane drug delivery method based on a bio-enzyme driven micron pump. According to the transmembrane drug delivery method, a one-dimensional nanotube is used as a physical channel for transmembrane substance transfer, an artificial micro-nano machine, namely a biological nano pump, is embedded into the surface of a cell membrane, an energy source is provided by utilizing a biocompatible bio-enzyme catalytic reaction, an inner flow field of the nanotube is driven to move, extracellular substances are transferred into cells, and therefore, a novel pumping type transmembrane substance transfer mode is established on the two sides of the cell membrane, and the limitation of a traditional nano-drug carrier is expected to be broken through.

Description

technical field [0001] The invention belongs to the technical field of drug research, and in particular relates to a transmembrane drug delivery method based on a bioenzyme-driven micropump. Background technique [0002] Traditional chemotherapy has the disadvantages of poor specificity and strong side effects in the treatment of cancer. Therefore, nanomedicine methods that can improve specificity and reduce side effects have been extensively studied. In nanotherapy, nanomedicine mainly relies on endocytosis of cells to enter cells. The drug is loaded on the nanocarrier, the cell membrane is depressed, and the nanocarrier loaded with the drug is wrapped to form an inclusion body and enters the cell. Afterwards, the nanocarrier escapes from the inclusion body and enters the cytoplasm, releasing the drug in the cytoplasm for treatment. [0003] At present, the shortcomings of biomedical nanocarriers mainly exist in the following aspects: (1) Due to the loading mode of surface...

Claims

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Application Information

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IPC IPC(8): A61K47/62A61K47/02A61K47/46A61K31/473C12N5/09
CPCA61K47/62A61K47/46A61K47/02A61K31/473C12N5/0693C12N5/0682
Inventor 马星郭佩婷王丽英
Owner HARBIN INST OF TECH SHENZHEN GRADUATE SCHOOL
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