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Degradable hydrogels for pulp dentin regeneration

A hydrogel and dentin technology, applied in the field of medicine, can solve the problems of poor proliferation and limited application of dental pulp stem cells

Active Publication Date: 2021-11-02
BEIJING STOMATOLOGY HOSPITAL CAPITAL MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unfortunately, the proliferation of dental pulp stem cells in this hydrogel is poor, which limits its application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: Preparation of degradable hydrogel

[0023] (1) Dissolve chitosan in aqueous acetic acid solution to obtain a 3w / w% chitosan solution, and dissolve di-4-formylbenzoic acid polyethylene glycol (DF-PEG) in deionized water to obtain a 20w / w % aqueous solution, add 25ml DF-PEG aqueous solution to 70ml chitosan solution, then add 0.1mg epidermal growth factor (EGF) and 0.2mg interleukin-2, stir and react at room temperature for 40 seconds to obtain a hydrogel;

[0024] (2) Adult dental pulp cells were cultured by tissue block method, trypsinized and passaged, and the dental pulp cells passed to the fifth passage were trypsinized to collect the cells, and the cell suspension was 5.0×10 6 cells / g gel inoculation, 37°C, CO 2 Box overnight, use the next day, test the number of viable dental pulp cells in the gel before use, and detect by cell counting method, which contains 5.8×10 6 unit / g gel.

Embodiment 2

[0026] Using the same preparation as in Example 1, the difference is that the epidermal growth factor (EGF) is 0.2 mg, and the interleukin-2 is 0.1 mg. The number of viable dental pulp cells in the test gel before use is detected by cell counting. Which contains 4.2×10 6 unit / g gel.

Embodiment 3

[0028] Using the same preparation as in Example 1, the difference is that the epidermal growth factor (EGF) is 0.2 mg, and the interleukin-2 is 0.2 mg. The number of viable dental pulp cells in the test gel before use is detected by cell counting. Which contains 4.6×10 6 unit / g gel.

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PUM

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Abstract

The invention relates to a degradable hydrogel for pulp and / or dentin regeneration, which is characterized in that the hydrogel uses the reactant of chitosan and di-4-formyl benzoic acid polyethylene glycol ester as a scaffold material, the hydrogel also contains epidermal growth factor (EGF) and interleukin‑2. The hydrogel of the present invention has a self-repair function, and can be conveniently injected into the pulp cavity that needs to be treated. Through the synergistic effect of epidermal growth factor (EGF), interleukin-2 and scaffold materials, the repair cells enter the dental pulp cavity and play a restorative role, thereby regenerating and repairing diseased pulp and dentin.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a degradable hydrogel for pulp dentin regeneration and application thereof. Background technique [0002] Most of the biological activity of the tooth mainly depends on the pulp. Currently, covering or replacing it with an inert synthetic material, i.e. root canal therapy, is the main way to treat pulp diseases. The more common filling material is Eucommia gum, which is an isoprene thermoplastic polymer. For treatment, after the dead pulp is removed, molten hot gutta-percha is injected into the root canal. Although root canal therapy has been widely used in dental treatment in recent years, there are still the following disadvantages: the teeth after root canal therapy are more brittle and easy to break; teeth after root canal therapy frequently discolor, and the cost of later dental cosmetic surgery is high ; There is no root canal treatment for diseased teeth in ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/38A61L27/18A61L27/20A61L27/22A61L27/50A61L27/52A61L27/54A61L27/58
CPCA61L27/18A61L27/20A61L27/227A61L27/3804A61L27/3865A61L27/50A61L27/52A61L27/54A61L27/58A61L2300/252A61L2300/412A61L2300/414A61L2400/06A61L2430/12C08L5/08C08L71/02
Inventor 周建王玉记王变红魏雪竺朱梦迪夏登胜赵翔
Owner BEIJING STOMATOLOGY HOSPITAL CAPITAL MEDICAL UNIV
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