Artificial propagation method of Ptychobarbus kaznakovi

A technology for artificial reproduction and baleen fish, which is applied to the field of artificial breeding of naked-bellied baleen fish, can solve the problems of blank stage and other aspects of baleen baleen fish, especially the related research on artificial breeding technology, and the like. Solve the effect of decreasing, simple operation and protecting ecological resources

Pending Publication Date: 2021-09-03
武汉中科瑞华生态科技股份有限公司 +1
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AI Technical Summary

Problems solved by technology

[0003] Regarding the research work on the naked-bellied phyllid, some scholars first carried out classification and distribution studies, such as Wu Yunfei, Wu Cuizhen (Qinghai-Tibet Plateau Fish, Sichuan Science and Technology Press, 1991.415-419), Tibet Autonomous Region Fisheries Bureau (Tibetan Fish Species and their resources, China Agricultural Press, 1995.103-109); some scholars have conducted research on the age and growth of phyllotrichia, such as Li Fei, Yang Dedong, He Yongfeng, Yao Dedong, Yao Jianwei, Zhu Tingbing (gifted to Qu Bare The age and growth of phyllids, Freshwater Fisheries, 2016 (06)), Li Zhao, Zhu Fengyue, Liu Mingdian, Wang Qi, Liu Shaoping, Duan Xinbin (Age structure and growth characteristics of phyllids in the upper reaches of t

Method used

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  • Artificial propagation method of Ptychobarbus kaznakovi

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Experimental program
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Embodiment 1

[0027] Select the wild phyllophyllus with no wounds on the body surface and weigh 400-900g as the broodstock for breeding, and put the selected broodstock in a circular glass tank for cultivation with a circulating aquaculture system, with a breeding density of 3.5kg / m 3 , adding Bacillus subtilis to the circulating water to control the water quality, the dissolved oxygen in the water is 7mg / L, the pH of the water is 8.0, the water flow is 18L / min, the water temperature is 4-15℃, the feed used in the cultivation process is protein content of 36% of the Haida special fish compound feed, the daily feeding amount is 0.5% of the broodstock body weight, and it is fed once a day at 9:00 am and 6:00 pm respectively; 2 pairs of healthy and healthy mature phyllids are used as parents for oxytocin, and the mature phyllids are placed in a circular oxytocin pool at a density of 3kg / m 3, the water flow rate is 5L / min, the water temperature in the oxytocin pool is controlled at 9°C, the dis...

Embodiment 2

[0030] Select the wild phyllocarpus with no wounds on the body surface and weigh 700-1300g as the broodstock for breeding, and put the selected broodstock in a circular glass tank for cultivation with a circulating aquaculture system at a breeding density of 3.5kg / m 3 , adding Bacillus subtilis to the circulating water to control the water quality, the pH value of the water is 7.8, the water flow rate is 16L / min, the dissolved oxygen in the water body is 7mg / L, the water temperature is 13°C, and the feed used in the daily cultivation process is a protein content of 41 % of Haida special fish compound feed, the daily feeding amount is 0.8% of the broodstock body weight, fed once a day at 9:00 am and 6:00 pm respectively, and 0.2% of the broodstock body weight is fed daily during the later cultivation Water earthworms; in May, 10 pairs of disease-free and healthy mature phyllids were selected from the recirculating aquaculture system as parents for oxytocin, and the mature phylli...

Embodiment 3

[0033] Select the wild phyllophyllus with no wounds on the body surface and weigh 600-1100g as the broodstock for cultivation, and put the selected broodstock in a circular glass tank for cultivation with a circulating aquaculture system with a breeding density of 3.5kg / m 3 , add Bacillus subtilis to the circulating water to control the water quality, the pH value of the water is 8.0, the water flow rate is 17L / min, the water temperature is 16°C, the dissolved oxygen in the water body is 9mg / L, and the feed used in the daily cultivation process is protein content of 40 % of Haida special fish compound feed, the daily feeding amount is 0.6% of the broodstock body weight, fed once a day at 9:00 am and 6:00 pm respectively, and 0.4% of the broodstock body weight is fed daily during the later cultivation Water earthworms; in May, 4 pairs of disease-free and healthy mature phyllids were selected from the recirculating aquaculture system as parents for oxytocin, and the mature phylli...

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Abstract

The invention provides an artificial propagation method of Ptychobarbus kaznakovi, which comprises the following steps: parent fish rearing: selecting wild Ptychobarbus kaznakovi with no body surface injury and body weight of 400-1300 g as parent fish for rearing, wherein in the rearing process, the daily feeding amount of feed is 0.1-1% of the body weight of the parent fish; parent fish selection: selecting disease-free and robust mature Ptychobarbus kaznakovi as a spawning induction parent; artificial spawning induction: firstly, only injecting a first spawning induction agent into female fish in spawning induction parents for compatibility; after 24 h, continuously injecting a second oxytocic into the female fish for compatibility, and injecting a third oxytocic into the male fish in the oxytocic parents for compatibility; artificial insemination: temporarily rearing the induced spawning parents in an induced spawning pond at the water temperature of 9-14 DEG C, and checking female fishes in the induced spawning parents once every 8 h after 48 h; pressing the belly of the female fish slightly, if roes are discharged, squeezing the roes into a basin, then squeezing the semen of the male fish into the basin, and conducting stirring, standing and cleaning to obtain fertilized roes; and hatching: hatching fertilized roes obtained by using flowing water, and obtaining the Ptychobarbus kaznakovi fry after the hatching is completed.

Description

technical field [0001] The invention belongs to the technical field of fish reproduction, and in particular relates to an artificial reproduction method of phyllotrichia niguri. Background technique [0002] Ptychobarbus kaznakovi Nikolsky, scientific name: Ptychobarbus kaznakovi Nikolsky, is a fish of the genus Ptychobarbus Cyprinidae of Cyprinidae, commonly known as flower fish, and is a unique species in China. Naked bellyfish is distributed in the upper reaches of the Jinsha River, Lancang River, and Nujiang River, etc., and generally lives in the flowing water environment of the main and tributary streams. In recent years, due to high-intensity fishing, the amount of fishing far exceeds the amount of natural reproduction, changes in the ecological environment, water pollution and other reasons, resulting in a sharp decline in the population. It has been basically extinct in some river sections, and there are only a small number of individuals in remote upstream areas. ...

Claims

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Application Information

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IPC IPC(8): A01K61/10A61D7/00
CPCA01K61/10A61D7/00Y02A40/81
Inventor 佘洪李赞谭冬明施龙王鑫魏再强邓俊强舒敏冉玉凤关景春丁瑶何兴恒张海松谭德清陈启超
Owner 武汉中科瑞华生态科技股份有限公司
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