Red-green-blue fluorescence emission molecularly imprinted sensor, and preparation method and application thereof
A molecular imprinting and sensor technology used in analytical chemistry and rapid detection
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Embodiment 1
[0055] Preparation of red-green-blue fluorescent emission molecularly imprinted sensor:
[0056] Take folic acid with auto-blue fluorescence as an example for blotting template:
[0057] (1) Prepare green fluorescent folic acid imprinted microspheres and red fluorescent folic acid imprinted microspheres, see figure 1 (i):
[0058] Preparation of green fluorescent folic acid imprinted microspheres (g-MIPs): 1mL SiO 2 Nanoparticles (10 mg / mL) were dispersed in 16 mL of ultrapure water, 8 mg of folic acid and 37 μL of APTES were added, after stirring for 1 h, 3 mL of green fluorescent cadmium telluride quantum dots (g-QDs) were added, and 50 μL of ammonia (NH 3 ·H 2 O) and 50μL tetraethyl orthosilicate (TEOS), react in the dark for 12h. Finally, the products (g-MIPs) were collected by centrifugation, and after the template was eluted with methanol, they were dispersed in 20 mL of ultrapure water for future use. The green fluorescent folic acid imprinted microspheres (g-MIPs)...
Embodiment 2
[0069] Mix 50 μL of the obtained green fluorescent folic acid imprinted microspheres (g-MIPs) dispersion with different amounts of the above obtained red fluorescent folic acid imprinted microspheres (r-MIPs) and then uniformly disperse them in 100 μL of phosphate buffer solution (0.1 M, pH 8 .0), then use ultrapure water to dilute the final volume to 1mL to obtain the sensor; wherein, the amount of green fluorescent folic acid imprinted microspheres (g-MIPs) dispersion is fixed at 5% of the total sensor volume (1mL) (i.e. 50 μL), the amount of red fluorescent folic acid imprinted microspheres (r-MIPs) dispersion accounted for 0, 0.5%, 2%, 3.5%, 5%, 8% of the total sensor volume (1mL) respectively (that is, the addition amount was 0, 5, 20, 35, 50, 80μL); then add different amounts of folic acid to the above sensor to make the final concentration reach 0, 0.5, 1, 5, 10, 20, 30, 50ppm, and react for 7 minutes, after excitation The fluorescence spectrum was measured under the co...
Embodiment 3
[0072] According to the sensor preparation method described above, different sensors are prepared, specifically, the different microspheres obtained above are mixed in a certain proportion, that is, different sensors with different selections and different proportions are obtained; among them, g-MIPs in the red-green-blue fluorescence emission molecularly imprinted sensor : r-MIPs = 5%: 3.5% (volume percentage), red-green-blue fluorescence emission g-NIPs in the non-imprinted sensor: r-NIPs = 5%: 3.5% (volume percentage), green-blue double fluorescence emission g-MIPs=5% (volume percentage) in the molecularly imprinted sensor, and r-MIPs=3.5% (volume percentage) in the red-blue dual fluorescence emission molecularly imprinted sensor.
[0073]Add different amounts of folic acid to the different sensors obtained above to make the final concentration reach 0, 0.01, 0.05, 0.1, 0.5, 1, 5, 10, 15, 20, 30, 40 and 50ppm, and react for 7 minutes. The fluorescence spectrum was measured ...
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