Red-green-blue fluorescence emission molecularly imprinted sensor, and preparation method and application thereof

A molecular imprinting and sensor technology used in analytical chemistry and rapid detection

Active Publication Date: 2021-09-03
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, to prepare a red-green-blue fluorescent emission molecularly imprinted sensor for any target that needs to be detected, it is required to find three suitable fluorescent sources at the same time and provide corresponding changes in the appropriate fluorescent signals, such as the increase of a fluorescent peak with the concentration of the target Enhancement and quenching of the other two fluorescence peaks, or quenching of one fluorescence peak and enhancement of the other two fluorescence peaks, which is difficult to achieve

Method used

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  • Red-green-blue fluorescence emission molecularly imprinted sensor, and preparation method and application thereof
  • Red-green-blue fluorescence emission molecularly imprinted sensor, and preparation method and application thereof
  • Red-green-blue fluorescence emission molecularly imprinted sensor, and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0055] Preparation of red-green-blue fluorescent emission molecularly imprinted sensor:

[0056] Take folic acid with auto-blue fluorescence as an example for blotting template:

[0057] (1) Prepare green fluorescent folic acid imprinted microspheres and red fluorescent folic acid imprinted microspheres, see figure 1 (i):

[0058] Preparation of green fluorescent folic acid imprinted microspheres (g-MIPs): 1mL SiO 2 Nanoparticles (10 mg / mL) were dispersed in 16 mL of ultrapure water, 8 mg of folic acid and 37 μL of APTES were added, after stirring for 1 h, 3 mL of green fluorescent cadmium telluride quantum dots (g-QDs) were added, and 50 μL of ammonia (NH 3 ·H 2 O) and 50μL tetraethyl orthosilicate (TEOS), react in the dark for 12h. Finally, the products (g-MIPs) were collected by centrifugation, and after the template was eluted with methanol, they were dispersed in 20 mL of ultrapure water for future use. The green fluorescent folic acid imprinted microspheres (g-MIPs)...

Embodiment 2

[0069] Mix 50 μL of the obtained green fluorescent folic acid imprinted microspheres (g-MIPs) dispersion with different amounts of the above obtained red fluorescent folic acid imprinted microspheres (r-MIPs) and then uniformly disperse them in 100 μL of phosphate buffer solution (0.1 M, pH 8 .0), then use ultrapure water to dilute the final volume to 1mL to obtain the sensor; wherein, the amount of green fluorescent folic acid imprinted microspheres (g-MIPs) dispersion is fixed at 5% of the total sensor volume (1mL) (i.e. 50 μL), the amount of red fluorescent folic acid imprinted microspheres (r-MIPs) dispersion accounted for 0, 0.5%, 2%, 3.5%, 5%, 8% of the total sensor volume (1mL) respectively (that is, the addition amount was 0, 5, 20, 35, 50, 80μL); then add different amounts of folic acid to the above sensor to make the final concentration reach 0, 0.5, 1, 5, 10, 20, 30, 50ppm, and react for 7 minutes, after excitation The fluorescence spectrum was measured under the co...

Embodiment 3

[0072] According to the sensor preparation method described above, different sensors are prepared, specifically, the different microspheres obtained above are mixed in a certain proportion, that is, different sensors with different selections and different proportions are obtained; among them, g-MIPs in the red-green-blue fluorescence emission molecularly imprinted sensor : r-MIPs = 5%: 3.5% (volume percentage), red-green-blue fluorescence emission g-NIPs in the non-imprinted sensor: r-NIPs = 5%: 3.5% (volume percentage), green-blue double fluorescence emission g-MIPs=5% (volume percentage) in the molecularly imprinted sensor, and r-MIPs=3.5% (volume percentage) in the red-blue dual fluorescence emission molecularly imprinted sensor.

[0073]Add different amounts of folic acid to the different sensors obtained above to make the final concentration reach 0, 0.01, 0.05, 0.1, 0.5, 1, 5, 10, 15, 20, 30, 40 and 50ppm, and react for 7 minutes. The fluorescence spectrum was measured ...

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Abstract

The invention belongs to the field of analytical chemistry and rapid detection, and particularly relates to a red-green-blue fluorescence emission molecularly imprinted sensor which takes a fluorescent target object as an imprinting template and is based on a post-imprinting mixing method, a preparation method thereof, and application of the molecularly imprinted sensor in accurate and visual detection of the fluorescent target object. The red-green-blue fluorescence emission molecularly imprinted sensor is obtained by taking the target object with any one fluorescence color in red, green and blue as the imprinting template, respectively synthesizing imprinting microspheres containing imprinting holes and embedding fluorescence sources of the other two colors, and then mixing the two fluorescent imprinting microspheres by a post-imprinting mixing method. The sensor prepared by the method disclosed by the invention can detect a fluorescent target object in a high-sensitivity, high-selectivity and self-correcting manner, the provided fluorescence color change is wider and richer than that of a traditional double-fluorescence-emission molecular imprinting sensor, and more accurate naked-eye visual detection can be performed on the fluorescent target object.

Description

technical field [0001] The invention belongs to the field of analytical chemistry and rapid detection, and in particular relates to a red-green-blue fluorescent emission molecular imprinting sensor using a fluorescent target as an imprinting template and based on a post-imprinting mixing method and its preparation and accurate visual detection of fluorescent target substances Applications. Background technique [0002] Nowadays, rapid visual detection plays an important role in many fields such as environmental detection, food safety, and clinical diagnosis. The rapid visual detection of a large number of samples to be tested, combined with the precise detection of suspicious samples with large-scale instruments (such as high-performance liquid chromatography) often constitute a complete detection process, which not only saves money and time spent on detection, but also ensures the reliability of detection results. Therefore, rapid visual detection methods are often require...

Claims

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Application Information

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IPC IPC(8): C09K11/02G01N21/64
CPCC09K11/02G01N21/6428G01N2021/6432
Inventor 李金花杨倩李楚瑶陈令新
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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