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Method for extracting tremella polysaccharide by using deep eutectic solvent

A technology of deep eutectic solvent and tremella polysaccharide, which is applied in the field of bioengineering to achieve high extraction rate, high product purity and reduce environmental pollution

Inactive Publication Date: 2021-09-07
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the study of extracting Tremella polysaccharide from Tremella spore fermentation liquid by using deep eutectic solvent has not been reported yet.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A method utilizing deep eutectic solvent to extract tremella polysaccharide, comprising the following steps:

[0043] Step 1: Mix hydrogen bond acceptor glycerol, hydrogen bond donor choline chloride and deionized water at a mass ratio of 1:1:40, stir in a water bath at 80°C until clear and transparent, and the solution is crystal-free after standing for a period of time Precipitate, obtain deep eutectic solvent;

[0044] Step 2: Mix the freeze-dried tremella spore fermentation liquid with the deep eutectic solvent prepared in step 1 at a ratio of 1g:10mL; then extract the mixture in a water bath at 80°C, and centrifuge to obtain tremella polysaccharide extract;

[0045] Step 3: Add ethanol with a volume concentration of 95% to the tremella polysaccharide extract, the volume ratio of the tremella polysaccharide extract to ethanol is 1:4, mix well, let stand, and centrifuge, take the precipitate, wash and centrifuge Finally, the precipitate was added to 100 mL of distil...

Embodiment 2

[0050] A method utilizing deep eutectic solvent to extract tremella polysaccharide, comprising the following steps:

[0051] Step 1: Mix hydrogen bond acceptor glycerol, hydrogen bond donor choline chloride and deionized water at a mass ratio of 1:2:80, stir in a water bath at 80°C until clear and transparent, and the solution is crystal-free after standing for a period of time Precipitate, obtain deep eutectic solvent;

[0052] Step 2: Mix the freeze-dried tremella spore fermentation liquid with the deep eutectic solvent prepared in step 1 at a ratio of 1g:30mL; then extract the mixture in a water bath at 80°C, and centrifuge to obtain tremella polysaccharide extract;

[0053] Step 3: Add ethanol with a volume concentration of 95% to the tremella polysaccharide extract, the volume ratio of the tremella polysaccharide extract to ethanol is 1:4, mix well, let stand, and centrifuge, take the precipitate, wash and centrifuge Finally, the precipitate was added to 100 mL of distil...

Embodiment 3

[0058] A method utilizing deep eutectic solvent to extract tremella polysaccharide, comprising the following steps:

[0059] Step 1: Mix hydrogen bond acceptor glycerol, hydrogen bond donor choline chloride and deionized water at a mass ratio of 1:3:80, stir in a water bath at 80°C until clear and transparent, and the solution is crystal-free after standing for a period of time Precipitate, obtain deep eutectic solvent;

[0060] Step 2: Mix the freeze-dried tremella spore fermentation broth with the deep eutectic solvent prepared in step 1 at a ratio of 1g:50mL; then extract the mixture in a water bath at 80°C and centrifuge to obtain tremella polysaccharide extract;

[0061] Step 3: Add ethanol with a volume concentration of 95% to the tremella polysaccharide extract, the volume ratio of the tremella polysaccharide extract to ethanol is 1:4, mix well, let stand, and centrifuge, take the precipitate, wash and centrifuge Finally, the precipitate was added to 100 mL of distille...

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PUM

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Abstract

The invention discloses a method for extracting tremella polysaccharide by using a deep eutectic solvent. The extraction method comprises the following steps: mixing freeze-dried tremella spore fermentation liquor with the deep eutectic solvent; performing water bath extraction on the mixture, and centrifuging to obtain tremella polysaccharide extract; and adding a solvent into the tremella polysaccharide leach liquor, uniformly mixing, standing, centrifuging, and washing, centrifuging and freeze-drying the precipitate to obtain the tremella polysaccharide. The deep eutectic solvent comprises a hydrogen bond donor, a hydrogen bond acceptor and deionized water, the hydrogen bond donor is glycerol, and the hydrogen bond acceptor is choline chloride. According to the method, the tremella polysaccharide is extracted by utilizing a deep eutectic solvent method, the deep eutectic solvent synthesis method is characterized in that choline chloride and glycerol are heated and then are combined together through hydrogen bonds to form the deep eutectic solvent, and the deep eutectic solvent is used as a solvent for extracting the tremella polysaccharide in the tremella spore fermentation liquor, so that the method has the advantages of high operability, simple and convenient process and low cost; meanwhile, the raw materials are easy to obtain, renewable, degradable and recoverable.

Description

technical field [0001] The invention relates to a method for extracting tremella polysaccharide by using a deep eutectic solvent, belonging to the technical field of bioengineering. Background technique [0002] Polysaccharides are high-molecular polymers composed of tens of thousands of monosaccharides linked by glycosidic bonds. Polysaccharides are widely distributed in nature and exist in plants, animals, and microorganisms. Natural polysaccharides can be used in clinical trials, such as the treatment of diseases such as anti-diabetes, anti-tumor, and hypolipidemic, or as anti-inflammatory and anti-viral drugs. Polysaccharides play an important role in maintaining the normal operation of the human body. [0003] Tremella fuciformis, also known as "Tremella fuciformis", belongs to the order Tremella and Tremellaceae, and is an edible fungus. Tremella polysaccharide is the main active ingredient, which is mainly composed of mannose, glucuronic acid, glucose, galactose, xy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/006C08B37/0003
Inventor 马霞魏雅姝何艳
Owner SHANGHAI INST OF TECH