Method for improving metabolic capability of bacillus amyloliquefaciens and activity of producing alpha-amylase by adopting vacuum ultraviolet radio frequency plasma

Abstract

The invention belongs to the field of bacterial strain activity improvement, and relates to a method for improving the metabolic capability of bacillus amyloliquefaciens and the activity of producing alpha-amylase by adopting vacuum ultraviolet radio frequency plasma, which comprises the following steps that an LB (Luria-Bertani) plate full of the bacillus amyloliquefaciens is put into cold plasma processing equipment, the strain generates energy level transition after being radiated by vacuum ultraviolet photons in a cold plasma environment, the cold plasma processing equipment takes helium as a cold plasma gas source, and the helium generates helium high-energy plasma and vacuum ultraviolet photons through radio frequency discharge. The whole process is free of heating, low-temperature pipeline production is achieved, mutagenesis is not generated on strain molecules, active molecular structures are not affected, only energy level changes of the molecules are targeted, the treatment effect is high, inoculated fermentation can be directly conducted after treatment is finished, the tedious bacterium screening process is abandoned, the production period is greatly shortened, the production process is simplified, and the production cost is reduced, so that manpower, material resources and time cost of companies and enterprises can be effectively saved.

Description

Technical field [0001] The present invention relates to a vacuum ultraviolet RF plasma Bacillus amyloliquefaciens enzymatic activity and the metabolic capacity of the starch solution improved yield α-, strains belonging to the field of improved activity. Background technique [0002] Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) is a bacterial gram positive and spore staining leather. Presenting a short rod under a microscope (0.7 ~ 3.0μm), the optimum temperature is 28 ~ 30 ℃, pH optimum growth 6.5 to 7.0. Colonies on solid medium primarily as a light yellow, opaque, irregular edges and rough surface. In the early detection, it is possible due to the ability to ferment and produce amylase was named Bacillus amyloliquefaciens. Amyloliquefaciens food commonly found in a variety of environments, plants, soil, etc., with a strong value. During the fermentation process, Bacillus amyloliquefaciens metabolites can effectively inhibit the growth of fungi and bacteria, the majo...

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Problems solved by technology

However, this technology also has some problems, such as the plasma energy it produces is low, and the photochemical reaction caused by photon collision cannot occur on the surface of the material. Due to the high lethality rate and low mutation rate, cumbersome bacteria screening process is required after mutagenesis, which will limit the development of related technologies due to heavy workload and long experiment period.

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