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Full-scale C-reactive protein detection kit

A technology for detecting kits and reactive proteins, which can be used in biological testing, measuring devices, material inspection products, etc., and can solve the problems of low-concentration samples with zero values, narrow linear range, and low sensitivity.

Pending Publication Date: 2021-09-07
JILIN GETEIN BIOTECH CO LTD
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Problems solved by technology

[0005] The purpose of the embodiments of the present invention is to propose a new full-range C-reactive protein detection kit to solve the problems of low sensitivity of the current full-range CRP kits on the market, detection of low-concentration samples with zero value and narrow linear range.

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  • Full-scale C-reactive protein detection kit

Examples

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Embodiment 1

[0025] This embodiment provides a C-reactive protein detection kit, which includes reagent R1 and reagent R2.

[0026] Preferably, the reagent R1 includes the following components: 20 mM / L phosphate buffer, 15 g / L sodium chloride; the pH of the reagent R1 is 7.33.

[0027] Preferably, the reagent R2 includes the following components: 50mM / LTris buffer, 0.2mg / mL CRP polyclonal antibody, 0.4mg / mL CRP monoclonal antibody, 0.4%w / v 88nm latex microspheres, 0.2%w / v 180nm latex Microspheres, 1 g / L sodium azide, the pH of the reagent R2 is 7.00.

[0028] Preferably, the volume ratio of the reagent R1 to the reagent R2 is 1:1.

[0029] The performance of the C-reactive protein detection kit obtained in Example 1 was verified on an automatic biochemical analyzer.

[0030] The detection parameters of the kit are shown in Table 1:

[0031] Table 1 Detection parameters of the kit.

[0032]

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Abstract

The invention provides a full-scale C-reactive protein detection kit. Two latex microspheres are respectively coupled with a C-reactive protein polyclonal antibody and a monoclonal antibody to achieve the purpose of enhancing the reagent sensitivity and the detection upper limit. The invention solves the problems of low sensitivity, low detection upper limit and poor stability of common latex immunoturbidimetry. The kit disclosed by the invention comprises a reagent R1 and a reagent R2. The reagent R1 is a proper buffer solution, and the reagent R2 is a solution of latex microspheres coupled with an anti-human CRP antibody. The kit provided by the invention has the characteristics of high sensitivity, high detection upper limit, strong specificity, good stability and the like. The kit can be used for detecting the content of C-reactive protein in human serum or plasma, and is suitable for a full-automatic biochemical analyzer. The sensitivity of the kit can reach 0.01 mg / L, and the detection upper limit reaches 400 mg / L.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnostic biochemical detection, in particular to a full-range C-reactive protein detection kit. Background technique [0002] C-reactive protein (C-reactive protein, CRP) refers to a protein that rises sharply in the plasma when living organisms are infected and traumatized. It increases rapidly several hours after the onset of various acute inflammation, tissue injury, myocardial infarction, surgical trauma, radiation injury and other diseases, and has the tendency of multiplying. When the lesion improves, it quickly drops to normal again, and its increase is positively correlated with the degree of infection. Therefore, C-reactive protein is a very important non-specific inflammatory marker in in vitro diagnosis. [0003] Common detection methods for CRP include colloidal gold method, enzyme-linked immunoassay (ELISA), and latex-enhanced immunoturbidimetric method. Among these methods, lat...

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Application Information

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IPC IPC(8): G01N33/68G01N33/577G01N33/543
CPCG01N33/6893G01N33/577G01N33/54346G01N2333/4737G01N2800/7095
Inventor 刘凯刘玲陈凯丽王旭倪志华
Owner JILIN GETEIN BIOTECH CO LTD
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