Application of dihydromyricetin in drug dependence/addiction resistance
A dihydromyricetin, anti-drug technology, applied in the field of medicine, can solve the problems of methadone dependence, addiction, and insufficient safety
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Embodiment 1
[0020] The dihydromyricetin in this example is dihydromyricetin extracted from Maoyanberry (vine tea). extraction methods such as figure 2 As shown, 50% dihydromyricetin was obtained by filtering and drying, and 98% dihydromyricetin was obtained by recrystallization. Animal experiments were carried out using the DHM extracted by the above method.
[0021] Select 36 male 8-week-old mice and divide them into 6 groups, and use the social separation method to create animal models of anxiety and depression. The 6 groups are divided into 1 group: group feeding for 2 weeks (no drug) + sucrose as blank control feeding for 2 weeks; 2 groups : Group feeding for two weeks (without drug) + DHM (2mg / kg / day) for 2 weeks; Group 3: separate feeding for 2 weeks (without drug) + sucrose as blank control feeding for 2 weeks; Group 4: separate feeding for 2 weeks (no drug)+DHM (2mg / kg / day) feeding for 2 weeks (2mg / kg / day); Group 5: separate feeding for 2 weeks (no drug)+DHM (2mg / kg / day) feedin...
Embodiment 2
[0023] Select 20 male adult SD rats (body weight 200-250g) for one-time intraperitoneal injection of ethanol (20% ethanol solution, 20ml / kg) to establish an alcohol dependence model, and then divide them into two groups: alcohol (20% ethanol solution, 20ml / kg) / kg)+water group and ethanol (20% ethanol solution, 20ml / kg)+DHM group (2mg / kg, dissolve in drinking water), observe the spontaneous alcohol intake of animals between different groups (put in the experimental field 20% alcohol solution, animals can drink themselves). Such as Figure 5 As shown, the alcohol intake in the alcohol + water group increased from 3.9 / kg / day to 7.4 g / kg / day at the end of the second week; while there was no significant change in the alcohol + DHM group. The alcohol + water group was divided into two subgroups at the seventh week, and the DHM 2mg / kg group was given and the original plan was followed. The alcohol intake of the DHM group decreased significantly. Group alcohol intake had been simil...
Embodiment 3
[0025] In this example, dihydromyricetin was synthesized by chemical methods. Animal experiments were carried out using the DHM extracted by the above-mentioned chemical synthesis method.
[0026] Select 42 male mice and divide them into 6 groups: NS (normal saline group), normal saline+morphine group, clonidine+morphine group, morphine+DHM low-middle-high dose group (respectively 1 / 2 / 5mg / kg / day ). Except for the first NS group, the other groups received subcutaneous injection of morphine 50 mg / kg for four consecutive days, and intraperitoneal injection of morphine antagonist naloxone 1 hour after the administration of morphine on the fourth day to establish a morphine withdrawal model; DHM Administered by intraperitoneal injection. One hour after administration of naloxone on the fourth day, the animals were observed for withdrawal symptom-startle. Such as Image 6 As shown, DHM significantly reduced the startle induced by morphine withdrawal.
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