Probe and kit for detecting bordetella parapertussis and use method of kit

A technology of parapertussis and kits, applied in biochemical equipment and methods, microbiological determination/testing, DNA/RNA fragments, etc., which can solve problems such as sensitivity differences and no protective effect

Pending Publication Date: 2021-09-10
国药(武汉)医学实验室有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The acellular pertussis vaccine has little protection against Bordetella parapertussis and Bordetella hallinii, and several Bordetella species have variable susceptibility to different antimicrobial agents

Method used

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  • Probe and kit for detecting bordetella parapertussis and use method of kit
  • Probe and kit for detecting bordetella parapertussis and use method of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0043] A kit for detecting Bordetella parapertussis nucleic acid based on MIRA fluorescence method, the kit is provided with: Abuffer, B buffer, first primer, second primer, fluorescent probe, ddH 2 O, negative quality control material, positive quality control material.

[0044] Among them, referring to the instructions of the magnetic bead method nucleic acid extraction kit, the nucleic acid of parapertussis positive patients was extracted as a positive quality control substance.

[0045] Wherein, the described test kit of the finished product includes the following:

[0046] Reagent name content A buffer 1.6mL×1 tube B buffer 150uL×1 tube Positive control template 100uL×1 tube Positive Control Primer Probe Mix 200uL×1 tube Negative Control Template 100uL×1 tube ddH 2 O

1mL×1 tube

[0047] Wherein, the amplification reaction reagent is divided into a single tube and is in the form of dry powder. The number of re...

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PUM

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Abstract

The invention provides a probe and a kit for detecting bordetella parapertussis and a use method of the kit. The kit is a bordetella parapertussis nucleic acid detection kit based on an MIRA fluorescence method, wherein the primer of the MIRA is designed according to the specific insertion repeat sequence IS1001 of the bordetella parapertussis. The invention provides the nucleic acid detection kit which is rapid, specific and sensitive and does not need complex instruments and equipment and the use method of the nucleic acid detection kit.

Description

technical field [0001] The invention belongs to the technical field of molecular diagnostic biology, and relates to a probe for detection of Bordetella parapertussis, a kit and a use method thereof. Background technique [0002] The genus Bordetella is a gram-negative bacillus, which currently includes 10 species, including B. pertussis, B. parapertussis, and B. pertussis. Bacteria (B. holmessi) and B. bronchiseptica are associated with respiratory tract infections in humans. The only host for the first three is humans. Bordetella pertussis is the main causative agent of pertussis infection in humans, often infecting unvaccinated infants and young children. B. parapertussis usually causes milder pertussis-like symptoms and is of short duration. Bordetella horae is the latest Bordetella species to be associated with pertussis infection in humans, with higher rates in adolescents and adults. B. bronchiseptica can infect a variety of mammals, and it is rare to cause cough i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/689C12Q1/6844C12Q1/04
CPCC12Q1/689C12Q1/6844C12Q2600/166C12Q2531/119C12Q2522/101C12Q2521/507C12Q2521/319C12Q2563/107C12Q2545/113
Inventor 曹现涛陆亚平李彬魏光李拼
Owner 国药(武汉)医学实验室有限公司
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