Novel flower and fruit fragrance type red rice yeast natural fermented beverage and preparation method thereof
A natural fermentation and production method technology, applied in the field of food processing, can solve the problem of sacrificing the taste and aroma of fermented beverages, and achieve the effect of improving the use value, good sensory evaluation, and orange-red color
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Embodiment 1
[0021] Embodiment 1: the preferred of bacterial classification
[0022] Taking several strains of Monascus strains (Z505, GN, H4000, etc.) with strong aroma-producing ability in the laboratory as the research object, the slant seed activation, liquid seed cultivation and liquid fermentation culture of Monascus were carried out to obtain red yeast. Aspergillus fermentation broth, the content of volatile aroma substances was determined by gas chromatography-mass spectrometry.
[0023] Slope medium: glucose 6g, peptone 2g, soluble starch 3g, agar 3g, heated and dissolved in 100mL water, adjusted to pH 5.38 with lactic acid, distributed in test tubes, about 8mL / tube, sterilized at 115°C for 30min.
[0024] Secondary seed medium: Weigh 10g of fresh sweet potato pieces cut into small pieces, 2g of glucose, 0.6g of peptone, 0.3g of potassium dihydrogen phosphate, 0.2g of magnesium sulfate, add 100mL of water, adjust the pH to 5.39 with lactic acid, 121°C, 20min sterilization.
[00...
Embodiment 2
[0034] Slope medium: glucose 6g, peptone 2g, soluble starch 3g, agar 3g, heated and dissolved in 100mL water, adjusted to pH 5.32 with lactic acid, distributed in test tubes, about 8mL / tube, sterilized at 115°C for 30min.
[0035] Secondary seed medium: small pieces of fresh sweet potato 5g, glucose 1g, peptone 1g, potassium dihydrogen phosphate 0.5g, magnesium sulfate 0.05g, add water 100mL, adjust pH to 5.42 with lactic acid, sterilize at 121°C for 20min.
[0036] Fermentation medium: Weigh 10g of fresh sweet potato pieces cut into small pieces, 0.5g of potassium dihydrogen phosphate, and 0.05g of magnesium sulfate, add them to 100mL of water, adjust the pH to 5.83 with lactic acid, and sterilize at 121°C for 20min.
[0037] (1) Monascus purple Z505 was inoculated into the slant medium, and cultured in a 30°C incubator for 7 days to obtain first-grade seeds.
[0038] (2) The primary seeds in the slant medium were scraped and inoculated into the secondary seed medium, and cul...
Embodiment 3
[0042] Slope medium: glucose 6g, peptone 2g, soluble starch 3g, agar 3g, heated and dissolved in 100mL water, adjusted to pH 5.32 with lactic acid, distributed in test tubes, about 8mL / tube, sterilized at 115°C for 30min.
[0043] Secondary seed medium: small pieces of fresh sweet potato 5g, glucose 1g, peptone 1g, potassium dihydrogen phosphate 0.5g, magnesium sulfate 0.05g, add water 100mL, adjust pH to 5.39 with lactic acid, sterilize at 121°C for 20min.
[0044] Fermentation medium: Add 10g of small pieces of fresh sweet potato, 0.5g of potassium dihydrogen phosphate, and 0.05g of magnesium sulfate into 100mL of water, adjust the pH to 5.45 with lactic acid, and sterilize at 121°C for 20min.
[0045] (1) Monascus purple Z505 was inoculated into the slant medium, and cultured in a 30°C incubator for 7 days to obtain first-grade seeds.
[0046] (2) The primary seeds in the slant medium were scraped and inoculated into the secondary seed medium, and cultured at 30° C. and 200...
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