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Method for extracting mangiferin from mango leaves

A technology for mango leaves and mangiferin, which is applied in the field of extracting active components of technical plants, can solve the problems of reducing the extraction rate of mangiferin, and achieve the effects of shortening extraction time, improving extraction rate, and increasing cell pores.

Active Publication Date: 2021-09-21
永州德普瑞生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When using this method to extract mangiferin in mango leaves, the residual mangiferin in the extracted mango leaf filter residue is not fully extracted, which reduces the extraction rate of mangiferin

Method used

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  • Method for extracting mangiferin from mango leaves
  • Method for extracting mangiferin from mango leaves
  • Method for extracting mangiferin from mango leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A method for extracting mangiferin from mango leaves, comprising the following steps:

[0036] 1) Mango leaf puffing: select fresh mango leaves, remove dead and rotten leaves, weigh 2000g of screened mango leaves and place them in a low-temperature high-pressure puffing machine for puffing treatment. The temperature of puffing treatment is 75°C, and the puffing time is 1.5min. The puffing pressure is 25mPa, and after puffing, it is cooled to room temperature to obtain puffed mango leaves;

[0037] Before mango leaves are extracted with ethanol, low-temperature and high-pressure puffing treatment is used to loosen the tissue structure of mango leaves, increase the cell pores of mango leaves, help ethanol solution penetrate into cells, and effectively promote the production of mangiferin in cells. leaching;

[0038] 2) Ethanol extraction: Soak the puffed mango leaves in an ethanol solution for 1.5 hours according to the ratio of solid to liquid: 1:4, and extract for 1.5 ...

Embodiment 2

[0050] A method for extracting mangiferin from mango leaves, comprising the following steps:

[0051] 1) Mango leaf puffing: select fresh mango leaves, remove dead and rotten leaves, weigh 2000g of screened mango leaves and place them in a low-temperature and high-pressure puffing machine for puffing treatment. The pressure is 20mPa, and after puffing, it is cooled to room temperature to obtain puffed mango leaves;

[0052] 2) Ethanol extraction: Soak the puffed mango leaves in an ethanol solution for 1 hour according to the ratio of solid to liquid: 1:3, and extract for 1 hour to obtain an extract. Prepared by adding ascorbic acid to the solution;

[0053] 3) Primary filtration: filter the extract, and obtain primary filtrate and filter residue after filtration;

[0054] 4) Extraction: adding the primary filtrate into ether, and extracting with ether to obtain a primary crude extract;

[0055] 5) Drying and crushing; the filter residue is dried and crushed in a dryer, the ...

Embodiment 3

[0064] A method for extracting mangiferin from mango leaves, comprising the following steps:

[0065] 1) Mango leaf puffing: select fresh mango leaves, remove dead and rotten leaves, weigh 2000g of screened mango leaves, and place them in a low-temperature, high-pressure puffing machine for puffing treatment. The puffing temperature is 80°C, and the puffing time is 1.5min. The puffing pressure is 30mPa, and after puffing, it is cooled to room temperature to obtain puffed mango leaves;

[0066] 2) Ethanol extraction: Soak the puffed mango leaves in ethanol solution for 2 hours according to the ratio of material to liquid: 1:4 to obtain the extract solution. The ethanol solution is 95% ethanol according to the mass volume ratio Prepared by adding ascorbic acid to the solution;

[0067] 3) Primary filtration: filter the extract, and obtain primary filtrate and filter residue after filtration;

[0068] 4) Extraction: adding the primary filtrate into ether, and extracting with et...

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Abstract

The invention discloses a method for extracting mangiferin from mango leaves. The method comprises the following steps: 1) puffing the mango leaves to obtain puffed mango leaves; 2) performing extraction with ethanol to obtain a leaching solution; 3) performing primary filtration to obtain primary filtrate and filter residues; 4) performing extraction to obtain a primary crude extract; 5) performing drying and crushing; 6) activating strains: transferring aspergillus niger and active dry yeast into a PDA culture medium, and culturing in an incubator; 7) preparing a mixed bacterial suspension to obtain a mixed bacterial suspension; 8) material liquid mixing: mixing the crushed filter residues with the mixed bacterial suspension, and culturing the mixture in a shaking incubator to obtain a secondary crude extract; 9) performing secondary filtration to obtain a mangiferin extracting solution; and 10) subjecting the mangiferin extracting solution to concentration and freeze drying to obtain mangiferin. According to the method disclosed by the invention, the mango leaves are pretreated by adopting a low-temperature and high-pressure puffing technology, and the residual mangiferin in the filter residues is extracted by adopting the aspergillus niger and the active dry yeast, so that cell walls of the filter residues are broken, the dissolution of the mangiferin is promoted, and the extraction rate of the mangiferin is improved.

Description

technical field [0001] The invention relates to the technical field of extracting effective components of technical plants, in particular to a method for extracting mangiferin from mango leaves. Background technique [0002] Mango leaves The leaves of the Anacardiaceae plant mango are slightly sour, sweet, cool, and flat in nature, and have the functions of promoting qi, dispersing stagnation, and dispelling phlegm accumulation. symptom. Mango leaves contain mangiferin, protocatechuic acid, chlorophyll and other chemical components, which are the main source of mangiferin extraction. [0003] According to research, mangiferin has a good curative effect on chronic bronchitis; mangiferin has obvious anti-hepatotoxic and choleretic effects, and is often used in the treatment of hepatitis. Mangiferin has obvious toxic effects on liver cancer cell lines and can induce apoptosis of liver cancer cells . As a raw material, mangiferin has been widely used in medical and health pro...

Claims

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Application Information

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IPC IPC(8): C07D407/04
CPCC07D407/04
Inventor 周乾能王艳平
Owner 永州德普瑞生物科技股份有限公司
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